公开(公告)号：US20240336651A1

公开(公告)日：2024-10-10

申请号：US18748165

申请日：2024-06-20

Applicant: NATIONAL SUN YAT-SEN UNIVERSITY

Inventor： Hong-Wei Yang ,  Nan-Si Li ,  Ying-Pei Hsu ,  Hao-Han Pang

IPC: C07K2/00 ,  A61K38/16 ,  B82Y40/00 ,  C03C17/32 ,  C07K14/005 ,  G01N21/78 ,  G01N27/414 ,  G01N33/543

CPC classification number: C07K2/00 ,  A61K38/162 ,  C03C17/32 ,  C07K14/005 ,  G01N21/78 ,  G01N27/4145 ,  G01N33/54393 ,  B82Y40/00 ,  C03C2217/70 ,  C07K2319/30 ,  C07K2319/735 ,  C07K2319/74 ,  C12N2795/00023

Abstract: A method for manufacturing a glass-based biosensor is used to solve the problem of the use of a solution containing a strong acid or a strong base or of an oxygen plasma treatment. The method comprises modifying a silicon-containing substrate by an alcohol solution to form negative charges on at least one coupling surface of the silicon-containing substrate. A least one active layer of polymer having positive charges is formed on the at least one surface of the silicon-containing substrate, respectively. Each of the at least one active layer of polymer has a coupling surface and an active surface opposite to the coupling surface, and the at least one active layer of polymer couples to the silicon-containing substrate via the coupling surface. A plurality of capture biomolecules couples to the active surface.

公开(公告)号：US12105097B2

公开(公告)日：2024-10-01

申请号：US17269257

申请日：2020-01-20

Applicant: China Medical University

Inventor： Gregory Tsay ,  Hsin-Yi Peng

IPC: G01N33/68 ,  C07K7/08 ,  G01N33/543

CPC classification number: G01N33/6893 ,  C07K7/08 ,  G01N33/54393 ,  G01N2800/102

Abstract: The present invention relates to a peptide capable of binding to rheumatoid arthritis autoantibodies, which is a consecutive 10-25 amino acid sequence of any one fragment of the group consisting of SEQ ID NO: 3-4, 7-13 or 16-19, wherein the peptide fragment has an epitope that binds to the rheumatoid arthritis autoantibodies. Furthermore, the peptide fragment bound to the rheumatoid arthritis autoantibodies is used for testing rheumatoid arthritis, and according to this use, the present invention provides a method for testing rheumatoid arthritis disease and a test reagent kit used for determining whether a subject to be tested suffers from rheumatoid arthritis disease.

公开(公告)号：US12072332B2

公开(公告)日：2024-08-27

申请号：US16488880

申请日：2018-02-28

Applicant: VIDCARE INNOVATIONS PVT. LTD.

Inventor： Rohan Aggarwal

IPC: G01N33/543 ,  G01N33/53

CPC classification number: G01N33/54393 ,  G01N33/5308

Abstract: Methods and device for quantification of an analyte in a sample are provided. An example has the following steps: the sample is introduced into at least one test split channel (306). The test split channel (306) comprises a test reaction portion (306a). The analyte (410) in the sample is to bind to capture reagents (408) provided in the reaction portion. Analyte (410) bound to the capture reagents (408) is contacted with a reactant solution. The reactant solution comprises a plurality of reagent coated microparticles (412) for binding with the analyte (410). Residual reactant solution comprising unbound microparticles is received. The residual reactant solution is analyzed to quantify the analyte.

公开(公告)号：US20240280573A1

公开(公告)日：2024-08-22

申请号：US18469907

申请日：2023-09-19

Applicant: California Institute of Technology

Inventor： Rong Fan ,  Habib Ahmad ,  James R. Heath

IPC: G01N33/543 ,  B01J19/00 ,  B01L3/00 ,  G01N33/58

CPC classification number: G01N33/54393 ,  B01J19/0046 ,  B01L3/502746 ,  B01L3/502753 ,  G01N33/54366 ,  G01N33/582 ,  B01J2219/00547 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00621 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/5025 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/084 ,  G01N2458/10

Abstract: Arrays and substrates comprising a material, in particular capture agents and/or detectable targets, attached to the substrates along substantially parallel lines forming a barcoded pattern and related methods and systems.

公开(公告)号：US20240255501A1

公开(公告)日：2024-08-01

申请号：US18006645

申请日：2020-07-24

Applicant: CSEM Centre Suisse d'Electronique et de Microtechnique SA - Recherche et Développement

Inventor： Ye TANG ,  Hui CHAI GAO ,  Silvia GENERELLI

IPC: G01N33/543 ,  C08B15/04 ,  C08L1/02

CPC classification number: G01N33/54393 ,  C08B15/04 ,  C08L1/02 ,  G01N33/54388

Abstract: A method is disclosed for the fabrication of a lateral flow test device including a nanocellulose aerogel pad obtained by implementation of steps consisting in: a) providing a hydrogel containing nanocellulose fibers, preferably carboxylic nanocellulose fibers; b) conducting a chemical crosslinking of said carboxylic nanocellulose fibers; c) conducting a lyophilisation of the hydrogel containing the crosslinked carboxylic nanocellulose fibers so as to define a nanocellulose aerogel; and d) compacting and shaping a predefined amount of said nanocellulose aerogel so as to define the nanocellulose aerogel pad.

公开(公告)号：US20240210398A1

公开(公告)日：2024-06-27

申请号：US18088996

申请日：2022-12-27

Applicant: PRINCETON BIOCHEMICALS, INC.

Inventor： Norberto A. Guzman

IPC: G01N33/569 ,  G01N33/543

CPC classification number: G01N33/56983 ,  G01N33/54388 ,  G01N33/54393 ,  G01N2333/165 ,  G01N2469/10

Abstract: A disease detection system including a platform or cartridge to perform testing using a rapid lateral flow chromatographic immunoassay (LFIA) test intended for the qualitative detection of the disease in a sample, such as collected sputum, disrupted by a digestive enzyme and a detergent to release its content and digest its proteins and other polymeric molecules. A method of the present invention includes treating a sample with a digestive enzyme and a detergent to disrupt the collected sample releasing content and digesting proteins of the sample to form a lysed-digested-extracted sample, applying the lysed-digested-extracted sample to a platform or cartridge, performing a rapid lateral flow chromatographic immunoassay (LFIA) test of the lysed-digested-extracted sample using the platform or cartridge and detecting presence or absence of an analyte of interest from the tested lysed-digested-extracted sample.

公开(公告)号：US11953502B2

公开(公告)日：2024-04-09

申请号：US16755035

申请日：2018-10-12

Applicant: E&S HEALTHCARE CO., LTD.

Inventor： Kyong Hoon Suh ,  Dae Joong Kim ,  Young Kim ,  Mi Kyung Kim ,  Jong Hwan Jung ,  Ki Se Lee

IPC: C07K7/08 ,  C07K7/00 ,  C07K7/06 ,  C07K14/00 ,  C07K16/40 ,  G01N33/543 ,  G01N33/574 ,  G01N33/577 ,  G01N33/58

CPC classification number: G01N33/57415 ,  C07K7/08 ,  C07K16/40 ,  G01N33/54393 ,  G01N33/577 ,  G01N33/581 ,  C07K7/00 ,  C07K7/06 ,  C07K14/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/622

Abstract: The present invention relates to an epitope of a thioredoxin-1 (Trx1) antigen and a use thereof, and more particularly, to the epitope, and an antibody or an antigen-binding fragment binding thereto. The epitope region of the human Trx1 antigen confirmed in the present invention may be effectively used in the development of an improved antibody to enhance the binding affinity of an anti-Trx1 antibody. In addition, the improved antibody of the present invention is effective in improvement of performance of a breast cancer diagnosis kit due to excellent binding affinity for Trx1 and very high sensitivity and specificity, compared to a conventional anti-Trx1 antibody. Further, the accuracy and reliability of breast cancer diagnosis may significantly increase because exceptionally high sensitivity and specificity are exhibited by detecting the monoclonal antibody of the present invention, which specifically binds to Trx1, rather than detecting CA15-3, another conventional breast cancer diagnostic biomarker.

公开(公告)号：US11938250B2

公开(公告)日：2024-03-26

申请号：US16272808

申请日：2019-02-11

Applicant: Surmodics, Inc.

Inventor： David E. Babcock ,  Sean Lundquist ,  Gary Opperman ,  Adriyn Torguson ,  Tim Jentz

IPC: A61L29/08 ,  A61K31/7024 ,  A61L15/44 ,  A61L27/34 ,  A61L27/54 ,  A61L29/06 ,  A61L29/16 ,  A61L31/10 ,  A61L31/16 ,  G01N33/543

CPC classification number: A61L29/085 ,  A61K31/7024 ,  A61L15/44 ,  A61L27/34 ,  A61L27/54 ,  A61L29/06 ,  A61L29/16 ,  A61L31/10 ,  A61L31/16 ,  G01N33/54393 ,  A61L2300/216 ,  A61L2300/418 ,  A61L2300/42 ,  A61L2400/04 ,  A61L2400/18 ,  A61L2420/02 ,  A61L2420/08

Abstract: Embodiments of the disclosure include coatings comprising an oligomerized polyphenol layer. The oligomerized polyphenol layer can be used as an intermediate coated layer on a medical device that hydrogen bonds to a synthetic or natural polymer, which in turn can be used as a top coat or further associated with another coated layer. The multilayered coatings can provide properties such as hemocompatibility or lubricity. In other embodiments, the oligomerized polyphenol layer is used on a medical device as a hemostatic layer configured to contact blood and promote coagulation. The oligomerized polyphenol layer can also be used on the inner surface (e.g., inner diameter) of a medical device to prevent bacterial adherence. The oligomerized polyphenol layer can also be used on the surface of a in vitro diagnostic article, or a cell culture device to, promote adsorption of a biological molecule.

公开(公告)号：US20240067805A1

公开(公告)日：2024-02-29

申请号：US18492980

申请日：2023-10-24

Applicant: NB Postech

Inventor： Joon Won PARK ,  Sung Min SEO

IPC: C08L1/18 ,  B01D67/00 ,  B01D71/20 ,  G01N33/543

CPC classification number: C08L1/18 ,  B01D67/0088 ,  B01D71/20 ,  G01N33/54393

Abstract: The present invention provides an improved method of quantitative and/or qualitative analysis of a target molecule using nitrocellulose membrane (NCM). In particular, the present invention provides a porous nitrocellulose membrane that includes a surface and an organic nanostructured molecule that is non-covalently attached to the surface of NCM. The organic nanostructured molecule has a branched region that includes a plurality of terminal region (e.g., terminal end) moieties that are non-covalently attached or bound to a surface of the porous NCM. The organic nanostructured molecule also comprises a linear region that includes a covalently attached capture molecule that is adapted to selectively bind to a target molecule. The NCM of the invention provides an improved reproducibility, reliability, and selectivity compared an NCM in the absence of the organic nanostructured molecule.

公开(公告)号：US20240053335A1

公开(公告)日：2024-02-15

申请号：US18268932

申请日：2021-12-20

Applicant: The Board of Trustees of the Leland Stanford Junior University ,  Microdrop Inc.

Inventor： Gary Peltz ,  Feng Hu

IPC: G01N33/543 ,  G01N33/94 ,  C12Q1/6806 ,  C12Q1/6858

CPC classification number: G01N33/54393 ,  G01N33/948 ,  G01N33/9486 ,  G01N33/946 ,  C12Q1/6806 ,  C12Q1/6858

Abstract: Provided are methods of analyzing a capillary microsample obtained from a subject. In certain embodiments, the methods comprise assessing a test mixture for one or more capillary microsample analytes, wherein the test mixture comprises the capillary microsample diluted into a stabilizing buffer comprising isopropanol, ethanol, methanol, or a combination thereof. Such methods further comprise analyzing capillary microsample nucleic acids purified from the test mixture. According to some embodiments, analyzing the capillary microsample nucleic acids comprises genotyping the subject. Methods of determining one or more alleles of a subject are also provided, as are kits that find use in practicing the methods of the present disclosure.