公开(公告)号：US20240336912A1

公开(公告)日：2024-10-10

申请号：US18279440

申请日：2022-03-04

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Sasha Levy ,  Xianan LIU ,  Takeshi Matsui ,  Darach Miller ,  Weiyi LI

IPC: C12N15/10 ,  C12N15/11 ,  C12N15/66 ,  C40B50/06

CPC classification number: C12N15/1031 ,  C12N15/11 ,  C12N15/66 ,  C40B50/06

Abstract: Provided herein, inter alia, are methods and compositions for assembling oligonucleotide fragments in vivo. The methods bypass inefficient cloning methods and the requirement for expensive enzymes. The methods may further be used to assemble long fragments of DNA. The methods may be used to generate variant libraries and combinatorial libraries and may be used to trace biological processes. Also provided herein, inter alia, are methods for in vivo DNA barcoding of oligonucleotide sequences. Methods provided herein are contemplated to produce unique barcode-oligonucleotide fusion sequences for identifying and isolating the oligonucleotide sequences, e.g., from a mixture. Accordingly, also provided are methods of identifying an oligonucleotide from a mixture of oligonucleotides.

公开(公告)号：US20240317835A1

公开(公告)日：2024-09-26

申请号：US18733576

申请日：2024-06-04

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal ,  Shunhai Wang ,  Ning Li

IPC: C07K14/71 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/02 ,  G01N30/80

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US12049489B2

公开(公告)日：2024-07-30

申请号：US18340707

申请日：2023-06-23

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal ,  Shunhai Wang ,  Ning Li

IPC: C07K1/36 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K14/71 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  G01N30/02

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to methods for producing aflibercept from a host cell cultured in a chemically defined medium (CDM) including purification of aflibercept, wherein aflibercept following purification includes aflibercept variants that have at least one oxidized amino acid residue selected from the group consisting of tryptophan, histidine and a combination thereof.

公开(公告)号：US12037615B2

公开(公告)日：2024-07-16

申请号：US17079097

申请日：2020-10-23

Applicant: INTEGRATED DNA TECHNOLOGIES, INC.

Inventor： Jessica Woodley ,  Bernice Thommandru ,  Joseph Dobosy ,  Mark Behlke ,  Adam Clore ,  Garrett Rettig ,  Beimeng Sun

IPC: C12N15/66 ,  C12N9/22 ,  C12N15/113

CPC classification number: C12N9/22 ,  C12N15/113 ,  C12N15/66 ,  C12N2310/122 ,  C12N2310/20 ,  C12N2310/321 ,  C12N2310/531 ,  C12N2800/80

Abstract: Described herein are compositions and methods for improving homology directed repair (HDR) efficiency and reducing homology-independent integration following introduction of double strand breaks with engineered nucleases. Additionally, modifications to double stranded DNA donors to improve the donor potency and efficiency of homology directed repair following introduction of double stranded breaks with programmable nucleases.

公开(公告)号：US20240226262A9

公开(公告)日：2024-07-11

申请号：US18467682

申请日：2023-09-14

Applicant: Prime Bio, Inc.

Inventor： Bal Ram Singh

IPC: A61K39/02 ,  A61K39/00 ,  A61K39/08 ,  A61K39/395 ,  A61P31/04 ,  C07K14/19 ,  C07K14/235 ,  C07K14/33 ,  C07K14/34 ,  C12N15/62 ,  C12N15/66 ,  C12N15/70

CPC classification number: A61K39/099 ,  A61K39/0018 ,  A61K39/08 ,  A61K39/39525 ,  A61P31/04 ,  C07K14/19 ,  C07K14/235 ,  C07K14/33 ,  C07K14/34 ,  C12N15/62 ,  C12N15/66 ,  C12N15/70 ,  A61K2039/542 ,  A61K2039/6031 ,  A61K2039/622

Abstract: The present invention describes a second-generation tetanus toxoid vaccine and a process for the preparation thereof, comprising the steps of: inducing an E. Coli culture OD 600=0.5 by adding 0.2 mM IPTG; growing the culture at 14-16° C. for 14 to 20 hours; suspending the culture in 25 mM phosphate buffer containing 200 mM sodium chloride; adding 1% of triton-X-100 to the phosphate buffer, and adding the buffer to the culture; sonicating the culture for a period of 3 minutes (at 5 sec on/off pulse) at 4° C. on cold beads; centrifuging the culture for 60 to 90 minutes; collecting and purifying a supernatant using Ni-NTA affinity column with an eluant; and combining the supernatant into a pool with contaminated bands and concentrating using Centriprep-30 centrifuge filters (30 kDa pores).

公开(公告)号：US20240174998A1

公开(公告)日：2024-05-30

申请号：US18422843

申请日：2024-01-25

Applicant: Caribou Biosciences, Inc.

Inventor： Stan Johan Josef Brouns ,  John van der Oast

IPC: C12N9/22 ,  A61K48/00 ,  C07K14/245 ,  C07K14/47 ,  C12N9/16 ,  C12N15/62 ,  C12N15/66 ,  C12N15/70 ,  C12N15/74 ,  C12N15/81 ,  C12N15/82 ,  C12N15/86 ,  C12N15/90 ,  A61K38/00

CPC classification number: C12N9/22 ,  A61K48/005 ,  C07K14/245 ,  C07K14/47 ,  C12N9/16 ,  C12N15/62 ,  C12N15/66 ,  C12N15/70 ,  C12N15/74 ,  C12N15/81 ,  C12N15/82 ,  C12N15/86 ,  C12N15/902 ,  C12N15/907 ,  A61K38/00 ,  C07K2319/09 ,  C07K2319/22 ,  C07K2319/60 ,  C07K2319/71 ,  C07K2319/80 ,  C07K2319/85 ,  C12N2310/20 ,  C12Y301/21004

Abstract: A clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for adaptive antiviral defence (Cascade); the Cascade protein complex comprising at least CRISPR-associated protein subunits Cas7, Cas5 and Cas6 which includes at least one subunit with an additional amino acid sequence possessing nucleic acid or chromatin modifying, visualising, transcription activating or transcription repressing activity. The Cascade complex with additional activity is combined with an RNA molecule to produce a ribonucleoprotein complex. The RNA molecule is selected to have substantial complementarity to a target sequence. Targeted ribonucleoproteins can be used as genetic engineering tools for precise cutting of nucleic acids in homologous recombination, non-homologous end joining, gene modification, gene integration, mutation repair or for their visualisation, transcriptional activation or repression. A pair of ribonucleotides fused to FokI dimers may be used to generate double-strand breakages in the DNA to facilitate these applications in a sequence-specific manner.

公开(公告)号：US20240174748A1

公开(公告)日：2024-05-30

申请号：US18432126

申请日：2024-02-05

Applicant: INNATE PHARMA

Inventor： LAURENT GAUTHIER ,  NADIA ANCERIZ ,  ARIANE MOREL ,  BENJAMIN ROSSI

IPC: C07K16/28 ,  C07K16/46 ,  C12N5/10 ,  C12N15/00 ,  C12N15/64 ,  C12N15/66

CPC classification number: C07K16/2803 ,  C07K16/2809 ,  C07K16/2887 ,  C07K16/468 ,  C12N5/10 ,  C12N15/00 ,  C12N15/64 ,  C12N15/66 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/526 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2317/92 ,  C12N2511/00 ,  C12N2523/00

Abstract: The application pertains to NKp46-binding immunoglobulin variable regions, and proteins such as antibodies and multispecific proteins that comprise the variable regions. The proteins can bind and specifically redirect NK cells to lyse a target cell of interest. The proteins have utility in the treatment of disease, notably cancer or infectious disease.

公开(公告)号：US11981892B2

公开(公告)日：2024-05-14

申请号：US16384612

申请日：2019-04-15

Applicant: UNIVERSITY OF MASSACHUSETTS

Inventor： Craig Cameron Mello ,  Krishna Sumanth Ghanta ,  Gregoriy Dokshin ,  Aamir Mir ,  Hassan Gneid ,  Jonathan Kenneth Watts ,  Erik Joseph Sontheimer

IPC: C12N15/10 ,  C12N9/22 ,  C12N15/113 ,  C12N15/66 ,  C12N15/90

CPC classification number: C12N15/102 ,  C12N9/22 ,  C12N15/113 ,  C12N15/66 ,  C12N15/902

Abstract: The disclosure provides novel methods and compositions for gene editing. In particular, the disclosure relates to compositions and methods of making modified nucleic acid donor templates for highly efficient and precise gene editing.

公开(公告)号：US11932858B2

公开(公告)日：2024-03-19

申请号：US16772574

申请日：2018-12-14

Applicant: DONALD DANFORTH PLANT SCIENCE CENTER

Inventor： Blake Meyers ,  Rebecca Bart ,  Kira Veley ,  Ihuoma Okwuonu

IPC: C12N15/66 ,  C12N15/115

CPC classification number: C12N15/66 ,  C12N15/115 ,  C12N2310/20 ,  C12Q2521/301

Abstract: Compositions and methods for efficiently generating and identifying accurate homologous recombination events are disclosed.

公开(公告)号：US11845054B2

公开(公告)日：2023-12-19

申请号：US17378133

申请日：2021-07-16

Applicant: Gen9, Inc.

Inventor： Joseph Jacobson ,  Li-Yun A. Kung ,  Andrew Kirk Wilson ,  Senthil Ramu ,  Daniel Schindler ,  Michael E. Hudson

IPC: C12Q1/68 ,  B01J19/00 ,  C12N15/10 ,  C12N15/66 ,  C12P19/34

CPC classification number: B01J19/0046 ,  C12N15/10 ,  C12N15/1031 ,  C12N15/66 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12P19/34 ,  C12N15/10 ,  C12Q2521/343 ,  C12Q2521/50 ,  C12N15/10 ,  C12Q2521/50 ,  C12Q2521/501 ,  C12N15/10 ,  C12N15/64

Abstract: Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.