公开(公告)号：US20250066858A1

公开(公告)日：2025-02-27

申请号：US18805191

申请日：2024-08-14

Applicant: Exact Sciences Corporation

Inventor： Hatim Allawi ,  Graham P. Lidgard ,  Chateen Krueger ,  Michael W. Kaiser ,  Tamara J. Sander

IPC: C12Q1/6886

Abstract: Provided herein is technology for lung neoplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of lung cancer.

公开(公告)号：US20250034653A1

公开(公告)日：2025-01-30

申请号：US18919170

申请日：2024-10-17

Applicant: Exact Sciences Corporation

Inventor： Joost Louwagie

IPC: C12Q1/6886 ,  G01N33/574

Abstract: Methods and kits directed to processing a fresh fecal sample from a human subject by a process comprising removing a portion of a collected fecal sample and adding the removed portion of the sample to a buffer that prevents denaturation or degradation of blood proteins, and stabilizing the remaining portion of the fecal sample with a stabilizing buffer that maintains nucleic acid integrity.

公开(公告)号：US20250027158A1

公开(公告)日：2025-01-23

申请号：US18744995

申请日：2024-06-17

Applicant: Exact Sciences Corporation ,  Mayo Foundation for Medical Education and Research

Inventor： Hatim T. Allawi ,  Michael W. Kaiser ,  Graham P. Lidgard ,  William R. Taylor ,  Tamara J. Sander ,  Abram M. Vaccaro

IPC: C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6853 ,  C12Q1/6886 ,  G01N33/533 ,  G01N33/574 ,  G16Z99/00

Abstract: Provided herein is technology for neoplasia screening, and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of cancer, in particular, colorectal cancer.

公开(公告)号：US20240417810A1

公开(公告)日：2024-12-19

申请号：US18759437

申请日：2024-06-28

Applicant: Exact Sciences Corporation

Inventor： Rebecca Oldham-Haltom ,  Hatim T. Allawi ,  Hongzhi Zou ,  Michael J. Domanico ,  Graham P. Lidgard

IPC: C12Q1/6886 ,  C12Q1/6858

Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

公开(公告)号：US20240407766A1

公开(公告)日：2024-12-12

申请号：US18655072

申请日：2024-05-03

Applicant: Exact Sciences Corporation

Inventor： Graham P. Lidgard ,  Michael J. Domanico ,  Keith Fourrier ,  James P. Light, II ,  Scott Castanon ,  Keith Kopitzke

IPC: A61B10/00 ,  B01L3/00 ,  G01N1/38

Abstract: Provided herein is technology relating to collecting and preparing samples. For example, the technology relates particularly, but not exclusively, to devices, systems, and kits that allow a subject to collect and prepare a sample for analysis.

公开(公告)号：US12060620B2

公开(公告)日：2024-08-13

申请号：US17739892

申请日：2022-05-09

Applicant: Exact Sciences Corporation

Inventor： Rebecca Oldham-Haltom ,  Hatim Allawi ,  Hongzhi Zou ,  Michael J. Domanico ,  Graham P. Lidgard

IPC: C12Q1/68 ,  C12Q1/6858 ,  C12Q1/6886

CPC classification number: C12Q1/6886 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q1/6858 ,  C12Q2525/161 ,  C12Q2561/109 ,  C12Q2565/1015

Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

公开(公告)号：US20230407407A1

公开(公告)日：2023-12-21

申请号：US18316883

申请日：2023-05-12

Applicant: Exact Sciences Corporation

Inventor： Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi ,  Hongzhi Zou

IPC: C12Q1/6886 ,  C12Q1/686 ,  C12Q1/6827

CPC classification number: C12Q1/6886 ,  C12Q1/686 ,  C12Q1/6827 ,  C12Q2600/154

Abstract: A method for detecting a methylated genomic locus is provided. In certain embodiments, the method comprises: a) treating a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an agent that modifies cytosine to uracil to produce a treated nucleic acid; b) amplifying a product from the treated nucleic acid using a first primer and a second primer, wherein the first primer hybridizes to a site in the locus that contain methylcytosines and the amplifying preferentially amplifies the methylated copies of the genomic locus, to produce an amplified sample; and c) detecting the presence of amplified methylated copies of the genomic locus in the amplified sample using a flap assay that employs an invasive oligonucleotide having a 3′ terminal G or C nucleotide that corresponds to a site of methylation in the genomic locus.

公开(公告)号：US11845991B2

公开(公告)日：2023-12-19

申请号：US15634607

申请日：2017-06-27

Applicant: Exact Sciences Corporation

Inventor： Joost Louwagie

IPC: C12Q1/6886 ,  G01N33/574

CPC classification number: C12Q1/6886 ,  G01N33/57446 ,  C12Q2600/106 ,  C12Q2600/154 ,  C12Q2600/158 ,  G01N2333/805 ,  G01N2800/50 ,  G01N2800/52

Abstract: A method of processing a fecal sample from a human subject comprising removing a portion of a collected fecal sample and adding the removed portion of the sample to a buffer that prevents denaturation or degradation of blood proteins found in the sample, and detecting the presence of human blood in the removed portion of the fecal sample. The method further comprises stabilizing the remaining portion of the fecal sample.

公开(公告)号：US20230392189A1

公开(公告)日：2023-12-07

申请号：US18310276

申请日：2023-05-01

Applicant: Exact Sciences Corporation

Inventor： Hatim Allawi ,  Graham P. Lidgard ,  Brian Aizenstein ,  Tamara J. Sander ,  Maria Giakoumopoulos ,  Michael W. Kaiser ,  Melissa M. Gray ,  Abram Michael Vaccaro

IPC: C12Q1/6806 ,  C12Q1/686 ,  C12N15/10 ,  C12Q1/6837

CPC classification number: C12Q1/6806 ,  C12Q1/686 ,  C12N15/1006 ,  C12Q1/6837 ,  C12Q2600/154 ,  C12Q2600/166

Abstract: Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

公开(公告)号：US20230313317A1

公开(公告)日：2023-10-05

申请号：US18179945

申请日：2023-03-07

Applicant: Exact Sciences Corporation

Inventor： Joost Louwagie

IPC: C12Q1/6886 ,  G01N33/574

CPC classification number: C12Q1/6886 ,  G01N33/57446 ,  C12Q2600/106 ,  C12Q2600/154 ,  C12Q2600/158 ,  G01N2333/805 ,  G01N2800/50 ,  G01N2800/52

Abstract: A method of processing a fecal sample from a human subject comprising combining a first portion of a collected fecal sample with a stabilizing buffer, combining a second portion of the sample with a solution that prevents denaturation or degradation of blood proteins found in a fecal sample. Embodiments comprise testing nucleic acid extracted from the first portion of the fecal sample for an amount of a human nucleic acid, and testing the second portion of the fecal sample for the presence of human blood.