公开(公告)号：US11708603B2

公开(公告)日：2023-07-25

申请号：US17174127

申请日：2021-02-11

Applicant: Exact Sciences Corporation

Inventor： Hatim Allawi ,  Rebecca Oldham-Haltom ,  Zubin Gagrat ,  Michael Domanico ,  Graham Lidgard

IPC: C12Q1/686 ,  C12Q1/6851

CPC classification number: C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6851 ,  C12Q2521/101 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2549/125

Abstract: Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.

公开(公告)号：US20240417810A1

公开(公告)日：2024-12-19

申请号：US18759437

申请日：2024-06-28

Applicant: Exact Sciences Corporation

Inventor： Rebecca Oldham-Haltom ,  Hatim T. Allawi ,  Hongzhi Zou ,  Michael J. Domanico ,  Graham P. Lidgard

IPC: C12Q1/6886 ,  C12Q1/6858

Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

公开(公告)号：US12060620B2

公开(公告)日：2024-08-13

申请号：US17739892

申请日：2022-05-09

Applicant: Exact Sciences Corporation

Inventor： Rebecca Oldham-Haltom ,  Hatim Allawi ,  Hongzhi Zou ,  Michael J. Domanico ,  Graham P. Lidgard

IPC: C12Q1/68 ,  C12Q1/6858 ,  C12Q1/6886

CPC classification number: C12Q1/6886 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q1/6858 ,  C12Q2525/161 ,  C12Q2561/109 ,  C12Q2565/1015

Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

公开(公告)号：US10604793B2

公开(公告)日：2020-03-31

申请号：US15068364

申请日：2016-03-11

Applicant: Exact Sciences Corporation

Inventor： Rebecca Oldham-Haltom ,  Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12Q1/68 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6818 ,  C12Q1/6886

Abstract: A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

公开(公告)号：US09290797B2

公开(公告)日：2016-03-22

申请号：US13720757

申请日：2012-12-19

Applicant: Exact Sciences Corporation

Inventor： Rebecca Oldham-Haltom ,  Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6818 ,  C12Q1/686 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2525/301 ,  C12Q2527/101 ,  C12Q2561/109 ,  C12Q2565/1015

Abstract: A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

公开(公告)号：US20140087382A1

公开(公告)日：2014-03-27

申请号：US14036649

申请日：2013-09-25

Applicant: Exact Sciences Corporation

Inventor： Hatim Allawi ,  Rebecca Oldham-Haltom ,  Zubin Gagrat ,  Michael Domanico ,  Graham Lidgard

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6851 ,  C12Q2521/101 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2549/125

Abstract: Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.

Abstract translation: 本文提供了与核酸的基于扩增的检测有关的技术，特别但并非排他地涉及用于最小化不同样品或制造大量DNA聚合酶如Taq DNA聚合酶之间的活性的变异性的方法和组合物。

公开(公告)号：US20160237480A1

公开(公告)日：2016-08-18

申请号：US15019758

申请日：2016-02-09

Applicant: Exact Sciences Corporation

Inventor： Rebecca Oldham-Haltom ,  Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6818 ,  C12Q1/686 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2525/301 ,  C12Q2527/101 ,  C12Q2561/109 ,  C12Q2565/1015

Abstract: A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

公开(公告)号：US20160010161A1

公开(公告)日：2016-01-14

申请号：US14811266

申请日：2015-07-28

Applicant: Exact Sciences Corporation

Inventor： Rebecca Oldham-Haltom ,  Hatim Allawi ,  Hongzhi Zou ,  Michael J. Domanico ,  Graham P. Lidgard

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2525/161 ,  C12Q2561/109 ,  C12Q2565/1015

Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

Abstract translation: 本文提供的试剂混合物包含多重扩增试剂和皮瓣测定试剂，用于在单次反应中检测含有34A，34C，34T，35A，35C，35T或38A点突变中任何一种的KRAS基因的突变体拷贝。 还提供了使用试剂混合物和试剂盒进行其的方法。

公开(公告)号：US20240409988A1

公开(公告)日：2024-12-12

申请号：US18330105

申请日：2023-06-06

Applicant: Exact Sciences Corporation

Inventor： Hatim Allawi ,  Rebecca Oldham-Haltom ,  Zubin Gagrat ,  Michael Domanico ,  Graham Lidgard

IPC: C12Q1/6851

Abstract: Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.

公开(公告)号：US20180066319A1

公开(公告)日：2018-03-08

申请号：US15686065

申请日：2017-08-24

Applicant: Exact Sciences Corporation

Inventor： Rebecca Oldham-Haltom ,  Hatim Allawi ,  Hongzhi Zou ,  Michael J. Domanico ,  Graham P. Lidgard

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2525/161 ,  C12Q2561/109 ,  C12Q2565/1015

Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.