公开(公告)号：US12006540B2

公开(公告)日：2024-06-11

申请号：US17816555

申请日：2022-08-01

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Xiaoxu Li ,  Xin Chen ,  Zengmin Li ,  Shiv Kumar ,  Shundi Shi ,  Cheng Guo ,  Jianyi Ren ,  Min-Kang Hsieh ,  Minchen Chien ,  Chuanjuan Tao ,  Ece Erturk ,  Sergey Kalachikov ,  James J. Russo

IPC分类号： C12Q1/68 ,  C07H19/10 ,  C07H19/14 ,  C07H19/20 ,  C12Q1/6869

CPC分类号： C12Q1/6869 ,  C07H19/10 ,  C07H19/14 ,  C07H19/20 ,  C12Q1/6869 ,  C12Q2525/117 ,  C12Q2563/107

摘要： Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing of a nucleic acid.

公开(公告)号：US20220364167A1

公开(公告)日：2022-11-17

申请号：US17860036

申请日：2022-07-07

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Jingyue Ju ,  Shiv Kumar ,  James J. Russo ,  Steffen Jockusch ,  Zengmin Li ,  Xiaoxu Li ,  Sergey M. Kalachikov ,  Irina Morozova

IPC分类号： C12Q1/6869 ,  C07H19/10 ,  C07H19/20

摘要： This invention provides nucleotide analogues each of which comprises a tag comprising one or more Forster resonance energy transfer (FRET) acceptor fluorophores, a nucleotide polymerase having one or more FRET donor fluorophores, and methods for sequencing single-stranded

公开(公告)号：US20200069717A1

公开(公告)日：2020-03-05

申请号：US16303715

申请日：2017-05-23

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Jingyue Ju ,  Xin Chen ,  Xiaoxu Li ,  Zengmin Li ,  Min-Kang Hsieh ,  Minchen Chien ,  Shundi Shi ,  Jianyi Ren ,  Cheng Guo ,  Shiv Kumar ,  James J. Russo ,  Chuanjuan Tao ,  Steffen Jockusch ,  Sergey Kalachikov

IPC分类号： A61K31/7052 ,  C07H21/04

摘要： Disclosed herein, inter alia, are compounds, compositions, and methods of thereof in the sequencing a nucleic acid.

公开(公告)号：US20190153527A1

公开(公告)日：2019-05-23

申请号：US16091442

申请日：2017-04-04

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Jingyue Ju ,  Shiv Kumar ,  James J. Russo ,  Steffen Jockusch ,  Zengmin Li ,  Xiaoxu Li ,  Sergey M. Kalachikov ,  Irina Morozova

IPC分类号： C12Q1/6869 ,  C07H19/10 ,  C07H19/20

摘要： This invention provides nucleotide analogues each of which comprises a tag comprising one or more Forster resonance energy transfer (FRET) acceptor fluorophores, a nucleotide polymerase having one or more FRET donor fluorophores, and methods for sequencing single-stranded

公开(公告)号：US20190085014A1

公开(公告)日：2019-03-21

申请号：US16149114

申请日：2018-10-01

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C07H19/14 ,  C12Q1/6869 ,  C07H21/00 ,  C12Q1/686 ,  C12Q1/6872 ,  C12Q1/6876 ,  C07H19/10 ,  C12Q1/68 ,  C12Q1/6874 ,  C40B40/00

CPC分类号： C12Q1/6869 ,  C07B2200/11 ,  C07H19/10 ,  C07H19/14 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2525/186 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C12Q2565/501 ,  C40B40/00

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US20190031705A1

公开(公告)日：2019-01-31

申请号：US16150185

申请日：2018-10-02

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C07H19/14 ,  C12Q1/6869 ,  C07H19/10 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6872 ,  C12Q1/68 ,  C12Q1/686

CPC分类号： C12Q1/6869 ,  C07B2200/11 ,  C07H19/10 ,  C07H19/14 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2525/186 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C12Q2565/501 ,  C40B40/00

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US09725480B2

公开(公告)日：2017-08-08

申请号：US15167917

申请日：2016-05-27

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C07H19/14 ,  C12Q1/68 ,  C07H21/00 ,  C40B40/00

CPC分类号： C07H19/14 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C40B40/00 ,  C12Q2565/501 ,  C12Q2525/186

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US20230160000A1

公开(公告)日：2023-05-25

申请号：US17818705

申请日：2022-08-09

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Xiaoxu Li ,  Xin Chen ,  Zengmin Li ,  Shiv Kumar ,  Shundi Shi ,  Cheng Guo ,  Jianyi Ren ,  Min-Kang Hsieh ,  Minchen Chien ,  Chuanjuan Tao ,  Ece Erturk ,  Sergey Kalachikov ,  James J. Russo

IPC分类号： C12Q1/6869 ,  C07H19/10 ,  C07H19/14 ,  C07H19/20

CPC分类号： C12Q1/6869 ,  C07H19/10 ,  C07H19/14 ,  C07H19/20

摘要： Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing of a nucleic acid.

公开(公告)号：US20220290227A1

公开(公告)日：2022-09-15

申请号：US17576184

申请日：2022-01-14

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Dae Hyun Kim ,  Jia Guo ,  Qinglin Meng ,  Zengmin Li ,  Huanyan Cao

IPC分类号： C12Q1/6874 ,  C07H19/04 ,  C12Q1/6869 ,  C12Q1/6876

摘要： This invention provides a process for sequencing nucleic acids using 3′ modified deoxynucleotide analogues or 3′ modified deoxyinosine triphosphate analogues, and 3′ modified dideoxynucleotide analogues having a detectable marker attached thereto.

公开(公告)号：US11266673B2

公开(公告)日：2022-03-08

申请号：US16303715

申请日：2017-05-23

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Jingyue Ju ,  Xin Chen ,  Xiaoxu Li ,  Zengmin Li ,  Min-Kang Hsieh ,  Minchen Chien ,  Shundi Shi ,  Jianyi Ren ,  Cheng Guo ,  Shiv Kumar ,  James J. Russo ,  Chuanjuan Tao ,  Steffen Jockusch ,  Sergey Kalachikov

IPC分类号： C12Q1/68 ,  A61K31/7052 ,  C07H21/04 ,  C07H19/20 ,  C12Q1/6869

摘要： Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing a nucleic acid.