公开(公告)号：US20160177322A1

公开(公告)日：2016-06-23

申请号：US15051463

申请日：2016-02-23

Applicant: Synthetic Genomics, Inc.

Inventor： Gwynedd A. Benders ,  John I. Glass ,  Clyde A. Hutchison, III ,  Carole Lartigue ,  Sanjay Vashee ,  Mikkel A. Algire ,  Hamilton O. Smith ,  Charles E. Merryman ,  Vladimir N. Noskov ,  Ray-Yuan Chuang ,  Daniel G. Gibson ,  J. Craig Venter

IPC: C12N15/81 ,  C12N15/10

CPC classification number: C12N15/81 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1031 ,  C12N15/1079 ,  C12N15/1093

Abstract: Compositions and methods are disclosed herein for cloning a synthetic or a semi-synthetic donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.

Abstract translation: 本文公开了在异源宿主细胞中克隆合成或半合成供体基因组的组合物和方法。 在一个实施方案中，可以在宿主细胞内进一步修饰供体基因组。 修饰或未修饰的基因组可以进一步从宿主细胞中分离并转移到受体细胞。 本文公开的方法可用于在更容易处理的宿主细胞中改变来自难治性供体细胞的供体基因组。

公开(公告)号：US20180119132A1

公开(公告)日：2018-05-03

申请号：US15466675

申请日：2017-03-22

Applicant: Synthetic Genomics, Inc.

Inventor： Clyde A. Hutchison, III ,  Ray-Yuan Chuang ,  Vladimir N. Noskov ,  Bogumil J. Karas ,  Kim S. Wise ,  Hamilton O. Smith ,  John I. Glass ,  Chuck Merryman ,  Daniel G. Gibson ,  J. Craig Venter ,  Krishna Kannan ,  Lin Ding

IPC: C12N15/10 ,  C12N15/74 ,  C12N15/81 ,  G06F19/22

CPC classification number: C12N15/1027 ,  C12N15/1058 ,  C12N15/1079 ,  C12N15/1093 ,  C12N15/74 ,  C12N15/81 ,  G16B30/00

Abstract: Methods for generating synthetic genomes, for example synthetic genomes having desired properties or viable genomes of reduced size, are disclosed. Also disclosed are synthetic genomes produced by the methods disclosed herein and synthetic cells containing the synthetic genomes disclosed herein.

公开(公告)号：US20160177338A1

公开(公告)日：2016-06-23

申请号：US15056796

申请日：2016-02-29

Applicant: Synthetic Genomics, Inc.

Inventor： Gwynedd A. Benders ,  John I. Glass ,  Clyde A. Hutchison, III ,  Carole Lartigue ,  Sanjay Vashee ,  Mikkel A. Algire ,  Hamilton O. Smith ,  Charles E. Merryman ,  Vladimir N. Noskov ,  Ray-Yuan Chuang ,  Daniel G. Gibson ,  J. Craig Venter

IPC: C12N15/90 ,  C12N15/81

CPC classification number: C12N15/905 ,  C12N15/10 ,  C12N15/1031 ,  C12N15/1079 ,  C12N15/1093 ,  C12N15/81

Abstract: Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.

Abstract translation: 本文公开了用于在异源宿主细胞中克隆供体基因组的组合物和方法。 在一个实施方案中，可以在宿主细胞内进一步修饰供体基因组。 修饰或未修饰的基因组可以进一步从宿主细胞中分离并转移到受体细胞。 本文公开的方法可以用于在更容易处理的宿主细胞中改变来自难治性供体细胞的供体基因组。