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    Control for diagnostic assay
    2.
    发明授权
    Control for diagnostic assay 有权

    公开(公告)号：US09822404B2

    公开(公告)日：2017-11-21

    申请号：US14441112

    申请日：2013-11-07

    申请人： QIAGEN GMBH

    发明人： Gerd Grosshauser ,  Andy Wende ,  Ralf Himmelreich

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q2527/125 ,  C12Q2549/125

    摘要： A method for determining whether a lysate contains sufficient biological sample material for a nucleic acid amplification reaction that includes preparing the lysate in the presence of at least one compound that inhibits the amplification reaction if insufficient biological sample material was present during preparation of the lysate, but does not inhibit the amplification reaction if sufficient biological sample material was present during preparation of the lysate, subjecting the lysate to the amplification reaction, and analyzing a result of the amplification reaction. Due to the presence of the compound in the amplification reaction, no amplification signal is obtained if insufficient biological sample material was present during preparation of the lysate but an amplification signal is obtained if sufficient biological sample material was present during preparation of the lysate. The invention allows for reliable identification of false negatives that occur because insufficient sample material was subjected to the amplification assay.

    CONTROL FOR DIAGNOSTIC ASSAY
    3.
    发明申请
    CONTROL FOR DIAGNOSTIC ASSAY 有权
    控制诊断测试

    公开(公告)号：US20150292000A1

    公开(公告)日：2015-10-15

    申请号：US14441112

    申请日：2013-11-07

    申请人： QIAGEN GMBH

    发明人： Gerd Grosshauser ,  Andy Wende ,  Rlaf Himmelreich

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q2527/125 ,  C12Q2549/125

    摘要： A method for determining whether a lysate contains sufficient biological sample material for a nucleic acid amplification reaction that includes preparing the lysate in the presence of at least one compound that inhibits the amplification reaction if insufficient biological sample material was present during preparation of the lysate, but does not inhibit the amplification reaction if sufficient biological sample material was present during preparation of the lysate, subjecting the lysate to the amplification reaction, and analyzing a result of the amplification reaction. Due to the presence of the compound in the amplification reaction, no amplification signal is obtained if insufficient biological sample material was present during preparation of the lysate but an amplification signal is obtained if sufficient biological sample material was present during preparation of the lysate. The invention allows for reliable identification of false negatives that occur because insufficient sample material was subjected to the amplification assay.

    摘要翻译： 一种用于确定裂解物是否含有足够的用于核酸扩增反应的生物样本材料的方法，其包括在至少一种抑制扩增反应的化合物的存在下制备裂解物，如果在制备溶胞产物期间存在不足的生物样品材料，则 如果在制备溶胞产物期间存在足够的生物样品材料，使裂解物进行扩增反应并分析扩增反应的结果，则不抑制扩增反应。 由于在扩增反应中存在化合物，如果在制备溶胞产物期间存在不足的生物样品材料，则不能获得扩增信号，但如果在制备溶胞产物期间存在足够的生物样品材料，则获得扩增信号。 本发明允许可靠地鉴定由于不足的样品材料进行扩增测定而发生的假阴性。

    Methods for the reduction of stutter in microsatellite amplification
    4.
    发明授权
    Methods for the reduction of stutter in microsatellite amplification 有权
    减少微卫星放大的口吃的方法

    公开(公告)号：US06780588B2

    公开(公告)日：2004-08-24

    申请号：US09850590

    申请日：2001-05-07

    申请人： Sulekha Rao Coticone ,  Will Bloch

    发明人： Sulekha Rao Coticone ,  Will Bloch

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6848 ,  C12Q2549/125 ,  C12Q2527/137 ,  C12Q2521/101

    摘要： The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of greater than 50%; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of sorbitol effective to reduce stutter relative to the amount of stutter observed in the absence of sorbitol. The invention also provides compositions containing sorbitol, kits for amplifying microsatellites having a G+C content of greater than 50%, and methods of using all of the foregoing.

    摘要翻译： 本发明提供了减少微卫星扩增中的口吃的方法，包括提供包含G + C含量大于50％的微卫星样品的步骤; 使样品与至少一种具有核酸聚合酶活性的酶接触; 并将样品与酶温育足够的时间和足以扩增微卫星的条件; 其中在一定量的山梨糖醇的存在下进行培养，其有效地减少相对于在不存在山梨糖醇时观察到的口吃量的口吃。 本发明还提供含有山梨醇的组合物，用于扩增G + C含量大于50％的微卫星的试剂盒，以及使用上述所有方法。

    Compositions, methods, and kits for amplifying nucleic acids
    5.
    发明授权
    Compositions, methods, and kits for amplifying nucleic acids 有权
    用于扩增核酸的组合物，方法和试剂盒

    公开(公告)号：US08470531B2

    公开(公告)日：2013-06-25

    申请号：US12633759

    申请日：2009-12-08

    申请人： Shoulian Dong ,  Junko Stevens ,  Danny Lee

    发明人： Shoulian Dong ,  Junko Stevens ,  Danny Lee

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6846 ,  C12N15/115 ,  C12N2310/16 ,  C12P19/34 ,  C12Q1/6848 ,  G01N21/6486 ,  C12Q2549/125 ,  C12Q2527/127 ,  C12Q2527/107

    摘要： The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.

    摘要翻译： 本教导涉及用于扩增靶核酸的组合物，方法和试剂盒，同时减少非特异性荧光和不需要的扩增产物，有时称为二级扩增产物或假副产物。 本文公开的酶抑制剂包含核苷酸序列和至少一种猝灭剂。 还提供了包含与酶相关的酶抑制剂的复合物，其中所述酶的至少一种酶活性被抑制。 公开了减少不期望的扩增产物同时扩增靶核酸的方法，还有用于降低非特异性荧光的方法。 还提供了用于加速某些公开方法的执行的套件。

    Hot start nucleic acid amplification
    6.
    发明授权
    Hot start nucleic acid amplification 有权
    热启动核酸扩增

    公开(公告)号：US08404443B2

    公开(公告)日：2013-03-26

    申请号：US13074752

    申请日：2011-03-29

    申请人： Christopher James Kubu ,  Jeannine Catherine Muller-Greven ,  Robert Bruce Moffett

    发明人： Christopher James Kubu ,  Jeannine Catherine Muller-Greven ,  Robert Bruce Moffett

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6848 ,  C12Q1/686 ,  C12Q2549/125 ,  C12Q2533/101 ,  C12Q2522/101 ,  C12Q2549/101

    摘要： Methods and compositions for performing nucleic acid duplication and amplification reactions are provided. A single-stranded nucleic acid binding protein is selected and provided in the reaction mixture which is assembled at a low, nonstringent temperature to include all of the necessary reagents for successful nucleic acid duplication or amplification reactions. By incorporating a single-stranded nucleic acid binding protein into the reaction mixture at low temperature, the generation of nonspecific products such as amplification products is improved despite the reaction mixture having been fully assembled at a nonstringent temperature.

    摘要翻译： 提供了用于进行核酸复制和扩增反应的方法和组合物。 在反应混合物中选择并提供单链核酸结合蛋白，所述反应混合物在低，非收缩温度下组装以包括用于成功的核酸复制或扩增反应的所有必需试剂。 通过在低温下将单链核酸结合蛋白并入反应混合物中，尽管反应混合物已经在非收敛温度下完全组装，但非特异性产物如扩增产物的产生得到改善。

    Compositions and methods for increasing amplification efficiency
    10.
    发明申请
    Compositions and methods for increasing amplification efficiency 审中-公开
    用于增加放大效率的组合物和方法

    公开(公告)号：US20070117114A1

    公开(公告)日：2007-05-24

    申请号：US11481953

    申请日：2006-07-07

    申请人： Ayoub Rashtchian ,  David Schuster

    发明人： Ayoub Rashtchian ,  David Schuster

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N9/99

    CPC分类号： C12Q1/6848 ,  C12N9/1252 ,  C12N9/99 ,  C12Q1/686 ,  C12Q2549/125 ,  C12Q2549/101 ,  C12Q2521/101 ,  C12Q2527/127 ,  C12Q2521/531 ,  C12Q2521/319

    摘要： Compositions and methods are provided that improve the specificity and efficiency of nucleic acid amplification. A binding partner may be bound to a DNA polymerase enzyme where the binding partner substantially inhibits the activity of the polymerase. A second enzyme modifies the binding partner in a manner that relieves the inhibition of the polymerase activity. The activity of the second enzyme may be inhibited in a temperature-sensitive manner such that the second enzyme is active only at elevated temperatures. As a consequence, the polymerase enzyme also is active only when the temperature is elevated.

    摘要翻译： 提供了提高核酸扩增的特异性和效率的组合物和方法。 结合配偶体可以结合DNA聚合酶，其中结合配偶体基本上抑制聚合酶的活性。 第二种酶以减轻聚合酶活性抑制的方式改变结合配偶体。 可以以温度敏感的方式抑制第二种酶的活性，使得第二种酶仅在升高的温度下是有活性的。 因此，仅当温度升高时，聚合酶也是活性的。