公开(公告)号：US20230340565A1

公开(公告)日：2023-10-26

申请号：US18097989

申请日：2023-01-17

申请人： 10X GENOMICS, INC.

发明人： Zahra Kamila BELHOCINE ,  Josephine Lee ,  Francesca MESCHI ,  Luz MONTESCLAROS ,  Katherine PFEIFFER ,  Andrew D. PRICE ,  Jerald SAPIDA ,  David SUKOVICH ,  Tobias Daniel WHEELER ,  Yifeng YIN

IPC分类号： C12Q1/6806

CPC分类号： C12Q1/6806

摘要： The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

公开(公告)号：US20200263232A1

公开(公告)日：2020-08-20

申请号：US16530930

申请日：2019-08-02

申请人： 10X GENOMICS, INC.

发明人： Jason Bell ,  Josephine Lee ,  Corey Nemec ,  Francesca Meschi

IPC分类号： C12Q1/6806 ,  C12N9/12 ,  C12N15/10

摘要： Methods and compositions to minimize barcode exchange during the preparation of barcoded next-generation sequencing libraries prepared from a single cell. The methods utilize oligonucleotides containing a 3′-terminated blocking group or sequences that prevent amplification or extension.

公开(公告)号：US20210115415A1

公开(公告)日：2021-04-22

申请号：US17114016

申请日：2020-12-07

申请人： 10X Genomics, Inc.

发明人： Josephine Lee ,  Samuel Marrs ,  Geoffrey McDermott ,  Francesca Meschi ,  Luz Montesclaros ,  Katherine Pfeiffer ,  Joseph Francis Shuga ,  Jessica Michele Terry ,  Solongo B. Ziraldo

IPC分类号： C12N9/12 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6844

摘要： Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition, greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes.

公开(公告)号：US11584954B2

公开(公告)日：2023-02-21

申请号：US16855177

申请日：2020-04-22

申请人： 10X GENOMICS, INC.

发明人： Zahra Kamila Belhocine ,  Josephine Lee ,  Francesca Meschi ,  Luz Montesclaros ,  Katherine Pfeiffer ,  Andrew D. Price ,  Jerald Sapida ,  David Sukovich ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC分类号： C12Q1/6806

摘要： The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

公开(公告)号：US20180312822A1

公开(公告)日：2018-11-01

申请号：US15975516

申请日：2018-05-09

申请人： 10X Genomics, Inc.

发明人： Josephine Lee ,  Samuel Marrs ,  Geoffrey McDermott ,  Francesca Meschi ,  Luz Montesclaros ,  Katherine Pfeiffer ,  Joseph Francis Shuga ,  Jessica Michele Terry ,  Solongo B. Ziraldo

IPC分类号： C12N9/12 ,  C12N15/10 ,  C12Q1/6806

摘要： Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition, greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes.