公开(公告)号：US20240003057A1

公开(公告)日：2024-01-04

申请号：US18368496

申请日：2023-09-14

Applicant: Illumina, Inc.

Inventor： Molly He ,  Michael Previte ,  Misha Golynskiy ,  Matthew William Kellinger ,  Sergio Peisajovich ,  Jonathan Mark Boutell

IPC: C40B50/16 ,  B01L3/00 ,  B01J19/00 ,  C40B20/04 ,  C12N15/10

CPC classification number: C40B50/16 ,  B01L3/5085 ,  B01J19/0046 ,  B01L3/54 ,  C40B20/04 ,  C12N15/1062 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/1068 ,  B01L2300/021 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00626 ,  B01J2219/00743

Abstract: A method of characterizing candidate agents including steps of (a) providing a library of candidate agents attached to nucleic acid tags; (b) contacting the library with a solid support to attach the candidate agents to the solid support, whereby an array of candidate agents is formed; (c) contacting the array with a screening agent, wherein one or more candidate agents in the array react with the screening agent; (d) detecting the array to determine that at least one candidate agent in the array reacts with the screening agent; (e) sequencing the nucleic acid tag to determine the tag sequences attached to candidate agents in the array; and (f) identifying the at least one candidate agent in the array that reacts with the screening agent based on the tag sequence that is attached to the at least one candidate agent.

公开(公告)号：US20220297125A1

公开(公告)日：2022-09-22

申请号：US17592390

申请日：2022-02-03

Applicant: 1859, Inc.

Inventor： Devon CAYER ,  Andrew MACCONNELL ,  Pavel CHUBUKOV ,  Ramesh RAMJI ,  Sean STROMBERG

IPC: B01L3/00 ,  C12Q1/686 ,  C12N15/10 ,  C12Q1/6844 ,  C12Q1/6876 ,  C40B50/06 ,  C40B50/16

Abstract: Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.

公开(公告)号：US20210277444A1

公开(公告)日：2021-09-09

申请号：US17095578

申请日：2020-11-11

Applicant: 10X GENOMICS, INC.

Inventor： Zachary Bent ,  Elliott Meer ,  Daniel Riordan ,  Paul Ryvkin ,  Niranjan Srinivas ,  Jessica Terry ,  Alex Gagnon

IPC: C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/6834 ,  C40B20/04 ,  C40B50/16 ,  C40B50/18 ,  G06K19/06

Abstract: The present disclosure provides methods of generating supports (e.g., beads) comprising barcode molecules coupled thereto. A barcode molecule coupled to a support may comprise a barcode sequence and a functional sequence. A barcode molecule may be generated using two or more ligation reactions in a combinatorial fashion. A support comprising two or more different barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.

公开(公告)号：US11014948B2

公开(公告)日：2021-05-25

申请号：US15925509

申请日：2018-03-19

Applicant: The Royal Institution for the Advancement of Learning/McGill University ,  Hong Kong Polytechnic University

Inventor： Masad J. Damha ,  Matthew Hassler ,  Tak-Hang Chan ,  Mallikarjuna Reddy Nandyala ,  Robert Alexander Donga

IPC: C07D233/60 ,  C07F9/54 ,  C07F9/655 ,  C07F9/6558 ,  C07H19/02 ,  C07H21/00 ,  C07H21/02 ,  C40B50/16 ,  C40B80/00

Abstract: The invention relates to the chemical synthesis of oligonucleotides, e.g., oligoribonucleotides. In another aspect, the invention relates to compounds of formula (II): processes for making these compounds, and the use thereof in the chemical synthesis of oligonucleotides, e.g., oligoribonucleotides. The invention also relates to methods of synthesis of oligomers, including but not limited to oligopeptides, oligosaccharides and oligonucleotides, particularly oligoribonucleotides and also oligodeoxyribonucleotides, in solution systems, and ionic tag linkers for use in methods provided herein.

公开(公告)号：US10946383B2

公开(公告)日：2021-03-16

申请号：US16534922

申请日：2019-08-07

Applicant: Plexium, Inc.

Inventor： Kandaswamy Vijayan ,  Andrew Boyd MacConnell ,  Joseph Franklin Rokicki

IPC: B01L3/00 ,  C12N15/10 ,  C12Q1/6869 ,  C40B50/04 ,  C40B50/14 ,  C12Q1/6876 ,  C40B30/06 ,  C40B50/16 ,  C40B30/04

Abstract: This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

公开(公告)号：US10934584B2

公开(公告)日：2021-03-02

申请号：US15959984

申请日：2018-04-23

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Eric Hans Vermaas ,  Mahdieh Khosroheidari ,  Claire Bevis-Mott

IPC: C12Q1/6874 ,  C12Q1/6834 ,  C12Q1/6855 ,  C12Q1/6806 ,  C40B50/18 ,  C40B20/04 ,  C40B50/16 ,  C12Q1/6809 ,  C12Q1/6816 ,  C40B70/00 ,  C40B80/00

Abstract: The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by modifying or blocking 5′ and 3′ ends of pooled indexed polynucleotides from multiple samples, with an optional exonuclease treatment, prior to amplification and sequencing.

公开(公告)号：US10828643B2

公开(公告)日：2020-11-10

申请号：US16534886

申请日：2019-08-07

Applicant: Plexium, Inc.

Inventor： Kandaswamy Vijayan ,  Andrew Boyd MacConnell ,  Joseph Franklin Rokicki

IPC: C12N15/10 ,  C12Q1/6869 ,  C12Q1/6876 ,  B01L3/00 ,  C40B50/04 ,  C40B50/14 ,  C40B30/06 ,  C40B50/16 ,  C40B30/04

Abstract: This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

公开(公告)号：US10436778B2

公开(公告)日：2019-10-08

申请号：US14208481

申请日：2014-03-13

Applicant: PLEXBIO CO., LTD.

Inventor： Dean Tsao ,  Chin-Shiou Huang

IPC: G01N33/543 ,  C40B30/04 ,  C40B40/04 ,  C40B50/16

Abstract: Disclosed herein are compositions comprising beads with unique identifiers for storing information about a multiplex assay as well as methods for using the same in multiplex chemical and biological assays.

公开(公告)号：US20190249226A1

公开(公告)日：2019-08-15

申请号：US16230936

申请日：2018-12-21

Applicant: 10X GENOMICS, INC.

Inventor： Zachary Bent ,  Elliott Meer ,  Daniel Riordan ,  Paul Ryvkin ,  Niranjan Srinivas ,  Jessica Terry ,  Alex Gagnon

IPC: C12Q1/6806 ,  G06K19/06 ,  C12Q1/6811 ,  C12Q1/6834 ,  C40B20/04 ,  C40B50/16 ,  C40B50/18

Abstract: The present disclosure provides methods of generating supports (e.g., beads) comprising barcode molecules coupled thereto. A barcode molecule coupled to a support may comprise a barcode sequence and a functional sequence. A barcode molecule may be generated using two or more ligation reactions in a combinatorial fashion. A support comprising two or more different barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.

公开(公告)号：US20190127777A1

公开(公告)日：2019-05-02

申请号：US15822186

申请日：2017-11-26

Applicant: BioInventors & Entrepreneurs Network, LLC

Inventor： Michael Seul ,  Ghazala Hashmi

IPC: C12Q1/6806 ,  C40B20/04 ,  G01N33/58 ,  C12N15/10 ,  C40B50/10 ,  C40B50/16

Abstract: Disclosed is a novel method of allele profiling, or nucleic acid sieving, with pooled Sanger sequencing as a first (aka “screening”) stage; where the first step is: amplifying a single sequence, delineated by forward and reverse primers which may represent a single exon, or a segment thereof, or a contiguous stretch of multiple exons and introns. The amplicons produced from a pool of samples include the amplified sequence, and these are next converted into fragments in the standard Sanger labeling reaction. Ambiguities will appear as superposed peaks at any heterozygous position of interest, as the origin of the variant signal cannot be uniquely attributable to a specific sample, or samples, in the pool. These ambiguities may be resolved by the allele profiling process; or, resolution can be done with source-tagged primers generating source-tagged amplicons, which generate position shifts in labels, which can be decoded to resolve the ambiguities.