公开(公告)号：US20240110237A1

公开(公告)日：2024-04-04

申请号：US18227386

申请日：2023-07-28

Applicant: BASE PAIR BIOTECHNOLOGIES, INC.

Inventor： Jasmine Kaur ,  Rafal Drabek ,  George W. Jackson ,  Robert Batchelor ,  Alexander Chiu

IPC: C12Q1/6874 ,  C12N15/10 ,  C12N15/115 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6848 ,  C12Q1/6876 ,  C40B30/04

CPC classification number: C12Q1/6874 ,  C12N15/1048 ,  C12N15/115 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6848 ,  C12Q1/6876 ,  C40B30/04 ,  C12N2310/16 ,  C12N2330/31

Abstract: The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules.

公开(公告)号：US11807956B2

公开(公告)日：2023-11-07

申请号：US17068551

申请日：2020-10-12

Applicant: Twist Bioscience Corporation

Inventor： Anthony Cox ,  Sebastian Treusch ,  Siyuan Chen

IPC: C40B50/06 ,  C12N15/10 ,  G01N33/50 ,  C40B50/14 ,  C40B50/08 ,  C12N15/113 ,  C12Q1/6876 ,  C12Q1/6886

CPC classification number: C40B50/06 ,  C12N15/1079 ,  C12N15/1093 ,  C12N15/113 ,  C12Q1/6876 ,  C40B50/08 ,  C40B50/14 ,  G01N33/502 ,  G01N33/5029 ,  G01N33/5041 ,  C12N2320/30 ,  C12N2330/31 ,  C12Q1/6886 ,  C12Q2600/158

Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.

公开(公告)号：US20230250437A1

公开(公告)日：2023-08-10

申请号：US17727263

申请日：2022-04-22

Applicant: Caris Science, Inc.

Inventor： Valeriy Domenyuk ,  Tassilo Hornung ,  Heather O’Neill ,  Mark Miglarese ,  David Spetzler

IPC: C12N15/115 ,  C12N15/10 ,  C12Q1/6883 ,  C12Q1/6886

CPC classification number: C12N15/115 ,  C12N15/10 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12N2310/16 ,  C12N2330/31 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/158

Abstract: Methods and compositions are provided to identify oligonucleotides that bind targets of interest. The targets include tissues, cells, circulating biomarkers such as microvesicles, including those derived from various diseases. The oligonucleotides can be used in diagnostic and therapeutic applications.

公开(公告)号：US20230235320A1

公开(公告)日：2023-07-27

申请号：US18011271

申请日：2021-06-23

Applicant: CLARET BIOSCIENCE, LLC

Inventor： Christopher J. TROLL ,  Varsha RAO

IPC: C12N15/10 ,  C12Q1/6813 ,  C12Q1/6806

CPC classification number: C12N15/1093 ,  C12Q1/6813 ,  C12Q1/6806 ,  C12Q2600/16 ,  C12N2330/31

Abstract: The technology relates in part to methods and compositions for analyzing nucleic acid. In some aspects, the technology relates to methods and compositions for preparing a nucleic acid library from single-stranded nucleic acid fragments.

公开(公告)号：US11702677B2

公开(公告)日：2023-07-18

申请号：US16550092

申请日：2019-08-23

Applicant: The Regents of the University of Colorado

Inventor： Andrew Garst ,  Ryan T. Gill

IPC: C12N15/90 ,  C12N15/10 ,  C40B40/08 ,  C12N15/11 ,  C12N9/22

CPC classification number: C12N15/902 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/1082 ,  C12N15/1093 ,  C12N15/11 ,  C12N15/111 ,  C40B40/08 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2320/11 ,  C12N2320/12 ,  C12N2330/31

Abstract: Described herein are methods and vectors for rational, multiplexed manipulation of chromosomes within open reading frames (e.g., in protein libraries) or any segment of a chromosome in a cell or population of cells, in which various CRISPR systems are used.

公开(公告)号：US10059945B2

公开(公告)日：2018-08-28

申请号：US15443632

申请日：2017-02-27

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： Konrad Hochedlinger ,  Sihem Cheloufi ,  Marti Anne Borkent

IPC: C12N15/11 ,  C12N15/113 ,  C12N5/074

CPC classification number: C12N15/113 ,  C12N5/0696 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/531 ,  C12N2320/12 ,  C12N2330/31 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/998 ,  C12N2506/1307 ,  C12N2506/30

Abstract: Provided herein are methods for performing cellular reprogramming that include treatment of somatic cells with an inhibitor of CAF-1, Sumo2, Nutd21, or combinations thereof prior to or during a reprogramming procedure. Such inhibitors can improve both the speed and efficiency of cellular reprogramming. Inhibitors of the CAF-1 complex can also be used in the treatment of cancer.

公开(公告)号：US20180237782A1

公开(公告)日：2018-08-23

申请号：US15893532

申请日：2018-02-09

Applicant: Base Pair Biotechnologies, Inc.

Inventor： George W. Jackson

IPC: C12N15/115 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1048 ,  C12N15/115 ,  C12N2310/16 ,  C12N2330/31 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6848 ,  C12Q1/6876 ,  C40B30/04

Abstract: The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules, such as amino acids, such as citrulline, and to target molecules such as crystallin or its subunits, such as alpha crystallin B-chain (CRYAB).

公开(公告)号：US10053688B2

公开(公告)日：2018-08-21

申请号：US15682100

申请日：2017-08-21

Applicant: Twist Bioscience Corporation

Inventor： Anthony Cox ,  Siyuan Chen

IPC: C12N15/10 ,  C12Q1/68 ,  C40B40/06 ,  C12Q1/6806 ,  C12Q1/6816

CPC classification number: C12N15/1068 ,  C12N15/1093 ,  C12N15/111 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2330/31 ,  C12Q1/6806 ,  C12Q1/6816 ,  C40B40/06

Abstract: Disclosed herein are methods for the generation of nucleic acid libraries encoding for gRNA sequences. The gRNAs encoded by methods described herein may be single or double gRNA sequences. Methods described provide for the generation of gRNA libraries, as a DNA precursor or as a RNA transcription product, with improved accuracy and uniformity.

公开(公告)号：US10012652B2

公开(公告)日：2018-07-03

申请号：US15143435

申请日：2016-04-29

Applicant: Isis Innovation Limited

Inventor： Nicholas B. La Thangue ,  Susan Fotheringham

IPC: C12Q1/68 ,  G01N33/574 ,  C12Q1/6886 ,  C12N15/113

CPC classification number: G01N33/57496 ,  C12N15/111 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2320/10 ,  C12N2330/31 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Y305/01098 ,  G01N33/5011 ,  G01N33/574 ,  G01N33/57484 ,  G01N2333/4706 ,  G01N2333/91045 ,  G01N2333/98 ,  G01N2500/10 ,  G01N2800/52

Abstract: This invention relates to a novel screening method that identifies simple molecular markers that are predictive of whether a particular disease condition is responsive to a specific treatment. Also, a method of diagnosing the susceptibility of an individual suffering from a disease to treatment with an HDAC inhibitor is provided. Also provided is a method of treating a proliferative disease or a condition which involves a change in cell differentiation or growth rate in a patient.

公开(公告)号：US09982278B2

公开(公告)日：2018-05-29

申请号：US15630909

申请日：2017-06-22

Applicant: THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE

Inventor： Ryan T. Gill ,  Andrew Garst

IPC: C12N15/09 ,  C12N15/90 ,  C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12N15/63 ,  C12N15/64 ,  C12N15/70 ,  C12N15/74 ,  C12N15/79 ,  C12N15/81 ,  C12N15/82 ,  C12N15/85

CPC classification number: C12N15/902 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/1082 ,  C12N15/1093 ,  C12N15/11 ,  C12N15/111 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2320/11 ,  C12N2320/12 ,  C12N2330/31 ,  C40B40/08

Abstract: Described herein are methods and vectors for rational, multiplexed manipulation of chromosomes within open reading frames (e.g., in protein libraries) or any segment of a chromosome in a cell or population of cells, in which various CRISPR systems are used.