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    TAMPER-RESISTANT ASSEMBLY FOR SECURING VALUABLE MATERIAL
    2.
    发明申请
    TAMPER-RESISTANT ASSEMBLY FOR SECURING VALUABLE MATERIAL 有权
    用于保护有价值材料的防篡改组件

    公开(公告)号：US20150314927A1

    公开(公告)日：2015-11-05

    申请号：US14702378

    申请日：2015-05-01

    Applicant: Synthetic Genomics, Inc.

    Inventor： Laurence Warden ,  Tei Newman-Lehman ,  Daniel G. Gibson

    IPC: B65D43/02 ,  B65B7/28 ,  G01N21/64 ,  B65D25/20 ,  B65D25/10 ,  B65B5/04 ,  B65C1/04

    CPC classification number: B65D61/00 ,  B01L3/50851 ,  B01L9/523 ,  B01L2200/026 ,  B01L2200/028 ,  B01L2200/0689 ,  B01L2200/141 ,  B01L2300/0829 ,  B01L2300/0887 ,  B65B5/04 ,  B65B7/28 ,  B65C1/04 ,  B65D25/108 ,  B65D25/205 ,  B65D43/0235 ,  B65D2101/0007 ,  B65D2101/0092 ,  G01N21/6428 ,  G01N21/6486 ,  G01N21/77 ,  G01N2021/6439

    Abstract: The invention provides a tamper resistant assembly that that securely contains a valuable material. The assembly has a container for holding the valuable material, an optional carrier that contains the container, one or more cover components that enclose the valuable material in the container, and one or more labels having a plurality of devices that reveal tampering by distortion of at least one of the plurality of devices. The label(s) are positioned so that dislodging a cover component causes a detectable distortion in at least one of the plurality of devices, thereby revealing tampering with the assembly. In one embodiment the label can be affixed partially to a surface of a cover component and partially to a surface of the container or the optional carrier. Also disclosed are methods of detecting tampering and method of manufacturing a temper-resistant assembly. Because the assembly allows a remote validator to validate the assembly prior to providing essential instructions or authorization for conducting procedures on valuable material contained by the assembly, the manufacturer is assured that its procedures are being provided only to authorized persons.

    Abstract translation: 本发明提供一种安全地包含有价值材料的防篡改组件。 该组件具有用于容纳有价值的材料的容器，容纳容器的可选载体，将容器中的有价值材料包围的一个或多个覆盖部件，以及一个或多个标签，其具有多个通过在 多个装置中的至少一个。 定位标签使得移除覆盖部件会在多个装置中的至少一个装置中引起可检测的变形，由此显示对装置的篡改。 在一个实施例中，标签可以部分地固定到盖部件的表面并且部分地固定到容器或可选的载体的表面。 还公开了检测篡改的方法和制造耐回火组件的方法。 因为组装允许远程验证器在组装之前提供必要的指令或授权进行有价值的材料的程序的验证，所以制造商保证其程序仅被提供给授权的人员。

    METHOD OF NUCLEIC ACID CASSETTE ASSEMBLY
    3.
    发明申请
    METHOD OF NUCLEIC ACID CASSETTE ASSEMBLY 审中-公开

    公开(公告)号：US20180340165A1

    公开(公告)日：2018-11-29

    申请号：US16056343

    申请日：2018-08-06

    Applicant: Synthetic Genomics, Inc.

    Inventor： J. Craig Venter ,  Hamilton O. Smith ,  Clyde A. Hutchison, III ,  Daniel G. Gibson

    IPC: C12N15/10 ,  C12N15/66

    Abstract: Methods are provided for constructing a synthetic genome, comprising generating and assembling nucleic acid cassettes comprising portions of the genome, wherein at least one of the nucleic acid cassettes is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. Synthetic genomes or synthetic cells may be used for a variety of purposes, including the generation of synthetic fuels, such as hydrogen or ethanol.

    PEG-MEDIATED ASSEMBLY OF NUCLEIC ACID MOLECULES
    5.
    发明申请
    PEG-MEDIATED ASSEMBLY OF NUCLEIC ACID MOLECULES 审中-公开

    公开(公告)号：US20190177759A1

    公开(公告)日：2019-06-13

    申请号：US16212534

    申请日：2018-12-06

    Applicant: SYNTHETIC GENOMICS, INC.

    Inventor： ZHIQING Qi ,  Jun Urano ,  Nicky C. Caiazza ,  Daniel G. Gibson

    IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/6844

    Abstract: The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a DNA polymerase, a mixture of dNTPs, and a crowding agent to form an assembly mixture. In one embodiment the crowding agent is polyethylene glycol (PEG). The presence of the crowding agent facilitates the nucleic acid assembly process of the invention. The assembly mixture is then subjected to multiple cycles, each cycle comprising an annealing phase, an extension phase, and a denaturation phase, and the desired nucleic acid molecule is thereby assembled. In some embodiments one or more of the phases are time varied.

    Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
    6.
    发明授权
    Methods for in vitro joining and combinatorial assembly of nucleic acid molecules 有权

    公开(公告)号：US10266865B2

    公开(公告)日：2019-04-23

    申请号：US14636082

    申请日：2015-03-02

    Applicant: Synthetic Genomics, Inc.

    Inventor： Daniel G. Gibson ,  Hamilton O. Smith ,  Clyde A. Hutchison ,  Lei Young ,  J. Craig Venter

    IPC: C12P19/34 ,  C12N15/10 ,  C12N15/64 ,  C12N15/66

    Abstract: The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization.

    METHODS FOR ASSEMBLING DNA MOLECULES
    7.
    发明申请
    METHODS FOR ASSEMBLING DNA MOLECULES 审中-公开

    公开(公告)号：US20180163254A1

    公开(公告)日：2018-06-14

    申请号：US15839597

    申请日：2017-12-12

    Applicant: SYNTHETIC GENOMICS, INC.

    Inventor： JOHN E. GILL ,  Daniel G. Gibson ,  Lixia Fu

    IPC: C12Q1/686 ,  C07H19/173 ,  C07H19/073 ,  C12N9/22

    CPC classification number: C12Q1/686 ,  C07H19/073 ,  C07H19/173 ,  C12N9/22 ,  C12N15/09 ,  C12N15/10 ,  C12N15/1031 ,  C12P19/34 ,  C12Y302/02027 ,  C12Q2521/101 ,  C12Q2533/101

    Abstract: The invention provides compositions and methods for assembling a DNA molecule having a desired sequence. The methods involve contacting a DNA polymerase, dNTPs, and a plurality of pairs of oligonucleotides. The oligonucleotides of a pair have a portion of the desired sequence, and an internal sequence that overlaps and is complementary to an internal sequence of the other oligonucleotide of the pair, and, when arranged in order, they have at least a portion of the desired sequence. The oligonucleotides also have a 3′ or a 5′ primer binding sequence having a binding site for a primer. The oligonucleotides that correspond to the end oligonucleotides of the desired sequence also have a universal 3′ flanking sequence and a universal 5′ flanking sequence, respectively. The methods involve performing a first amplification reaction on the plurality of pairs of oligonucleotides; removing the 3′ and 5′ primer binding sequences from the plurality of pairs of oligonucleotides; and subjecting the plurality of pairs of oligonucleotides to an assembly reaction to thereby assemble the dsDNA molecule having the desired sequence.

    PEG-MEDIATED ASSEMBLY OF NUCLEIC ACID MOLECULES
    9.
    发明申请
    PEG-MEDIATED ASSEMBLY OF NUCLEIC ACID MOLECULES 审中-公开
    PEG介导的核酸分子组装

    公开(公告)号：US20140308710A1

    公开(公告)日：2014-10-16

    申请号：US14103578

    申请日：2013-12-11

    Applicant: Synthetic Genomics, Inc.

    Inventor： Zhiqing Qi ,  Jun Urano ,  Nicky C. Caiazza ,  Daniel G. Gibson

    IPC: C12P19/34

    CPC classification number: C12P19/34 ,  C12N15/1027 ,  C12N15/1031 ,  C12Q1/6844 ,  C12Q2521/101 ,  C12Q2527/101 ,  C12Q2527/125 ,  C12Q2527/143 ,  C12Q2533/107

    Abstract: The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a DNA polymerase, a mixture of dNTPs, and a crowding agent to form an assembly mixture. In one embodiment the crowding agent is polyethylene glycol (PEG). The presence of the crowding agent facilitates the nucleic acid assembly process of the invention. The assembly mixture is then subjected to multiple cycles, each cycle comprising an annealing phase, an extension phase, and a denaturation phase, and the desired nucleic acid molecule is thereby assembled. In some embodiments one or more of the phases are time varied.

    Abstract translation: 本发明公开了从一组重叠寡核苷酸组装核酸分子的方法。 该方法包括将一组重叠的寡核苷酸与DNA聚合酶，dNTP的混合物和拥挤剂接触以形成组合混合物。 在一个实施方案中，拥挤剂是聚乙二醇（PEG）。 拥挤剂的存在促进了本发明的核酸组装过程。 然后将组合混合物进行多个循环，每个循环包含退火阶段，延伸相和变性阶段，从而组装所需的核酸分子。 在一些实施方案中，一个或多个相是时间变化的。

    VIBRIO SP. ORGANISMS WITH MODIFIED LIPOPOLYSACCHARIDE
    10.
    发明申请
    VIBRIO SP. ORGANISMS WITH MODIFIED LIPOPOLYSACCHARIDE 审中-公开

    公开(公告)号：US20190153554A1

    公开(公告)日：2019-05-23

    申请号：US16154618

    申请日：2018-10-08

    Applicant: SYNTHETIC GENOMICS, INC.

    Inventor： Matthew T Weinstock ,  Daniel G. Gibson ,  Daniel Strimling

    IPC: C12R1/63 ,  C12N15/74

    Abstract: The invention provides engineered Vibrio sp. organisms that comprise a genetic modification to either or both of the lpxL and/or lpxM genes. The organisms score substantially lower in an in vitro endotoxin assay versus the unmodified or wild type organism. The organisms preserve substantially the growth rate of the corresponding unmodified organisms. The organisms can also have an exogenous nucleic acid cloned in the organism, or an exogenous nucleic acid encoding a protein, polypeptide, or peptide expressed by the organism, and optionally secreted from the organism.

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