公开(公告)号：US20180024117A1

公开(公告)日：2018-01-25

申请号：US15648293

申请日：2017-07-12

申请人： Emulate Inc.

发明人： Daniel Levner ,  Kyung Jin Jang ,  Jacob Frase ,  Jordan Kerns ,  Antonio Varone ,  Dongeun Huh

IPC分类号： G01N33/50 ,  C12N5/071 ,  C12N5/00

CPC分类号： G01N33/5032 ,  C12M23/16 ,  C12M23/20 ,  C12M25/02 ,  C12N5/0018 ,  C12N5/0068 ,  C12N5/0658 ,  C12N5/067 ,  C12N2500/32 ,  C12N2521/00 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/10 ,  G01N33/5014 ,  G01N33/5044

摘要： Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed.