公开(公告)号：US11958912B2

公开(公告)日：2024-04-16

申请号：US17062302

申请日：2020-10-02

申请人： Hoffmann-La Roche Inc.

发明人： Joerg Moelleken ,  Michael Molhoj ,  Christian Gassner ,  Manuel Endesfelder ,  Joerg-Thomas Regula

IPC分类号： G01N31/00 ,  C07K16/46 ,  G01N33/53 ,  G01N33/536

CPC分类号： C07K16/461 ,  C07K16/468 ,  G01N33/53 ,  G01N33/536 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/92

摘要： Herein is reported a method for determining the binding interaction with a multimeric antigen of an antibody of the human IgG1 subclass that has at least two binding sites specifically binding to the antigen comprising the steps of 1) determining the binding affinity of the antibody for the multimeric antigen, and 2) incubating a mixture comprising the antibody and a polypeptide that is derived from lysine-gingipain of Porphyromonas gingivalis under conditions and for a time sufficient to cleave the antibody into Fabs and Fc-region, and determining the binding affinity of the Fabs of the antibody for the multimeric, whereby the binding affinity of the antibody to the multimeric antigen to be affinity-driven if the binding affinity determined in both steps are comparable and to be avidity-driven if the binding affinity determined in both steps are different.

公开(公告)号：US11898194B2

公开(公告)日：2024-02-13

申请号：US16872855

申请日：2020-05-12

申请人： TOPPAN PRINTING CO., LTD.

发明人： Takumi Hirase ,  Yoichi Makino ,  Yosuke Horiuchi

IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  B01L3/00 ,  C12Q1/686 ,  G01N33/536

CPC分类号： C12Q1/6806 ,  B01L3/5027 ,  C12Q1/686 ,  G01N33/536 ,  B01L2200/0689 ,  B01L2300/0829

摘要： A method for detecting a target molecule, including supplying a liquid dispersion of a structural substance including a target molecule into a well array such that the structural substance is introduced into wells of the well array, supplying a sealing solution into the well array such that a layer of the sealing solution is formed on the liquid dispersion in the wells, and that the liquid dispersion is sealed in the wells, extracting the target molecule from the structural substance, and detecting the target molecule.

公开(公告)号：US11821897B2

公开(公告)日：2023-11-21

申请号：US16448571

申请日：2019-06-21

申请人： Hoffmann-La Roche Inc.

发明人： Regula Buser ,  Sandra Grau-Richards ,  Virginie Steinhart

IPC分类号： G01N33/542 ,  C12N15/85 ,  G01N21/64 ,  G01N33/543 ,  G01N33/569 ,  G01N33/58 ,  G01N33/53 ,  G01N33/536 ,  G01N33/533

CPC分类号： G01N33/542 ,  C12N15/85 ,  G01N21/6408 ,  G01N21/6428 ,  G01N33/53 ,  G01N33/533 ,  G01N33/536 ,  G01N33/54306 ,  G01N33/56966 ,  G01N33/56972 ,  G01N33/586 ,  G01N2021/6439

摘要： The present invention relates to a new cell based assay for determining antibody or ligand binding and function using lipid-like compounds capable of spontaneous integration into cell membranes.

公开(公告)号：US11668707B2

公开(公告)日：2023-06-06

申请号：US16468963

申请日：2017-12-12

申请人： Siemens Healthcare Diagnostics Inc.

发明人： Jacques Brunelle

IPC分类号： G01N33/536 ,  G01N21/27 ,  G01N21/31 ,  G01N21/77 ,  G01N33/72 ,  G01N35/10 ,  G01N21/82 ,  G01J3/10 ,  G01J3/00 ,  G01N33/70 ,  G01N33/68 ,  G01J3/42

CPC分类号： G01N33/536 ,  G01N21/272 ,  G01N21/3151 ,  G01N21/77 ,  G01N33/721 ,  G01N35/1002 ,  B01L2300/0663 ,  G01J3/00 ,  G01J3/10 ,  G01J3/42 ,  G01N21/82 ,  G01N33/6827 ,  G01N33/70 ,  G01N33/726 ,  G01N2021/3129 ,  G01N2021/3148 ,  G01N2021/825 ,  G01N2201/061

摘要： Analyzers and methods for making and using analyzers are described such as a method in which multiple absorption readings of a liquid assay are obtained by a photodetector using multiple light sources having at least three separate and independent wavelength ranges and with each of the absorption readings taken at a separate instant of time. Using at least one processor and calibration information of the liquid assay, an amount of at least two analytes within the liquid assay using the multiple absorption readings is determined.

公开(公告)号：US20190204200A1

公开(公告)日：2019-07-04

申请号：US16093566

申请日：2017-04-17

申请人： Takuro ENDO ,  METAWATER CO., LTD.

发明人： Takuro ENDO ,  Kyungju KIM ,  Dabide YAMAGUCHI

IPC分类号： G01N15/06 ,  G01N33/569 ,  G01N15/14

CPC分类号： G01N15/06 ,  C12Q1/06 ,  G01N15/14 ,  G01N15/1429 ,  G01N21/64 ,  G01N33/18 ,  G01N33/536 ,  G01N33/569 ,  G01N2015/0693 ,  Y02A50/451

摘要： Providing a method of estimating the number of microparticles such as microorganisms in a sample, performing complicated operations. The method comprises counting by constant flow the number of target microorganisms contained in the sample at a predetermined flow rate, sectioning measurement data obtained as a result of the constant flow counting into a predetermined number of sections by a predetermined unit time for a section, counting the number of sections in which microorganisms are detected and the number of sections in which they are not detected, in the predetermined number of sections; and estimating the number of microorganisms in the sample, by a statistical method from the flow rate of the sample in the constant flow counting step, the predetermined number of sections and the predetermined unit time in the sectioning step, and the number of sections in which microorganisms are detected in the counting step.

公开(公告)号：US20180143194A1

公开(公告)日：2018-05-24

申请号：US15563667

申请日：2016-04-04

申请人： THE UNIVERSITY OF ROCHESTER ,  EMORY UNIVERSITY

发明人： John Linforth DAISS ,  Edward M. SCHWARZ ,  Kohei NISHITANI ,  Sandeep SOIN ,  Frances Eun-Hyung LEE

IPC分类号： G01N33/569 ,  G01N33/50 ,  G01N33/536 ,  C07K16/12

CPC分类号： G01N33/56938 ,  C07K16/1271 ,  C07K2317/14 ,  G01N33/5091 ,  G01N33/536

摘要： Provided herein are compositions and methods for detecting and/or diagnosing a Staphylococcus aureus (S. aureus) infection in a subject. It is a surprising finding of the present invention that an S. aureus infection may be detected by using one or more antigens selected from the group consisting of an IsdA antigen, IsdB antigen, CHIPS antigen, and a SCIN antigen. In some embodiments, these S. aureus antigens are used to detect antibodies secreted by the plasmablasts from the subject.

公开(公告)号：US20180045716A1

公开(公告)日：2018-02-15

申请号：US15557078

申请日：2016-03-10

申请人： Siemens Healthcare Diagnostics Inc.

发明人： Spencer LIN ,  Christopher REAMER ,  Ryan SPEARS

IPC分类号： G01N33/531 ,  G01N33/536 ,  G01N33/543

CPC分类号： G01N33/531 ,  G01N33/52 ,  G01N33/536 ,  G01N33/54306 ,  G01N33/54353

摘要： Processes for quantifying an amount of functional groups immobilized on a solid support are described herein. The processes allow for determining whether sufficient functional groups are provided on a solid support for the attachment of a first binding pair member for the detection of a target analyte.

公开(公告)号：US09872916B2

公开(公告)日：2018-01-23

申请号：US14390945

申请日：2013-04-05

申请人： University of North Texas

发明人： Mohammad A. Omary ,  Sreekar Marpu

IPC分类号： A61K47/48 ,  A61K33/38 ,  G01N33/50 ,  G01N33/536 ,  A61K49/00 ,  B22F1/00 ,  A61K9/50 ,  B82Y30/00

CPC分类号： A61K47/59 ,  A61K9/501 ,  A61K9/5026 ,  A61K33/38 ,  A61K47/58 ,  A61K47/61 ,  A61K49/0065 ,  A61K49/0091 ,  B22F1/0018 ,  B22F1/0062 ,  B82Y30/00 ,  G01N33/5005 ,  G01N33/536 ,  Y10T436/25

摘要： The present invention includes photochemical method of making hybrid metal-polymer microparticles in an aqueous, biocompatible solution by providing a metal (I) composition and one or more polymeric materials; applying an electromagnetic radiation to the metal (I) composition; converting the metal (I) composition to a metal (0) composition; forming one or more hybrid metal-polymer microparticles from the metal (0); capping the one or more hybrid metal-polymer microparticles; and stabilizing the one or more hybrid metal-polymer microparticles with the one or more polymeric materials to prevent agglomeration.

公开(公告)号：US20180008944A1

公开(公告)日：2018-01-11

申请号：US15544464

申请日：2015-10-07

申请人： ULVAC, INC.

发明人： Tomomitsu OZEKI

IPC分类号： B01F13/10 ,  G01N33/536 ,  B01F7/16 ,  B01F7/00 ,  B01F15/00 ,  G01N35/02 ,  G01N1/38

CPC分类号： B01F13/1022 ,  B01F7/00841 ,  B01F7/1665 ,  B01F7/18 ,  B01F15/00006 ,  B01F15/00389 ,  B01F15/00454 ,  B01F15/00461 ,  B01F2215/0037 ,  C12M23/12 ,  C12M27/02 ,  G01N1/38 ,  G01N33/536 ,  G01N35/00 ,  G01N35/026 ,  G01N2035/00544

摘要： [Object] To provide a mixing device capable of accurately mixing a solution in a multi-well plate.[Solving Means] A mixing device 1 is configured to be attachable to a multi-well plate 30 and includes a casing 100, a plurality of stirrers 11, a plurality of motors 12 as a drive portion, and a mounting portion 16. The casing 100 includes a main surface portion 101 facing an upper surface 301 of the multi-well plate 30. The stirrers 11 protrude from the main surface portion 101 toward wells 31 of the multi-well plate 30. The motors 12 are disposed to the casing 100 and rotate the stirrers 11 about axes thereof. The mounting portion 16 is provided to the casing 100 and is mounted to the multi-well plate 30 to position the casing 100 on the multi-well plate 30.

公开(公告)号：US20170362630A1

公开(公告)日：2017-12-21

申请号：US15696089

申请日：2017-09-05

申请人： KYOTO UNIVERSITY

发明人： Tai KIUCHI ,  Naoki WATANABE ,  Takushi MIYOSHI ,  Ryo SASAKI

IPC分类号： C12Q1/02 ,  G01N33/48 ,  G01N21/64 ,  G01N33/536 ,  C07K1/13

CPC分类号： C12Q1/02 ,  C07K1/13 ,  G01N21/64 ,  G01N33/48 ,  G01N33/53 ,  G01N33/536

摘要： An observation method of a sample containing a target substance, the observation method including an imaging step in which a step of obtaining a speckle image including, as a speckle, light emitted from a luminescent substance in which a medium is brought into contact with the sample is performed a plurality of times so as to obtain a plurality of speckle images, the medium containing a probe that contains the luminescent substance emitting light and that repeatedly binds to and dissociates from the target substance directly and specifically, and an observation image generation step of generating an observation image of the target substance in the sample from the plurality of speckle images, wherein a half-life of a probe-target complex formed by binding between the probe and the target substance is equal to or more than 10 milliseconds and equal to or less than 3 seconds.