-
公开(公告)号：US11993773B2
公开(公告)日：2024-05-28
申请号：US17192578
申请日：2021-03-04
申请人： DNA Script SAS
发明人： Xavier Godron , Adrian Horgan , Sylvain Gariel , Jeffrey Jeddeloh , Robert Nicol , Thomas Ybert
CPC分类号： C12N15/1096 , C12N15/111 , C12Q1/44 , C40B50/06 , C40B50/08 , C40B50/14 , C40B70/00 , C12N2310/319 , C12N2310/3515 , C12N2330/31
摘要： The invention is directed to methods for synthesizing oligonucleotides directly on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3′-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3′ hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.
-
公开(公告)号：US20220186213A1
公开(公告)日：2022-06-16
申请号：US17687128
申请日：2022-03-04
申请人： DNA Script SAS
发明人： Xavier Godron , Adrian Horgan , Sylvain Gariel , Jeffrey Jeddeloh , Robert Nicol , Thomas Ybert
摘要： The invention is directed to methods for synthesizing oligonucleotides direction on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3′-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3′ hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.
-
公开(公告)号：US20220403435A1
公开(公告)日：2022-12-22
申请号：US17629322
申请日：2020-07-28
申请人： DNA Script SAS
发明人： Adrian Horgan , Ivo Sarac , Supaporn Niyomchon , Xavier Godron
摘要： The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides using chain elongation conditions that suppress the formation of DNA secondary structures including, but not limited to, intra-strand and between-strand duplexes, G-quadruplexes, and the like. In some embodiments, such chain elongation conditions include using 3′-O-blocked dNTP monomers that base protection groups or base analogs that suppress the formation of hydrogen bonding in the polynucleotide being synthesized.
-
公开(公告)号：US11268091B2
公开(公告)日：2022-03-08
申请号：US16970590
申请日：2019-12-10
申请人： DNA Script SAS
发明人： Xavier Godron , Adrian Horgan , Sylvain Gariel , Jeffrey Jeddeloh , Robert Nicol , Thomas Ybert
摘要： The invention is directed to methods for synthesizing oligonucleotides directly on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3′-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3′ hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.
-
-
-
搜索结果
4 条记录 (耗时 0.013 秒)
