公开(公告)号：US12110498B2

公开(公告)日：2024-10-08

申请号：US17690429

申请日：2022-03-09

申请人： AMYRIS, INC.

发明人： Kevin George ,  Andrew Main ,  Chia-Hong Tsai

IPC分类号： C12N15/70 ,  C12N1/19 ,  C12N15/10 ,  C12N15/81 ,  C12N15/90

CPC分类号： C12N15/905 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/81 ,  C12N15/1082 ,  C12Q2563/179

摘要： Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.

公开(公告)号：US20240263199A1

公开(公告)日：2024-08-08

申请号：US18604597

申请日：2024-03-14

申请人： Jiangnan University

发明人： Xueqin Lv ,  Long Liu ,  Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Yanfeng Liu ,  Tiantian Liu

IPC分类号： C12P7/56 ,  C12N1/18 ,  C12N9/04 ,  C12N15/90 ,  C12R1/865

CPC分类号： C12P7/56 ,  C12N1/185 ,  C12N9/0006 ,  C12N15/905 ,  C12Y101/01027 ,  C12Y101/05005 ,  C12R2001/865

摘要： The present disclosure discloses a method for improving production of L-lactic acid (L-LA) by Saccharomyces cerevisiae based on regulation and control of ethanol metabolic flux, and belongs to the technical field of microorganisms. According to the present disclosure, acid-resistant Saccharomyces cerevisiae TJG16 is used as a production strain, an ethanol dehydrogenase gene adhA derived from Bacillus subtilis is introduced to promote conversion of ethanol into acetaldehyde, and a lactate aldolase gene BAL derived from Brucella sp. is introduced to promote synthesis of lactic acid from the acetaldehyde. Moreover, an acetaldehyde dehydrogenase gene ALD6 is knocked out to prevent synthesis of acetic acid from the acetaldehyde, a transcriptional regulatory factor encoding gene GAL80 for regulating and controlling galactose is knocked out, and lactate dehydrogenase LDH is integrated, so that the L-LA is finally increased.

公开(公告)号：US20240182927A1

公开(公告)日：2024-06-06

申请号：US18199278

申请日：2023-05-18

申请人： Amyris, Inc.

发明人： Jessica WALTER

IPC分类号： C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N15/81

CPC分类号： C12N15/905 ,  C12N9/22 ,  C12N15/11 ,  C12N15/815 ,  C12N2310/20 ,  C12N2800/80

摘要： The present invention provides high efficiency targeted and marker-less single or simultaneous multiple integrations using nucleases and a stable plasmid in Kluyveromyces host cells.

公开(公告)号：US20240043855A1

公开(公告)日：2024-02-08

申请号：US18258799

申请日：2021-12-12

申请人： TOYOTA JIDOSHA KABUSHIKI KAISHA

发明人： Toru ONISHI

IPC分类号： C12N15/81 ,  C12N15/90

CPC分类号： C12N15/81 ,  C12N15/905

摘要： This invention is intended to simply and efficiently produce a stable transformant comprising a gene of interest incorporated into the genome. By introducing linear nucleic acid fragments for gemone-introduction comprising a gene of interest and a helper plasmid for transformation comprising a pair of homologous recombination sequences to incorporate the fragment and functioning a counter selection marker, a host comprising the helper plasmid for transformation into which the linear nucleic acid fragment is incorporated. Results in death.

公开(公告)号：US20230407340A1

公开(公告)日：2023-12-21

申请号：US18178275

申请日：2023-03-03

申请人： B0LT THREADS, INC.

发明人： Thomas Stevens

IPC分类号： C12N15/90 ,  C07K16/14 ,  C12N15/62

CPC分类号： C12N15/905 ,  C07K16/14 ,  C12N15/625

摘要： Disclosed herein are modified strains for improving secretion of recombinantly expressed products secreted from a host organism with improved growth and productivity characteristics, as well as methods of using the modified strains.

公开(公告)号：US20230364268A1

公开(公告)日：2023-11-16

申请号：US18356029

申请日：2023-07-20

申请人： SNIPR Technologies Limited

发明人： Jasper CLUBE

IPC分类号： A61K48/00 ,  C12N15/90 ,  C12N15/63 ,  C12N9/22 ,  A61P31/04 ,  A61K35/74 ,  C12P19/34

CPC分类号： A61K48/0066 ,  C12N15/907 ,  C12N15/63 ,  C12N9/22 ,  A61P31/04 ,  A61K35/74 ,  C12N15/905 ,  C12P19/34 ,  C12N2800/80 ,  C12N15/902 ,  C12N2310/20 ,  C12N15/70

摘要： The invention relates to retrieving or modifying target nucleic acids, such as host cell chromosomal DNA, by homologous recombination with vectors that have been cut to provide recombinogenic nucleic acid strands in situ.

公开(公告)号：US11685934B2

公开(公告)日：2023-06-27

申请号：US16646028

申请日：2018-09-12

申请人： Amyris, Inc.

发明人： Jessica Walter

IPC分类号： C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N15/81

CPC分类号： C12N15/905 ,  C12N9/22 ,  C12N15/11 ,  C12N15/815 ,  C12N2310/20 ,  C12N2800/80

摘要： The present invention provides high efficiency targeted and marker-less single or simultaneous multiple integrations using nucleases and a stable plasmid in Kluyveromyces host cells.

公开(公告)号：US20230183686A1

公开(公告)日：2023-06-15

申请号：US17933005

申请日：2022-09-16

申请人： BASF Plant Science Company GmbH

发明人： Leendert W. Neuteboom ,  John A. McElver ,  Rosa De Pinho Barroco ,  Chris De Wilde ,  Max Fabian Felle

IPC分类号： C12N15/11 ,  C12N15/10 ,  C12N15/70 ,  C12N15/75 ,  C12N15/82 ,  C12N15/85 ,  C12N15/90 ,  C12N15/81

CPC分类号： C12N15/11 ,  C12N15/102 ,  C12N15/111 ,  C12N15/70 ,  C12N15/75 ,  C12N15/8274 ,  C12N15/8509 ,  C12N15/902 ,  C12N15/81 ,  C12N2310/20 ,  C12N15/905 ,  C12N15/907

摘要： Methods for modification of target nucleic acids. The method involves a construct in which guide RNA is covalently linked to donor RNA (fusion NA) to be introduced into the target nucleic acid by homologous recombination and is based on the introduction of a nuclease, e.g. CRISPR or TALEN, into the cell containing the target nucleic acid. The fusion NA may be introduced as a DNA vector.

公开(公告)号：US20180320164A1

公开(公告)日：2018-11-08

申请号：US15970728

申请日：2018-05-03

申请人： California Institute of Technology

发明人： Bruce A Hay ,  Georg Oberhofer ,  Tobin William Ivy

IPC分类号： C12N15/10 ,  C12N15/90 ,  C12N5/14 ,  C12N5/16

CPC分类号： C12N15/102 ,  C12N5/14 ,  C12N5/16 ,  C12N15/74 ,  C12N15/80 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8283 ,  C12N15/8286 ,  C12N15/905 ,  C12N15/907 ,  C12N2310/20

摘要： Described herein are embodiments relating to manipulation of populations and sex ratio in populations through DNA sequence modifications.

公开(公告)号：US09982279B1

公开(公告)日：2018-05-29

申请号：US15632001

申请日：2017-06-23

申请人： Inscripta, Inc.

发明人： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb

IPC分类号： C12N15/90 ,  C12N15/11 ,  C12N15/10 ,  C12N15/85 ,  C12N9/22 ,  C12N5/10 ,  C12N5/14 ,  C12N5/16 ,  C12N5/22 ,  C12N15/09 ,  C12N15/52 ,  C12N15/63 ,  C12N15/79 ,  C12N15/70 ,  C12N15/81

CPC分类号： C12N15/902 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/905 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/22

摘要： Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.