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公开(公告)号：US11788125B2
公开(公告)日：2023-10-17
申请号：US17324944
申请日：2021-05-19
Inventor： Alexander Green
IPC: C07H21/02 , C12Q1/6841 , C12N15/115 , G01N33/53
CPC classification number: C12Q1/6841 , C12N15/115 , G01N33/5308 , C12N2310/16 , C12N2310/531 , C12Q2525/205 , C12Q2525/301
Abstract: Provided herein are imaging probes and systems and methods employing such imaging probes for real-time, label-free, multiplexed imaging of RNAs in living cells. More particularly, aptamer-based sensors (“aptasensors”) and molecular fuses comprising multiple aptasensors are genetically encoded imaging probes comprising RNA-target binding sequence and an intramolecular reconfiguration sequence. The probe is configured such that binding of a RNA target by the RNA-target binding sequence triggers the intramolecular reconfiguration sequence to reconfigure such that an optically detectable output is generated by the probe.
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公开(公告)号：US11773430B2
公开(公告)日：2023-10-03
申请号：US16342722
申请日：2017-10-17
Inventor： Alexander Green , Duo Ma , Anli Tang
IPC: C12Q1/6825 , C12Q1/6816
CPC classification number: C12Q1/6825 , C12Q1/6816 , Y02A50/30 , C12Q1/6825 , C12Q2525/205 , C12Q1/6816 , C12Q2525/205 , C12Q2565/625
Abstract: Methods and compositions for detecting the presence of a pathogen are described. In particular, this document provides a method of detecting a pathogen-associated nucleic acid in a biological sample of a subject, where the method comprises using one or more unimolecular aptamer-based sensors comprising an aptamer-fluorophore complex and an amplification step to detect the pathogen-associated nucleic acid. Methods specific for detecting the presence of malaria and other mosquito-borne virus infections are also provided.
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公开(公告)号：US20180245068A1
公开(公告)日：2018-08-30
申请号：US15753343
申请日：2016-08-18
Applicant: GE HEALTHCARE UK LIMITED
Inventor： Jeffrey Kenneth HORTON , Peter James TATNELL
IPC: C12N15/10 , C12N15/115 , C12Q1/6834
CPC classification number: C12N15/1048 , C12N15/115 , C12N2310/16 , C12N2320/13 , C12N2330/31 , C12Q1/6834 , C12Q2525/204 , C12Q2531/113 , C12Q2525/205 , C12Q2525/207
Abstract: The present invention relates to a method and kit for analyte detection. More precisely the invention relates to a method of detecting an analyte, comprising storing short single stranded nucleic acids (10-100 nt), such as aptamers and micro RNA, on a solid support at ambient temperature; and subsequent amplification of said nucleic acids for detection of analyte(s).
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公开(公告)号：US20180224444A1
公开(公告)日：2018-08-09
申请号：US15827441
申请日：2017-11-30
Applicant: SomaLogic, Inc.
Inventor： Glenn Sanders , Stephan Kraemer , Evaldas Katilius
IPC: G01N33/543 , G01N33/68 , C07H21/00 , G01N33/53 , C12Q1/6811
CPC classification number: G01N33/54393 , C07H21/00 , C12Q1/6804 , C12Q1/6811 , C12Q1/6837 , G01N33/5308 , G01N33/543 , G01N33/54306 , G01N33/68 , G01N33/6803 , G01N2570/00 , C12Q2525/113 , C12Q2525/205 , C12Q2561/101
Abstract: The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer) where the aptamer-aptamer interactions are significantly reduced or eliminated while maintaining the aptamer-target interaction.
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公开(公告)号：US20180155725A1
公开(公告)日：2018-06-07
申请号：US15878161
申请日：2018-01-23
Applicant: SomaLogic, Inc.
Inventor： Dominic Zichi , Sheri K. Wilcox , Chris Bock , Daniel J. Schneider , Bruce Eaton , Larry Gold
IPC: C12N15/115 , C12N15/10 , G01N33/53
CPC classification number: C12N15/115 , C12N9/6429 , C12N15/1048 , C12N2310/16 , C12N2310/314 , C12N2310/315 , C12N2310/321 , C12N2310/322 , C12N2310/333 , C12N2310/334 , C12N2310/335 , C12N2310/336 , C12N2330/31 , C12Q1/6811 , C12Q1/6816 , C12Q1/6832 , C12Q1/6834 , G01N33/5308 , G01N33/58 , C12Q2525/205 , C12Q2565/514 , C12Q2525/161 , C12Q2537/101 , C12Q2523/313
Abstract: The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently crosslinking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.
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公开(公告)号：US09873879B2
公开(公告)日：2018-01-23
申请号：US15359879
申请日：2016-11-23
Applicant: TAGCYX BIOTECHNOLOGIES
Inventor： Ichiro Hirao , Michiko Hirao , Rie Yamashige , Shigeyuki Yokoyama
IPC: C12N15/115 , A61K48/00 , C12N15/10 , C12Q1/68 , C12N15/11 , C12N15/113 , G01N33/53
CPC classification number: C12N15/115 , A61K48/00 , C12N15/1048 , C12N15/111 , C12N15/113 , C12N2310/12 , C12N2310/16 , C12N2310/33 , C12N2310/331 , C12N2320/13 , C12Q1/6869 , G01N33/5308 , C12Q2525/101 , C12Q2525/205
Abstract: An object of the present invention is to develop and provide a method for efficiently producing a nucleic acid aptamer, particularly, a DNA aptamer, having higher specificity and binding activity against a target substance than those of nucleic acid aptamers obtained by conventional methods. The present invention provides a transcribable or replicable nucleic acid aptamer comprising a natural nucleotide and a non-natural nucleotide having an artificial base-pairable artificial base. The present invention also provides a method for sequencing a non-natural nucleotide-containing single-stranded nucleic acid molecule selected from a single-stranded nucleic acid library.
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公开(公告)号：US20180002734A1
公开(公告)日：2018-01-04
申请号：US15634874
申请日：2017-06-27
Applicant: BioTex, Inc.
Inventor： George Jackson , Christopher Quan
CPC classification number: C12Q1/6804 , C12Q1/6844 , C12Q2525/205 , C12Q2537/161 , G01N33/5308 , C12Q2537/163 , C12Q2525/207
Abstract: This invention relates to methods for molecular detection, particularly to methods utilizing target-specific molecular probes. In exemplary embodiments, target-specific molecular probes include single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) aptamers. In general, the molecular probe may bind with relatively high specificity to a given target. In one aspect, a method for molecular detection comprises a molecular probe paired to a reporter molecule wherein the molecular probe impairs the amplification of the reporter molecule in the absence of the target molecule.
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公开(公告)号：US20170335314A1
公开(公告)日：2017-11-23
申请号：US15601449
申请日：2017-05-22
Applicant: TriBiotica LLC
Inventor： Ian Dunn , Matthew Lawler
IPC: C12N15/10 , A61K31/713 , C12N15/115
CPC classification number: C12N15/1048 , A61K31/713 , C12N15/115 , C12N2310/16 , C12N2310/531 , C12Q1/6811 , C12Q1/6816 , C12Q2525/205
Abstract: The present disclosure provides nucleic acid aptamers, nucleic acid aptamers hybridized to haplomers, methods of using nucleic acid aptamers to present template sequences, where the aptamers bind to target molecules unique to specific cellular targets, for the purpose of nucleic acid-templated assembly of molecules with desired functions.
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公开(公告)号：US20170321251A1
公开(公告)日：2017-11-09
申请号：US15597917
申请日：2017-05-17
Applicant: APPRISE BIO, INC.
Inventor： Garry P. Nolan
IPC: C12Q1/68
CPC classification number: C12Q1/6806 , C12Q1/6816 , C12Q1/686 , C12Q2521/501 , C12Q2525/161 , C12Q2525/185 , C12Q2525/197 , C12Q2525/205 , C12Q2537/143 , C12Q2563/149 , C12Q2565/514
Abstract: The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.
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10.发明授权
公开(公告)号：US09803233B2
公开(公告)日：2017-10-31
申请号：US14649998
申请日：2013-12-05
Inventor： Itamar Willner , Zhang Zhanxia , Dora Balogh
IPC: C12Q1/68 , A61K31/4745 , A61K9/51 , B82Y15/00
CPC classification number: C12Q1/6823 , A61K9/5115 , A61K31/4745 , B82Y15/00 , C12Q2521/345 , C12Q2563/155 , C12Q2563/159 , C12Q2525/205 , C12Q2521/319 , C12Q2525/301 , C12Q2521/301
Abstract: The present invention relates to a porous substrate comprising at least one active agent entrapped within said pores of said substrate; wherein said pores are capped by at least one nucleic acid sequence; said agent is being released by a triggered reaction of said capping sequence with at least one analyte (biomarker) thereby allowing said capping to be cleaved from said pore. The invention further relates to methods of manufacturing said substrate, uses thereof for the controlled administration of active agents and diagnostic of conditions in a patient.
