公开(公告)号：US20240318163A1

公开(公告)日：2024-09-26

申请号：US18576490

申请日：2022-06-15

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Fumio Maeda ,  Shungo Adachi ,  Tohru Natsume

IPC: C12N15/10

CPC classification number: C12N15/1006 ,  C12N15/1017

Abstract: A method for noninvasively acquiring a microRNA from a cell, the method comprising (1) introducing a nucleic acid coding a microRNA-binding protein and a nucleic acid coding a vesicle-forming protein into the cell, wherein the microRNA-binding protein comprises a first portion consisting of an MID domain and a PIWI domain of an Argonaute protein and a second portion consisting of a virus protein R, and the vesicle-forming protein comprises: a palmitoylation or myristoylation signal or a PH domain; a self-assembling domain; an ESCRT or ESCRT-related factor-binding domain; and a Gag p6 domain; thereby producing an exosome-like vesicle comprising a micro-RNA, (2) collecting an extracellular fluid of the cell, and (3) extracting the micro-RNA from the extracellular fluid.

公开(公告)号：US20240301396A1

公开(公告)日：2024-09-12

申请号：US18671062

申请日：2024-05-22

Applicant: QIAGEN GmbH

Inventor： Alexander Wolf ,  Sandra Hammerschmidt ,  Thorsten Voss

IPC: C12N15/10 ,  C12Q1/6806

CPC classification number: C12N15/101 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

Abstract: The present invention pertains to methods and kits for isolating extracellular nucleic acids from a biological sample using anion exchange particles. It was found that incorporating into the binding mixture a polyoxyalkylene fatty alcohol ether compensates performance variations that are attributable to differences in the anion exchange surface as they may occur e.g. between different lots/batches of the anion exchange particles and/or during storage of said particles. Moreover, including a polyoxyalkylene fatty alcohol ether in the binding mixture resulted in a higher purity of the obtained eluates revealing significantly less inhibition in a downstream reaction such as a PCR reaction.

公开(公告)号：US12084650B2

公开(公告)日：2024-09-10

申请号：US18136961

申请日：2023-04-20

Applicant: Norgen Biotek Corp.

Inventor： Taha Alexander Haj-Ahmad ,  Yousef Haj-Ahmad

IPC: C12Q1/6806 ,  C11D1/08 ,  C11D1/10 ,  C11D3/04 ,  C11D3/37 ,  C12N1/04 ,  C12N15/10 ,  C12N9/99

CPC classification number: C12N15/1006 ,  C11D1/08 ,  C11D1/10 ,  C11D3/046 ,  C11D3/37 ,  C12N1/04 ,  C12Q1/6806 ,  C12N9/99 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2527/127

Abstract: Provided is a composition for preserving cell-free nucleic acids and/or cells in a biological sample and methods for use thereof. The composition comprises at least one volume excluding polymer, at least one osmotic agent and at least one enzyme inhibitor. The composition optionally further comprises at least one metabolic inhibitor. Further, provided is a kit comprising the composition, preferably in a blood collection tube, or the components of the composition.

公开(公告)号：US12044687B1

公开(公告)日：2024-07-23

申请号：US16818441

申请日：2020-03-13

Applicant: NantBio, Inc.

Inventor： Clifford Anders Olson ,  Kayyan Niazi ,  Nicholas J. Witchey ,  Wael Tadros

IPC: G01N33/68 ,  C12N5/10 ,  C12N15/10 ,  C12Q1/6897 ,  G01N21/64 ,  G01N33/50

CPC classification number: G01N33/6872 ,  C12N5/10 ,  C12N15/1006 ,  C12Q1/6897 ,  G01N21/64 ,  G01N33/5091 ,  G01N2333/705

Abstract: The present disclosure relates to recombinant production reporter cells comprising a logic gate such that an expressible sequence (e.g., a reporter gene) is expressed after the occurrence of triggering event related to a stage of production or an environmental condition of a biological production system. Nucleic acids, kits, and methods for making and using the recombinant production reporter cells are also disclosed herein.

公开(公告)号：US12038360B2

公开(公告)日：2024-07-16

申请号：US17289282

申请日：2019-10-30

Applicant: CRAIF INC.

Inventor： Takao Yasui ,  Yoshinobu Baba ,  Hirotaka Koga

IPC: G01N1/40 ,  B82Y5/00 ,  B82Y35/00 ,  B82Y40/00 ,  C12N15/10 ,  D04H1/425

CPC classification number: G01N1/4077 ,  C12N15/1006 ,  D04H1/425 ,  B82Y5/00 ,  B82Y35/00 ,  B82Y40/00 ,  D10B2201/01 ,  G01N2001/4088

Abstract: The present invention provides a novel miRNA extraction method and a method for analyzing miRNA extracted by using said miRNA extraction method. According to the present invention, provided is, for example, a method for extracting miRNA from extracellular vesicles in a sample solution, by using a device capable of capturing extracellular vesicles, the miRNA extraction method comprising: an extracellular vesicle capturing step for capturing extracellular vesicles in a sample solution onto a device by bringing the sample solution and the device in contact with each other; and a miRNA extraction step for homogenizing the extracellular vesicles by bringing the device having captured the extracellular vesicles in contact with a homogenization liquid for extracellular vesicles to extract miRNA from the extracellular vesicle into the homogenization liquid.

公开(公告)号：US11995558B2

公开(公告)日：2024-05-28

申请号：US16415666

申请日：2019-05-17

Applicant: The Charles Stark Draper Laboratory, Inc.

Inventor： Kirsty A. McFarland ,  Andrew P. Magyar ,  Ian Ward Frank ,  Kenneth T. Kotz ,  Stacey Markovic ,  Haiyao Huang ,  Steven J. Byrnes ,  Gregg E. Favalora ,  Melissa M. Sprachman ,  Ryan A. Dubay ,  Emma Vargo ,  Peter Cavanagh ,  Erin Rosenberger

IPC: G06N3/12 ,  B01L3/00 ,  B81B1/00 ,  C12N9/12 ,  C12N15/10 ,  C12P19/34 ,  G06N3/123 ,  G11C13/02

CPC classification number: G06N3/123 ,  B01L3/5027 ,  B01L3/502761 ,  B01L3/50851 ,  B01L3/527 ,  B81B1/006 ,  C12N9/12 ,  C12N15/1006 ,  C12N15/1065 ,  C12N15/1068 ,  C12P19/34 ,  G11C13/02 ,  B01J2219/00495

Abstract: A parallelized chain-synthesizing technique includes capillary tubes, where each tube provides multiple locations or addresses where a specific arbitrary sequence for polymeric chains can be synthesized. An optical addressing system selectively delivers light to the locations to mediate or control reactions in the tubes.

公开(公告)号：US20240167088A1

公开(公告)日：2024-05-23

申请号：US18499663

申请日：2023-11-01

Applicant: esBiolab LLC

Inventor： Liangliang Qiang ,  Wei Li ,  Aijie Han

IPC: C12Q1/6874 ,  B01L3/00 ,  C12N9/16 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/686

CPC classification number: C12Q1/6874 ,  B01L3/502753 ,  C12N9/16 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Y301/11

Abstract: The present disclosure describes methods and microfluidic devices for generating clusters of amplicons for a nucleic acid library, and their uses for high-throughput DNA sequencing or detection of target polynucleotides in a sample.

公开(公告)号：US20240141415A1

公开(公告)日：2024-05-02

申请号：US18095982

申请日：2023-01-11

Applicant: Cepheid

Inventor： Edwin Wei-Lung Lai ,  Andrew Kohlway ,  Reuel Van Atta ,  Russell Higuchi ,  Alexander A. Gall ,  Kriszten Kocmond

IPC: C12Q1/6827 ,  B01L7/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6886

CPC classification number: C12Q1/6827 ,  B01L7/52 ,  C12N15/1006 ,  C12N15/1017 ,  C12Q1/6806 ,  C12Q1/6886 ,  B01L2300/0864 ,  B01L2300/087 ,  C12Q2600/154

Abstract: In various embodiments methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulfonating the bound deaminated nucleic acid and/or simultaneously eluting and desulfonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.

公开(公告)号：US11952615B2

公开(公告)日：2024-04-09

申请号：US17529896

申请日：2021-11-18

Applicant: Exact Sciences Corporation

Inventor： Hatim T. Allawi ,  Graham P. Lidgard ,  Brian Aizenstein ,  Melissa M. Gray

IPC: C12Q1/6806 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12Q2600/154 ,  C12Q2600/16 ,  C12Q1/6806 ,  C12Q2523/125 ,  C12Q2545/101

Abstract: The present invention provides synthetic DNA strands that find use as process controls in DNA processing and nucleic acid testing methods. In particular, provided herein are synthetic methylated DNA strands of known composition for use as control molecules in DNA testing, e.g., of mutations and/or methylation of DNA isolated from non-fish samples, such as human samples.

公开(公告)号：US11866695B2

公开(公告)日：2024-01-09

申请号：US17131449

申请日：2020-12-22

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Erik Jue ,  Daan Witters

IPC: C12N15/10 ,  C12Q1/6806

CPC classification number: C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

Abstract: Methods and systems and related compositions for separating through a solid matrix a mixture comprising a nucleic acid in a concentration of 1 μM or less, together with a target compound having a water solubility equal to or greater than 0.001 g per 100 mL, which can be used for managing fluid flow, biochemical reactions and purification of the nucleic acid or other target analytes. The method comprises contacting the solid matrix with a target compound removing agent having a water solubility equal to or less than 0.05 g per 100 mL at 25° C. at 1 atm pressure with water having a solubility in the removing agent of less than 10 g per 100 mL at 25° C. at 1 atm pressure, the contacting performed to remove the target compound from the solid matrix.