公开(公告)号：US12226745B2

公开(公告)日：2025-02-18

申请号：US16403339

申请日：2019-05-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Abraham Rosenbaum ,  Collyn Seeger ,  Jeremy Gray ,  Hua Yu

IPC: C12Q1/6853 ,  B01J19/00 ,  C12Q1/6844 ,  C40B50/14

Abstract: The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.

公开(公告)号：US20240378459A1

公开(公告)日：2024-11-14

申请号：US18781511

申请日：2024-07-23

Applicant: Twist Bioscience Corporation

Inventor： Brian Wayne BRAMLETT ,  Bill James PECK

IPC: G06N3/123 ,  B01J19/00 ,  C12N15/09 ,  C12N15/10 ,  C12Q1/6869 ,  C40B50/14 ,  G06F16/00 ,  G06F17/40 ,  G06N99/00 ,  G11C11/00 ,  G11C11/56 ,  G11C13/00 ,  G11C13/02 ,  G16B30/00 ,  G16B50/20

Abstract: Provided herein are compositions, devices, systems and methods for generation and use of biomolecule-based information for storage. Further provided are devices-having addressable electrodes controlling polynucleotide synthesis (deprotection, extension, or cleavage, etc.) The compositions, devices, systems and methods described herein provide improved storage, density, and retrieval of biomolecule-based information.

公开(公告)号：US11692223B2

公开(公告)日：2023-07-04

申请号：US16846028

申请日：2020-04-10

Applicant: Illumina Cambridge Limited

Inventor： Kevin L. Gunderson ,  Jingwei Bai ,  Matthew William Kellinger ,  John M. Beierle ,  Jonathan Mark Boutell ,  Roberto Rigatti ,  Maria Candelaria Rogert Bacigalupo ,  Boyan Boyanov ,  Klaus Maisinger

IPC: C12P19/34 ,  C12Q1/6874 ,  B01J19/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6853 ,  C40B40/06 ,  C40B50/14

CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6853 ,  C40B40/06 ,  C40B50/14 ,  B01J2219/00317 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00637 ,  B01J2219/00722 ,  C12Q1/6874 ,  C12Q2565/513 ,  C12Q2565/543

Abstract: The present disclosure relates to the field of molecular biology and more specifically to microarrays and methods, including methods for modifying immobilized capture primers comprising: a) contacting a substrate comprising a plurality of immobilized capture primers with a plurality of template nucleic acids under conditions sufficient for hybridization to produce one or more immobilized template nucleic acids, and b) extending one or more immobilized capture primers to produce one or more immobilized extension products complementary to the one or more template nucleic acid.

公开(公告)号：US11559778B2

公开(公告)日：2023-01-24

申请号：US16039256

申请日：2018-07-18

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William Banyai ,  Bill James Peck ,  Andres Fernandez ,  Siyuan Chen ,  Pierre Indermuhle

IPC: B01J19/00 ,  C12N15/10 ,  C40B50/18 ,  C40B50/00 ,  C40B50/14 ,  C12N15/66 ,  C12N15/74 ,  C40B40/06 ,  C12N15/09

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

公开(公告)号：US11453875B2

公开(公告)日：2022-09-27

申请号：US15575803

申请日：2016-05-27

Applicant: ILLUMINA CAMBRIDGE LIMITED

Inventor： Niall Anthony Gormley ,  Avgousta Ioannou ,  Rosamond Jackson ,  Natalie Morrell

IPC: C12N15/10 ,  C12Q1/6806 ,  C12N9/22 ,  C40B50/14

Abstract: Presented herein are methods and compositions surface-based tagmentation. In particular embodiments, methods of preparing an immobilized library of fragmented and tagged DNA molecules on a solid surface are presented. In particular embodiments, the solid surface comprises immobilized transposomes in a dried format, suitable for reconstitution upon contact with liquid, such as a liquid sample.

公开(公告)号：US20210394178A1

公开(公告)日：2021-12-23

申请号：US17236283

申请日：2021-04-21

Applicant: Plexium, Inc.

Inventor： Kandaswamy Vijayan ,  Andrew Boyd MacConnell ,  Joseph Franklin Rokicki

IPC: B01L3/00 ,  C12N15/10 ,  C12Q1/6869 ,  C40B50/04 ,  C40B50/14 ,  C12Q1/6876 ,  C40B30/06 ,  C40B50/16 ,  C40B30/04

Abstract: This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

公开(公告)号：US11185837B2

公开(公告)日：2021-11-30

申请号：US16535777

申请日：2019-08-08

Applicant: Twist Bioscience Corporation

Inventor： William Banyai ,  Bill James Peck ,  Andres Fernandez ,  Siyuan Chen ,  Pierre Indermuhle

IPC: B01J19/00 ,  C12N15/10 ,  C40B50/18 ,  C40B50/00 ,  C40B40/06 ,  C40B50/14 ,  C12N15/66 ,  C12N15/74 ,  C12N15/09

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

公开(公告)号：US11162131B2

公开(公告)日：2021-11-02

申请号：US16252558

申请日：2019-01-18

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Rui Chen ,  Toumy Guettouche ,  Loida Navarro ,  Aaron Richardson

IPC: C12P19/34 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12N15/10 ,  C12Q1/683 ,  C12Q1/6869 ,  C40B40/06 ,  C40B50/14

Abstract: The invention is a novel method of making and using a template for nucleic acid sequencing. The templates include circular and linear templates with symmetric and asymmetric adaptors. The methods include utilizing the templates in an asymmetric fashion.

公开(公告)号：US11104942B2

公开(公告)日：2021-08-31

申请号：US15772404

申请日：2016-10-28

Applicant: GUANGDONG MAIJINJIA BIOTECHNOLOGIES CO., LTD.

Inventor： Thorleif Moeller

IPC: C12P19/34 ,  C12Q1/686 ,  A61J1/20 ,  C12Q1/6832 ,  C12N15/10 ,  A61J1/10 ,  A61J1/14 ,  C12Q1/6811 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6874 ,  C40B20/02 ,  C40B40/08 ,  C40B50/14

Abstract: A method for identification of the most abundant oligonucleotide species in a library of oligonucleotides comprising more than 106 oligonucleotide species, wherein the oligonucleotides of the library of oligonucleotides are not inherently capable of exponential amplification by PCR and include a coding sequence, which is characteristic of one oligonucleotide specie of the library of oligonucleotides and only one fixed sequence, which is present in a plurality of oligonucleotide species of the library of oligonucleotides, said fixed sequence being located on a 5′ side of the coding sequence, and wherein the method comprises specifically amplifying the sequence of a hybridised oligonucleotide species using the steps of: incubating the library of oligonucleotides under conditions of hybridization such as to allow complementary coding sequences to hybridize and form hybridized oligonucleotide species; extending a 3′ end of one or more of the hybridised oligonucleotide species, having only one fixed sequence, to provide extended sequences having an extended region, such that the extended region generates a second fixed sequence thereby forming extended sequences having two fixed sequences; amplifying extended sequences comprising two fixed sequences using PCR to provide amplified sequences, and optionally identifying an identity of the amplified sequences thereby revealing an identity of the hybridised oligonucleotide species in the step of incubating. The library of oligonucleotides is selected from the group of a library of encoded molecules, a library of aptamers, a library of reporter sequences derived from a library of encoded molecules, and a library of aptamers.

公开(公告)号：US11084037B2

公开(公告)日：2021-08-10

申请号：US16140494

申请日：2018-09-24

Applicant: Plexium, Inc.

Inventor： Kandaswamy Vijayan ,  Andrew Boyd MacConnell ,  Joseph Franklin Rokicki

IPC: C40B50/16 ,  B01L3/00 ,  C12N15/10 ,  C12Q1/6869 ,  C40B50/04 ,  C40B50/14 ,  C12Q1/6876 ,  C40B30/06 ,  C40B30/04

Abstract: This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.