公开(公告)号：US20240301468A1

公开(公告)日：2024-09-12

申请号：US18652896

申请日：2024-05-02

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Je-hyuk Lee ,  Richard C. Terry ,  Evan R. Daugharthy

IPC: C12Q1/6806 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874

CPC classification number: C12Q1/6806 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

公开(公告)号：US11447807B2

公开(公告)日：2022-09-20

申请号：US16288200

申请日：2019-02-28

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Evan R. Daugharthy ,  Richard C. Terry

IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/682 ,  C12Q1/6834 ,  C12Q1/6874 ,  G01N33/68 ,  A61L27/50

Abstract: The present disclosure provides methods that combine RNA fluorescent in situ sequencing (FISSEQ) with other molecular detection modalities, forming an integrated panomic detection platform. In various embodiments, the present disclosure provides systems and methods to prepare a biological sample to preserve the spatial relationships of biomolecules of interest within the biological sample for FISSEQ detection.

公开(公告)号：US11111521B2

公开(公告)日：2021-09-07

申请号：US17122168

申请日：2020-12-15

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Je-hyuk Lee ,  Richard C. Terry ,  Evan R. Daugharthy

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6844

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

公开(公告)号：US20220228196A1

公开(公告)日：2022-07-21

申请号：US17708050

申请日：2022-03-30

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Je-Hyuk Lee ,  Richard C. Terry ,  Evan R. Daugharthy

IPC: C12Q1/6806 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

公开(公告)号：US20210381049A1

公开(公告)日：2021-12-09

申请号：US17392325

申请日：2021-08-03

Applicant: President and Fellows of Harvard College

Inventor： Evan R. Daugharthy ,  George M. Church

IPC: C12Q1/6876 ,  C12Q1/682 ,  C12N15/115 ,  C12Q1/6806 ,  C12Q1/6816

Abstract: The disclosure provides a method for detecting a target analyte in a biological sample including contacting the sample with one or more probe sets each comprising a primary probe and a linker, contacting the sample with an initiator sequence, contacting the sample with a plurality of fluorescent DNA hairpins, wherein the probe binds the target molecule, the linker connects the probe to the initiator sequence, and wherein the initiator sequence nucleates with the cognate hairpin and triggers self-assembly of tethered fluorescent amplification polymers, and detecting the target molecule by measuring fluorescent signal of the sample.

公开(公告)号：US11085072B2

公开(公告)日：2021-08-10

申请号：US16285292

申请日：2019-02-26

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Evan R. Daugharthy ,  Richard C. Terry ,  Benjamin W. Pruitt ,  Brian M. Turczyk

IPC: C12Q1/6832 ,  C12Q1/6806 ,  C12N15/10 ,  C12Q1/6816 ,  C12Q1/6874 ,  C12Q1/6844 ,  C12Q1/6869

Abstract: The present disclosure provides a number of targeted nucleic acid FISSEQ library construction methods. Targeted FISSEQ can exhibit several benefits, such as enhanced sensitivity and/or shorter assay time in the detection, identification, quantification, and/or determining the nucleotide sequence of the target species, relative to “random” or “whole-omic” detection via FISSEQ.

公开(公告)号：US20190241950A1

公开(公告)日：2019-08-08

申请号：US16386337

申请日：2019-04-17

Applicant: President and Fellows of Harvard College

Inventor： Evan R. Daugharthy ,  Richard C. Terry ,  Je-hyuk Lee ,  George M. Church ,  Benjamin W. Pruitt

IPC: C12Q1/6869 ,  C12Q1/6841 ,  C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/68 ,  C12Q1/6841 ,  C12Q2543/101 ,  C12Q2563/159 ,  C12Q2565/601

Abstract: Methods of volumetric imaging of a three-dimensional matrix of nucleic acids within a cell is provided. An automated apparatus for sequencing and volumetric imaging of a three-dimensional matrix of nucleic acids is provided.

公开(公告)号：US20190155835A1

公开(公告)日：2019-05-23

申请号：US15772652

申请日：2016-11-03

Applicant: President and Fellows of Harvard College

Inventor： Evan R. Daugharthy ,  Vivek Dasari ,  George M. Church

IPC: G06F16/532 ,  G06T7/90 ,  G06T7/70 ,  G06F16/538 ,  G16B30/00

Abstract: Methods, systems, and computer-readable media for processing spatially related sequence data received from a sequencing device are presented. In one or more embodiments, a computing platform may receive, from a sequencing device, image data associated with a sample. The computing platform may identify, based on the image data received from the sequencing device, a first sequence located at first spatial coordinates. Subsequently, the computing platform may store, in a spatially searchable database, a first data element comprising the first spatial coordinates and a first identifier corresponding to the first sequence to spatially relate the first sequence to other sequences present in the sample. In some instances, the image data received from the sequencing device may include spatial information, temporal information, and color information associated with the sample, and the computing platform may present, on a display device, information identifying a presence of the first sequence at the first spatial coordinates.

公开(公告)号：US20190085383A1

公开(公告)日：2019-03-21

申请号：US16200831

申请日：2018-11-27

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Je-Hyuk Lee ,  Evan R. Daugharthy

IPC: C12Q1/6841 ,  C07H21/02 ,  C07H21/04

Abstract: Methods of labelling one or more subcellular components (e.g., an organelle and/or subcellular region) in vivo are provided. Methods of labelling a protein in vivo are provided. Methods of determining a nucleic acid sequence in situ are also provided.

公开(公告)号：US20210324450A1

公开(公告)日：2021-10-21

申请号：US17363097

申请日：2021-06-30

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Je-Hyuk Lee ,  Richard C. Terry ,  Evan R. Daugharthy

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6869

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.