公开(公告)号：US20190176152A1

公开(公告)日：2019-06-13

申请号：US16325922

申请日：2017-08-23

申请人： 10X Genomics, Inc.

发明人： Rajiv Bharadwaj ,  Tobias Daniel Wheeler ,  Kevin Ness ,  Benjamin Hindson

IPC分类号： B01L3/00 ,  B01F13/00 ,  B01F3/08

CPC分类号： B01L3/502784 ,  B01F3/0807 ,  B01F3/0811 ,  B01F13/0086 ,  B01F2215/0037 ,  B01J2/06 ,  B01L2200/0673 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/16 ,  C12Q1/6844 ,  C12Q2531/113 ,  C12Q2563/179 ,  C12Q2565/629

摘要： A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

公开(公告)号：US20190099721A1

公开(公告)日：2019-04-04

申请号：US15958258

申请日：2018-04-20

申请人： Tamkang University

发明人： Tung-Wen Cheng ,  Su-En Wu ,  Yi-Chun Lin ,  Chung-Kai Chang

IPC分类号： B01D67/00 ,  B01D71/02 ,  B01D69/02

CPC分类号： B01D67/0041 ,  B01D69/02 ,  B01D71/02 ,  B01D2323/08 ,  B01D2325/04 ,  B01D2325/36 ,  B01F3/0807 ,  B01F3/0853 ,  B01F5/0473 ,  B01F5/0476 ,  B01F7/00458

摘要： A method for manufacturing a porous membrane includes: mixing silicon carbide powders and a coagulant to form a first mixture; adding a sintering aid to the first mixture to form a second mixture; compressing the second mixture; and sintering the compressed second mixture. More particularly, the coagulant is in an amount of 1% to 3% by weight of the silicon carbide powders and the sintering aid is in an amount of 10% by weight of the first mixture.

公开(公告)号：US20190094226A1

公开(公告)日：2019-03-28

申请号：US16199822

申请日：2018-11-26

申请人： Raindance Technologies, Inc.

发明人： Darren Roy Link ,  Michael L. Samuels

IPC分类号： G01N33/573 ,  B01L3/00 ,  C12Q1/6804 ,  C12Q1/25 ,  C12Q1/6818 ,  G01N33/58 ,  G01N33/542 ,  C40B40/04 ,  C40B50/08 ,  B01F13/00 ,  B01F5/06 ,  B01J19/00 ,  G01N33/50 ,  B01F3/08 ,  C40B60/10 ,  C12Q1/6827 ,  B01L7/00 ,  G01N21/64

CPC分类号： G01N33/573 ,  B01F3/0807 ,  B01F5/0646 ,  B01F5/0653 ,  B01F13/0062 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00351 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00599 ,  B01J2219/0065 ,  B01J2219/00657 ,  B01J2219/00664 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/0074 ,  B01J2219/00743 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N21/6428 ,  G01N21/6445 ,  G01N33/5008 ,  G01N33/542 ,  G01N33/582 ,  G01N2500/00

摘要： The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US20180353913A1

公开(公告)日：2018-12-13

申请号：US16105042

申请日：2018-08-20

申请人： Raindance Technologies, Inc.

发明人： Darren Roy Link ,  Brian Hutchison ,  Michael L. Samuels ,  Michael Weiner

IPC分类号： B01F3/08 ,  G01N33/50 ,  C40B50/08 ,  C40B40/04

CPC分类号： B01F3/0807 ,  B01F5/0646 ,  B01F5/0653 ,  B01F13/0062 ,  B01F2215/0037 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00351 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00599 ,  B01J2219/0065 ,  B01J2219/00657 ,  B01J2219/00664 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/0074 ,  B01J2219/00743 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  C40B40/04 ,  C40B50/08 ,  G01N15/1459 ,  G01N33/5008 ,  G01N2015/1006 ,  G01N2500/00 ,  Y02A90/26

摘要： The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

公开(公告)号：US20180328315A1

公开(公告)日：2018-11-15

申请号：US15776483

申请日：2016-11-21

申请人： Roman TANIEL

发明人： Roman TANIEL

IPC分类号： F02M25/022 ,  B01F3/08 ,  B01F7/00 ,  B01F7/04 ,  B01F13/10 ,  C10L1/32

CPC分类号： F02M25/0228 ,  B01F3/0807 ,  B01F7/008 ,  B01F7/00908 ,  B01F7/00916 ,  B01F7/04 ,  B01F13/0836 ,  B01F13/1027 ,  B01F2003/0842 ,  B01F2003/0849 ,  B01F2215/0088 ,  C10L1/328 ,  F02D41/0025 ,  F02M43/04

摘要： What is proposed is an emulsifying system with an emulsifying device and an injection nozzle as well as an emulsifying device for producing a water-fuel emulsion for an internal combustion engine, wherein the emulsifying device is embodied as a rotor-stator emulsifying device and/or fluid flow machine and/or is connected or connectable directly to an injection nozzle. The emulsifying device has a housing and a shaft, the shaft being drivable in a contactless manner, the housing having a guide apparatus having a plurality of guide channels for guiding the flow, and/or the housing being made at least partially from fiber composite material. Moreover, an emulsifying method for producing a water-fuel emulsion is proposed in which water and fuel are fed to a rotor-stator emulsifying device and/or fluid flow machine for producing the water-fuel emulsion, and/or in which water and fuel are premixed in a first emulsifying stage and fed via a guide apparatus having a plurality of guide channels to a second emulsifying stage.

公开(公告)号：US20180272296A1

公开(公告)日：2018-09-27

申请号：US15996253

申请日：2018-06-01

申请人： Raindance Technologies, Inc.

发明人： Darren Roy Link ,  Michael Weiner ,  David Marran ,  Jonathan M. Rothberg

IPC分类号： B01F3/08 ,  G01N35/08 ,  G01N27/327 ,  B01F13/00 ,  B01J19/00 ,  G01N21/05 ,  G01N21/64 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  C12N15/10 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N15/14 ,  B01L9/00 ,  B01L7/00 ,  G01N21/03 ,  G01N35/00

CPC分类号： B01F3/0807 ,  B01F13/0062 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502784 ,  B01L3/565 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  C12N15/1068 ,  C12N15/1086 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N15/147 ,  G01N21/05 ,  G01N21/64 ,  G01N27/3275 ,  G01N35/08 ,  G01N2021/0346 ,  G01N2035/00237 ,  G01N2035/00326 ,  G01N2201/024 ,  Y10T137/87571 ,  Y10T137/87619 ,  Y10T137/87652 ,  C12Q2525/191 ,  C12Q2563/159 ,  C12Q2535/101 ,  C12Q2563/149 ,  C12Q2531/113

摘要： The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

公开(公告)号：US10045941B2

公开(公告)日：2018-08-14

申请号：US13786873

申请日：2013-03-06

申请人： Pacira Pharmaceuticals, Inc.

发明人： Ernest George Schutt ,  Ronald Warren McGuire ,  Peter Andrew Walters ,  Kathleen D. A. Los

IPC分类号： A61K9/127 ,  B01D1/16 ,  A61K31/4458 ,  B01F3/08 ,  B01F5/10 ,  B01F7/00 ,  A61K9/48 ,  A61M11/00 ,  B01J13/04 ,  B01J13/12 ,  A61K31/445 ,  B01F3/04 ,  B01F15/06

CPC分类号： A61K9/1271 ,  A61K9/127 ,  A61K9/1277 ,  A61K9/4833 ,  A61K31/445 ,  A61K31/4458 ,  A61M11/00 ,  B01D1/16 ,  B01F3/04049 ,  B01F3/0807 ,  B01F3/088 ,  B01F5/104 ,  B01F7/0075 ,  B01F15/065 ,  B01F2015/061 ,  B01F2215/0032 ,  B01J13/043 ,  B01J13/125

摘要： The present invention generally relates to the field of pharmaceutical sciences. More specifically, the present invention includes apparatus and devices for the preparation of pharmaceutical formulations containing large diameter synthetic membrane vesicles, such as multivesicular liposomes, methods for preparing such formulations, and the use of specific formulations for therapeutic treatment of subjects in need thereof. Formation and use of the pharmaceutical formulations containing large diameter synthetic membrane vesicles produced by using the apparatus and devices for therapeutic treatment of subjects in need thereof is also contemplated.

公开(公告)号：US20180178176A1

公开(公告)日：2018-06-28

申请号：US15738713

申请日：2016-06-30

申请人： Sumitomo Heavy Industries Process Equipment Co., Ltd. ,  IZUMI FOOD MACHINERY Co., Ltd.

发明人： Shoji Morinaga ,  Katsuhide Takenaka ,  Keiji Esaki ,  Tetsuya Miyata ,  Soushi Murakami ,  Daisuke Sugifune

IPC分类号： B01F7/22 ,  B01F3/08 ,  B01F3/10 ,  B01F7/00 ,  B01F15/06

CPC分类号： B01F7/22 ,  B01F3/08 ,  B01F3/0807 ,  B01F3/0853 ,  B01F3/10 ,  B01F7/00341 ,  B01F7/00441 ,  B01F7/006 ,  B01F7/166 ,  B01F15/065 ,  B01F2015/061 ,  B01F2015/062

摘要： Provided is a stirring device including: a stirred tank, of which an inner peripheral wall has a circular shape in cross section; and at least one flow impeller and at least one shearing impeller that are located inside the stirred tank and configured to be rotatable independently of each other, in which rotational centers of the flow impeller and the shearing impeller are coaxially provided, the flow impeller rotates around a vertical axis along the inner peripheral wall of the stirred tank to form at least a flow directed toward a lower side in the stirring object existing in the stirred tank, and the shearing impeller imparts a shearing force to the stirring object and is provided on a radially inward side of the flow impeller in the stirred tank and at a position contacting the flow of the stirring object formed by the flow impeller.

公开(公告)号：US20180178174A1

公开(公告)日：2018-06-28

申请号：US15886212

申请日：2018-02-01

申请人： Raindance Technologies, Inc.

发明人： Darren Roy Link ,  Michael Weiner ,  David Marran ,  Jonathan M. Rothberg

IPC分类号： B01F3/08 ,  G01N35/08 ,  G01N27/327 ,  B01F13/00 ,  B01J19/00 ,  G01N21/05 ,  G01N21/64 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  C12N15/10 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N15/14 ,  B01L9/00 ,  B01L7/00 ,  G01N21/03 ,  G01N35/00

CPC分类号： B01F3/0807 ,  B01F13/0062 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502784 ,  B01L3/565 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  C12N15/1068 ,  C12N15/1086 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N15/147 ,  G01N21/05 ,  G01N21/64 ,  G01N27/3275 ,  G01N35/08 ,  G01N2021/0346 ,  G01N2035/00237 ,  G01N2035/00326 ,  G01N2201/024 ,  Y10T137/87571 ,  Y10T137/87619 ,  Y10T137/87652 ,  C12Q2525/191 ,  C12Q2563/159 ,  C12Q2535/101 ,  C12Q2563/149 ,  C12Q2531/113

摘要： The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

公开(公告)号：US20180171373A1

公开(公告)日：2018-06-21

申请号：US15884215

申请日：2018-01-30

申请人： President and Fellows of Harvard College

发明人： David A. Weitz ,  Jeremy Agresti ,  Liang-Yin Chu ,  Jin-Woong Kim ,  Amy Rowat ,  Morten Sommer ,  Gautam Dantas ,  George Church

IPC分类号： C12P19/34 ,  B01F3/08 ,  B01J13/00 ,  C12Q1/6834 ,  B01F13/00

CPC分类号： C12P19/34 ,  B01F3/0807 ,  B01F3/0811 ,  B01F13/0062 ,  B01F13/0071 ,  B01F2215/0037 ,  B01J13/0052 ,  B01J13/0065 ,  B01L3/502784 ,  C12Q1/6834 ,  C12Q1/6848 ,  C12Q1/686 ,  G01N15/1404 ,  G01N2015/1006

摘要： The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.