公开(公告)号：US12146134B2

公开(公告)日：2024-11-19

申请号：US15996222

申请日：2018-06-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC: C12N15/10 ,  B01F33/302 ,  B01F33/3031 ,  B01J19/00 ,  B01L3/00 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/04 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6876 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N33/536 ,  G01N33/543 ,  G01N33/68 ,  B01F23/41 ,  B01L7/00 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/6869 ,  G01N21/64 ,  G01N33/542

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

公开(公告)号：US11834756B2

公开(公告)日：2023-12-05

申请号：US17019121

申请日：2020-09-11

Applicant: Google LLC

Inventor： Annalisa Marie Pawlosky ,  Michael Gibbons ,  Sara Ahadi ,  Shirley Jing Shao ,  Anna Le ,  Ali Bashir ,  Marc Berndl ,  Michelle Therese Hoerner Dimon ,  Lauren Schiff

IPC: C12Q1/68 ,  C40B30/04 ,  C40B70/00 ,  C12Q1/6806 ,  C40B40/04 ,  C12Q1/6869

CPC classification number: C40B30/04 ,  C12Q1/6806 ,  C12Q1/6869 ,  C40B40/04 ,  C40B70/00 ,  C12Q2525/205

Abstract: This disclosure describes methods and compositions for protein and peptide sequencing.

公开(公告)号：US20230323565A1

公开(公告)日：2023-10-12

申请号：US18025107

申请日：2021-09-08

Applicant: CRYSTALSFIRST GMBH

Inventor： Gerhard KLEBE ,  Stefan MERKL ,  Serghei GLINCA ,  Kan FU

IPC: C40B40/04 ,  C30B7/14

CPC classification number: C40B40/04 ,  C30B7/14

Abstract: Subject-matter of the present invention is an array comprising soaking solutions for soaking a biological macromolecular crystal. Further, the subject of the invention is a rule-based method of selecting specific soaking solution compositions having a specific composition comprising composite solute(s), water (w), crystallization solution (crs) and/or organic solvent(s). Additionally, the subjects matter of the invention the soaking solutions obtained by the method of the invention and a screening method for small molecules comprising molecular probes, fragments and drug-size molecules using the soaking solutions and the use of the soaking solutions in a screening method for small molecules on a macromolecular crystal.

公开(公告)号：US11738321B2

公开(公告)日：2023-08-29

申请号：US15611662

申请日：2017-06-01

Applicant: The Regents of the University of California ,  National Technology & Engineering Solutions of Sandia, LLC

Inventor： Todd Duncombe ,  Trent R. Northen ,  Kai Deng ,  Anup K. Singh

IPC: G01N33/58 ,  B01J19/00 ,  C07F5/00 ,  C40B70/00 ,  C40B20/04 ,  C40B40/04

CPC classification number: B01J19/0046 ,  C07F5/003 ,  B01J2219/00547 ,  B01J2219/00581 ,  B01J2219/00702 ,  C40B20/04 ,  C40B40/04 ,  C40B70/00 ,  G01N2458/40 ,  G01N2560/00

Abstract: Disclosed herein are methods for tracking solutions, (e.g., reaction conditions in solutions). In some embodiments, the method comprises: contacting a first lanthanide-chelator complex to a first solution to generate a first barcoded solution, wherein the first lanthanide-chelator complex comprises a first lanthanide chelated by a first chelator; contacting a second lanthanide-chelator complex to a second solution to generate a second barcoded solution, wherein the second lanthanide-chelator complex comprises a second lanthanide chelated by a second chelator; mixing the first barcoded solution and the second barcoded solution to form one or more mixtures; and identifying the first lanthanide ions in the mass spectrum and the second lanthanide ions in the mass spectrum to track the condition of each of the one or more mixtures.

公开(公告)号：US20230219043A1

公开(公告)日：2023-07-13

申请号：US18178965

申请日：2023-03-06

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Darren Roy LINK ,  John Brian HUTCHISON ,  Michael L. SAMUELS ,  Michael WEINER

IPC: B01F23/41 ,  C40B50/08 ,  G01N33/50 ,  C40B40/04 ,  B01J19/00 ,  B01F25/433 ,  G01N15/14 ,  B01L3/00 ,  B01F33/3011 ,  B01L7/00 ,  B01F101/23 ,  G01N15/10

CPC classification number: B01F23/41 ,  C40B50/08 ,  G01N33/5008 ,  C40B40/04 ,  B01J19/0046 ,  B01F25/433 ,  G01N15/1459 ,  B01L3/502761 ,  B01F33/3011 ,  B01F25/4336 ,  B01L2400/0487 ,  B01L2300/0654 ,  B01J2219/00286 ,  B01J2219/00479 ,  B01L7/52 ,  B01L2300/0636 ,  B01J2219/0059 ,  B01J2219/00702 ,  B01L2200/027 ,  B01J2219/00599 ,  B01F2101/23 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00592 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01L2300/0864 ,  B01L2400/0415 ,  G01N2500/00 ,  B01J2219/00743 ,  B01J2219/0074 ,  B01L2300/0645 ,  B01J2219/00657 ,  B01J2219/00418 ,  B01J2219/00351 ,  B01L2300/0867 ,  B01J2219/0065 ,  B01L2300/0681 ,  G01N2015/1006 ,  B01J2219/00664 ,  Y02A90/10

Abstract: The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

公开(公告)号：US11453621B2

公开(公告)日：2022-09-27

申请号：US16061136

申请日：2016-12-09

Applicant: ELECTROPHORETICS LIMITED

Inventor： Andrew Hugin Thompson

IPC: G01N33/58 ,  C07B59/00 ,  C07D403/12 ,  G01N33/68 ,  C12Q1/6872 ,  C40B40/04

Abstract: The present invention relates to a set of two or more mass labels, wherein each mass label comprises the formula: X-L-M-Re wherein X is a reporter moiety having an exact mass, L is a bond cleavable by collision in a mass spectrometer, M is a mass modifier, and Re is a) a reactive functionality for attaching the mass label to an analyte or b) the analyte, wherein each mass label in the set has an integer mass, wherein each mass label in the set has the same integer mass, and wherein the set comprises two or more subsets of mass labels, each subset comprising one, two or more mass labels, and wherein, when the subset comprises two or more mass labels, the exact mass of the reporter moiety X of each mass label in the subset is different from the exact mass of the reporter moiety X of the mass labels in the same subset and in all other subsets, and wherein each mass label is distinguishable by mass spectrometry.

公开(公告)号：US20200049695A1

公开(公告)日：2020-02-13

申请号：US16487478

申请日：2018-02-22

Applicant: UNIVERSITY OF SOUTHAMPTON

Inventor： Ali TAVASSOLI ,  Catrin SOHRABI ,  Martin FISCHLECHNER

IPC: G01N33/50 ,  C07K1/04 ,  C40B40/04 ,  C07K7/64 ,  C12N15/10

Abstract: A method for co-compartmentalising a cyclic polypeptide with a polynucleotide encoding the cyclic polypeptide, comprising the steps of a) forming a compartment containing a polynucleotide encoding the cyclic polypeptide, b) expressing a polypeptide from the polynucleotide, and c) cyclising the polypeptide. Co-compartmentalised cyclic polypeptides and encoding polynucleotides. Libraries of co-compartmentalised cyclic polypeptide and encoding polynucleotide. Methods for screening libraries of co-compartmentalised cyclic polypeptide and encoding polynucleotide. Incorporation of non-canonical nucleic acids into such libraries.

公开(公告)号：US10551382B2

公开(公告)日：2020-02-04

申请号：US16199822

申请日：2018-11-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Michael L. Samuels

IPC: G01N33/58 ,  G01N33/573 ,  B01F5/06 ,  B01J19/00 ,  B01F13/00 ,  B01L3/00 ,  B01F3/08 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  B01L7/00 ,  G01N21/64

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US20180355350A1

公开(公告)日：2018-12-13

申请号：US15996222

申请日：2018-06-01

Applicant: Raindance Technologies, Inc.

Inventor： Darren Roy Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC: C12N15/10 ,  G01N33/68 ,  B01F13/00 ,  G01N33/543 ,  G01N33/536 ,  C40B70/00 ,  C40B60/12 ,  C40B60/08 ,  C40B50/08 ,  C40B40/04 ,  C40B30/04 ,  C12Q1/6876 ,  C12Q1/6874 ,  C12Q1/686 ,  C07K1/04 ,  B82Y30/00 ,  B82Y5/00 ,  B01L3/00 ,  B01J19/00 ,  G01N33/542 ,  G01N21/64 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/6804 ,  B01L7/00 ,  B01F3/08

CPC classification number: C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

公开(公告)号：US09879300B2

公开(公告)日：2018-01-30

申请号：US14302238

申请日：2014-06-11

Applicant: UVic Industry Partnerships Inc.

Inventor： Fraser Hof ,  Samuel Minaker ,  Kevin Daze ,  Sara Tabet ,  Manuel Ma

IPC: C12Q1/25 ,  C07C309/43 ,  C07C309/44 ,  C07C309/50 ,  C07C309/60 ,  C12Q1/00 ,  C40B40/12 ,  C40B40/04 ,  G01N33/68

CPC classification number: C12Q1/25 ,  C07C309/43 ,  C07C309/44 ,  C07C309/50 ,  C07C309/60 ,  C07C2603/92 ,  C12Q1/00 ,  C40B40/04 ,  C40B40/12 ,  G01N33/6803 ,  G01N2440/00

Abstract: Disclosed embodiments concern an array for use in identifying or identifying and quantifying analytes in a sample using a macrocyclic sensor comprising a macrocyclic compound and a detectable moiety. The disclosed array may be used to discriminate among various analytes based on different features, such as post-translational modifications, isomeric post-translational modifications, and the peptide sequence around post-translational modifications. Also disclosed is a method for identifying analytes comprising a post-translational modification, as well as an enzymatic assay using the disclosed macrocyclic sensor.