公开(公告)号：US11906507B2

公开(公告)日：2024-02-20

申请号：US17210957

申请日：2021-03-24

申请人： The Procter & Gamble Company

发明人： Karl Shiqing Wei ,  Feng Yue ,  Hechuan Yu

IPC分类号： G01N33/483 ,  G01N33/569

CPC分类号： G01N33/4833 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56961 ,  G01N33/56983

摘要： A method of taking a skin sample can include placing an adhesive onto a portion of skin and lifting the adhesive from the skin. A skin sample may then be tested while still on the adhesive, for example, by inoculating the sample with a bacterium, fungus, virus, or a combination.

公开(公告)号：US20230392217A1

公开(公告)日：2023-12-07

申请号：US18034064

申请日：2021-10-15

申请人： INSTITUT PASTEUR KOREA

发明人： Soojin JANG ,  Yunmi LEE

IPC分类号： C12Q1/689 ,  G01N33/569 ,  C07K16/12

CPC分类号： C12Q1/689 ,  G01N33/56938 ,  C07K16/1271 ,  C12Q2600/106 ,  C12Q2600/158 ,  G01N2800/52

摘要： The present invention relates to a composition for diagnosing the susceptibility of Staphylococcus aureus to antibiotics, and a use thereof. Particularly, it has been ascertained that sosA is a marker specific to Staphylococcus aureus, and that the expression level of sosA is exhibited similarly to antibiotic susceptibility/resistance properties so as to be usable as a means for diagnosing the susceptibility/resistance of Staphylococcus aureus to antibiotics, and the present invention diagnoses the susceptibility/resistance of Staphylococcus aureus present in samples to antibiotics by using antibodies specifically binding to protein SosA, and thus provides, as a means for diagnosing the susceptibility of Staphylococcus aureus to antibiotics, a composition containing an agent for measuring the mRNA expression level of the sosA gene or an agent for measuring the expression level of the SosA protein.

公开(公告)号：US20230295248A1

公开(公告)日：2023-09-21

申请号：US18153727

申请日：2023-01-12

申请人： NEW YORK UNIVERSITY

发明人： Victor J. TORRES ,  Ashley L. DUMONT

IPC分类号： C07K14/31 ,  C07K16/12 ,  G01N33/50 ,  G01N33/569 ,  C12Q1/02 ,  G01N33/68

CPC分类号： C07K14/31 ,  C07K16/1271 ,  G01N33/5035 ,  G01N33/56938 ,  C12Q1/025 ,  G01N33/68 ,  A61K39/00

摘要： Disclosed herein are isolated and purified Staphylococcus aureus bi-component leukocidin, referred to herein as LukAB, and its components LukA and LukB, antibodies specific to LukA, antibodies specific to LukB, therapeutic compositions containing LukA and/or LukB, or anti-LukA and/or anti-LukB antibodies, uses of the compositions to treat acute inflammatory conditions or S. aureus infection, methods for identifying inhibitors of LukAB-mediated cytotoxicity of human phagocytes, and methods for using LukAB as a marker to predict severity of S. aureus infection.

公开(公告)号：US11718651B2

公开(公告)日：2023-08-08

申请号：US17318343

申请日：2021-05-12

申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Alexander L. Watters ,  Donald E. Ingber ,  Mark J. Cartwright ,  Michael Super ,  Martin Rottman ,  Evangelia Murray ,  Brendon Dusel

IPC分类号： C07K14/47 ,  G01N33/68 ,  G01N33/569 ,  A61K47/42 ,  A61K38/00

CPC分类号： C07K14/4737 ,  A61K47/42 ,  C07K14/4726 ,  G01N33/569 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56944 ,  G01N33/6854 ,  A61K38/00 ,  C07K2319/30 ,  C07K2319/33 ,  G01N2333/4737

摘要： Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can bind or capture of a microbe or microbial matter thereof, and can thus be used in various applications, such as diagnosis or treatment of an infection caused by microbes in a subject or any environmental surface.

公开(公告)号：US20190219512A1

公开(公告)日：2019-07-18

申请号：US16246172

申请日：2019-01-11

申请人： Clifford H. Kern, III ,  Edward M. Yokley ,  William Tison Wyatt ,  Morton Greene

发明人： Clifford H. Kern, III ,  Edward M. Yokley ,  William Tison Wyatt ,  Morton Greene

IPC分类号： G01N21/77 ,  G01N33/02 ,  A61K39/085

CPC分类号： G01N21/77 ,  A61K39/085 ,  C07K14/31 ,  C07K16/1271 ,  C12Q1/14 ,  G01N33/02 ,  G01N33/56938 ,  G01N2021/7756

摘要： A bio-sensor device for the electro-chemical detection of a bacterial pathogen, the device including a sample chamber and an electronic data module. The sample chamber includes electrical probes to detect pathogenic antigens in a sample containing the bacterial pathogen. The electrical probes detect a reaction voltage corresponding to an antigen-antibody reaction occurring when the pathogenic antigens come into contact with an antibody specific for pathogenic antigens present in a reaction medium in the sample chamber and contacted by the electrical probes. The electronic data module detects and processes electrical signals from the conductive electrical probes corresponding to an amount of the antigen present in the sample, wherein the reaction voltage is detected at the time of the reaction.

公开(公告)号：US20180321238A1

公开(公告)日：2018-11-08

申请号：US15770249

申请日：2016-10-24

申请人： Thomas BRUDERER ,  Madhavi MURANJAN

发明人： Thomas BRUDERER ,  Madhavi MURANJAN

IPC分类号： G01N33/569 ,  C07K16/12 ,  B01L3/00 ,  G01N33/52

CPC分类号： G01N33/56938 ,  B01L3/5023 ,  C07K16/00 ,  C07K16/1271 ,  G01N33/52

摘要： A subtractive immunoassay for detecting the presence or absence of first and second analytes in a sample includes in the following order the steps of: a) conjugating a first detector antibody to the first analyte to tag the first analyte with a first detector tag; b) conjugating a first capture antibody to the first analyte to capture the first analyte to completely deplete the first analyte from the sample; c) conjugating a second detector antibody to the second analyte in the depleted sample to tag the second analyte with a second detector tag; d) conjugating a second capture antibody to the second analyte to capture the second analyte from the sample; e) detecting presence or absence of the first and second detector tags at the site of the first and second capture antibody, respectively, and thereby detecting the presence or absence of the first and second analytes in the sample.

公开(公告)号：US20180289791A1

公开(公告)日：2018-10-11

申请号：US16013494

申请日：2018-06-20

申请人： SOCPRA - SCIENCES ET GÉNIE, s.e.c.

发明人： François Malouin ,  Marianne Allard ,  Christian Lebeau Jacob ,  Brian Geoffrey Talbot ,  Daniel Scholl ,  Pierre Lacasse ,  Moussa S. Diarra ,  Céline Ster

IPC分类号： A61K39/085 ,  G01N33/569 ,  C12Q1/689 ,  A61K39/00

CPC分类号： A61K39/085 ,  A61K39/00 ,  A61K2039/522 ,  A61K2039/552 ,  A61K2039/55566 ,  C12Q1/689 ,  C12Q2600/158 ,  G01N33/56938

摘要： Agents, compositions, methods and kits useful for the treatment and diagnosis of Staphylococcal intramammary infection are disclosed. The agents, compositions, methods and kits are derived from genes expressed during Staphylococcal intramammary infection, and more particularly genes SACOL0029, SACOL0264, SACOL0442, SACOL0718, SACOL0720, SACOL1353, SACOL1416, SACOL1611, SACOL1944, SACOL2144, SACOL2365 or SACOL2599, based on the gene nomenclature from the Staphylococcus aureus COL (SACOL) genome.

公开(公告)号：US20180143200A1

公开(公告)日：2018-05-24

申请号：US15570410

申请日：2016-05-03

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  UNIVERSITE D'AIX-MARSEILLE ,  UNIVERSITE PARIS SUD

发明人： Sam DUKAN ,  Boris VAUZEILLES ,  Jordi MAS PONS ,  Aurélie BARON

IPC分类号： G01N33/58 ,  C12Q1/04 ,  G01N33/569

CPC分类号： G01N33/582 ,  C12Q1/02 ,  C12Q1/04 ,  G01N33/56905 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56961 ,  Y02A50/52

摘要： The invention relates to a method for labeling specifically living microorganisms in a sample comprising microorganisms, the method comprising the steps of: a) incubating said microorganisms of said sample with at least one modified monosaccharide compound comprising a first reactive chemical group capable to chemically react with a second reactive group, so that a residue bearing said first reactive group is incorporated into such microorganisms, and b) contacting said residue incorporated in the microorganisms, with a labeling molecule comprising a said second reactive group, for generating the chemical reaction of said first reactive group of said residue incorporated within said living microorganisms with said second reactive group of said labeling molecule, resulting in a covalent link, characterized in that the said modified monosaccharide compound has the following formula (I′), or a salt thereof: —X can be O, NH or S, preferably O and NH, and —R1 and R2 can be independently H, OH, NH2, OH and NH2 being substituted or not by protecting groups thereof, preferably substituted by alkyl, hydroxyalkyl, acyl, formyl or imidoyl groups, and —R3 is H or an alkyl chain in C1 to C4, each carbon being substituted or not substituted by OH or NH2 substituted or not by protecting groups thereof, preferably by alkyl, hydroxyalkyl, acyl, formyl or imidoyl groups, and—at least one of X, R1, R2 and R3 groups, preferably R3, being substituted by a said first reactive group Ra.

公开(公告)号：US20180143195A1

公开(公告)日：2018-05-24

申请号：US15569143

申请日：2016-04-28

申请人： DENKA SEIKEN CO., LTD

发明人： Yuji SAITO ,  Daisuke KATO

IPC分类号： G01N33/569 ,  C07K14/195 ,  C07K1/36

CPC分类号： G01N33/56938 ,  C07K1/36 ,  C07K14/195 ,  C12N1/00 ,  C12N1/06 ,  C12Q1/04 ,  G01N33/543 ,  G01N33/569

摘要： This invention provides a method for easily collecting antigens possessed by microorganisms without the use of special equipment. The method for collecting microbial antigens comprises: allowing a specimen containing microorganisms to pass through a filter membrane with a pore diameter that does not allow microorganisms to pass therethrough; capturing the microorganisms in the specimen on the filter membrane; applying a microbial destruction reagent capable of microbial membrane destruction to the filter membrane comprising the microorganisms captured thereon to destruct the captured microorganisms on the filter membrane; and collecting antigens in the filtrate.

公开(公告)号：US09944694B2

公开(公告)日：2018-04-17

申请号：US15103586

申请日：2014-12-12

申请人： RIJKSUNIVERSITEIT GRONINGEN ,  ACADEMISCH ZIEKENHUIS GRONINGEN

发明人： Jan Maarten Van Dijl ,  Girbe Buist ,  Hendrik Petrus Jozef Bonarius ,  Neeltje Margaretha Kooi ,  Goffe Sytse Elsinga ,  Herman Groen

IPC分类号： C07K16/12 ,  G01N33/569 ,  A61K39/00

CPC分类号： C07K16/1271 ,  A61K2039/505 ,  A61K2039/507 ,  C07K2317/21 ,  C07K2317/33 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/565 ,  C07K2317/70 ,  C07K2317/94 ,  G01N33/56938 ,  G01N2469/10

摘要： The invention relates to the field of immunology and vaccine development. In particular, it relates to antibodies and fragments thereof against Staphylococcus aureus and to therapeutic and diagnostic uses thereof. Provided is an isolated antibody or a functional fragment thereof, which binds to an epitope of the Immunodominant Staphylococcal antigen A (IsaA), wherein said antibody comprises at least four Isa A epitope-binding CDR sequences selected from the group consisting of SEQ ID NO: 1; SEQ ID NO: 2; SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 5; SEQ ID NO: 6, and functional equivalents thereof having an amino acid sequence that is at least 85% identical to an amino acid sequence of SEQ ID NO: 1-6.