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公开(公告)号：US11559778B2
公开(公告)日：2023-01-24
申请号：US16039256
申请日：2018-07-18
IPC分类号： B01J19/00 , C12N15/10 , C40B50/18 , C40B50/00 , C40B50/14 , C12N15/66 , C12N15/74 , C40B40/06 , C12N15/09
摘要： De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
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公开(公告)号：US11185837B2
公开(公告)日：2021-11-30
申请号：US16535777
申请日：2019-08-08
IPC分类号： B01J19/00 , C12N15/10 , C40B50/18 , C40B50/00 , C40B40/06 , C40B50/14 , C12N15/66 , C12N15/74 , C12N15/09
摘要： De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
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公开(公告)号：US10894959B2
公开(公告)日：2021-01-19
申请号：US15921537
申请日：2018-03-14
发明人： Anthony Cox , Siyuan Chen
IPC分类号： C12N15/10 , C12Q1/6806
摘要： Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The nucleic acid sequence may encode for all or part of a reference domain of a CAR. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may be designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of a disease, such as cancer.
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公开(公告)号：US09981239B2
公开(公告)日：2018-05-29
申请号：US15135434
申请日：2016-04-21
发明人： William Banyai , Bill James Peck , Andres Fernandez , Siyuan Chen , Pierre Indermuhle , Eugene P. Marsh
CPC分类号： B01J19/0046 , B01J2219/00317 , B01J2219/00497 , B01J2219/00511 , B01J2219/00522 , B01J2219/00585 , B01J2219/00596 , B01J2219/00635 , B01J2219/00637 , B01J2219/00662 , B01J2219/00722 , B01L3/50857 , C12N15/1093 , C12Q2563/159 , C12Q2565/513 , C12Q2565/629
摘要： Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.
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公开(公告)号：US10272410B2
公开(公告)日：2019-04-30
申请号：US14885965
申请日：2015-10-16
IPC分类号： B01J19/00 , C12N15/10 , C40B50/18 , C12N15/66 , C12N15/74 , C40B50/00 , C40B40/06 , C40B50/14 , C12N15/09
摘要： Provided herein are devices for the manufacturing of high-quality oligonucleic acids. Longer nucleic acids, e.g., genes, can be synthesized in parallel using microfluidic assemblies described herein. Devices described herein include silicon plates having a plurality of channels in fluid communication with a plurality of microchannels. The number of microchannels and dimensions of the microchannels provide for rapid exchange of chemical exposure during de novo synthesis of oligonucleic acids.
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公开(公告)号：US20180264428A1
公开(公告)日：2018-09-20
申请号：US15991992
申请日：2018-05-29
CPC分类号： B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00378 , B01J2219/00497 , B01J2219/00585 , B01J2219/00587 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00619 , B01J2219/00623 , B01J2219/00637 , B01J2219/00709 , B01J2219/00722 , C12N15/09 , C12N15/1093 , C12N15/1096 , C12N15/66 , C12N15/74 , C40B40/06 , C40B50/00 , C40B50/14 , C40B50/18
摘要： De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
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公开(公告)号：US09833761B2
公开(公告)日：2017-12-05
申请号：US14885962
申请日：2015-10-16
CPC分类号： B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00378 , B01J2219/00497 , B01J2219/00585 , B01J2219/00587 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00619 , B01J2219/00623 , B01J2219/00637 , B01J2219/00709 , B01J2219/00722 , C12N15/09 , C12N15/1093 , C12N15/1096 , C12N15/66 , C12N15/74 , C40B40/06 , C40B50/00 , C40B50/14 , C40B50/18
摘要： De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
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公开(公告)号：US09403141B2
公开(公告)日：2016-08-02
申请号：US14885963
申请日：2015-10-16
CPC分类号： B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00378 , B01J2219/00497 , B01J2219/00585 , B01J2219/00587 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00619 , B01J2219/00623 , B01J2219/00637 , B01J2219/00709 , B01J2219/00722 , C12N15/09 , C12N15/1093 , C12N15/1096 , C12N15/66 , C12N15/74 , C40B40/06 , C40B50/00 , C40B50/14 , C40B50/18
摘要： De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
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公开(公告)号：US11807956B2
公开(公告)日：2023-11-07
申请号：US17068551
申请日：2020-10-12
发明人： Anthony Cox , Sebastian Treusch , Siyuan Chen
IPC分类号： C40B50/06 , C12N15/10 , G01N33/50 , C40B50/14 , C40B50/08 , C12N15/113 , C12Q1/6876 , C12Q1/6886
CPC分类号： C40B50/06 , C12N15/1079 , C12N15/1093 , C12N15/113 , C12Q1/6876 , C40B50/08 , C40B50/14 , G01N33/502 , G01N33/5029 , G01N33/5041 , C12N2320/30 , C12N2330/31 , C12Q1/6886 , C12Q2600/158
摘要： Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.
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公开(公告)号：US11732294B2
公开(公告)日：2023-08-22
申请号：US16590301
申请日：2019-10-01
IPC分类号： C12Q1/68 , C12Q1/6869 , G16B40/10 , C40B30/10 , C40B70/00
CPC分类号： C12Q1/6869 , C40B30/10 , C40B70/00 , G16B40/10
摘要： Provided herein are compositions, methods and systems relating to libraries of polynucleotides such that the libraries allow for accurate and efficient hybridization after binding to target sequences. Further provided herein are probes, blockers, additives, buffers, and methods that result in improved hybridization. Such compositions and methods are useful for improvement of Next Generation Sequencing applications, such as reducing off-target binding or reducing workflow times.
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