公开(公告)号：US11351510B2

公开(公告)日：2022-06-07

申请号：US15996253

申请日：2018-06-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Michael Weiner ,  David Marran ,  Jonathan M. Rothberg

IPC: B01F23/41 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6874 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12N15/10 ,  B01J19/00 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  C12Q1/00 ,  G01N15/14 ,  G01N21/05 ,  G01N21/64 ,  G01N35/08 ,  B01F33/3011 ,  G01N27/327 ,  B01L7/00 ,  B01L9/00 ,  G01N35/00 ,  G01N21/03

Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

公开(公告)号：US12146134B2

公开(公告)日：2024-11-19

申请号：US15996222

申请日：2018-06-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC: C12N15/10 ,  B01F33/302 ,  B01F33/3031 ,  B01J19/00 ,  B01L3/00 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/04 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6876 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N33/536 ,  G01N33/543 ,  G01N33/68 ,  B01F23/41 ,  B01L7/00 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/6869 ,  G01N21/64 ,  G01N33/542

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.