公开(公告)号：US20240263199A1

公开(公告)日：2024-08-08

申请号：US18604597

申请日：2024-03-14

申请人： Jiangnan University

发明人： Xueqin Lv ,  Long Liu ,  Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Yanfeng Liu ,  Tiantian Liu

IPC分类号： C12P7/56 ,  C12N1/18 ,  C12N9/04 ,  C12N15/90 ,  C12R1/865

CPC分类号： C12P7/56 ,  C12N1/185 ,  C12N9/0006 ,  C12N15/905 ,  C12Y101/01027 ,  C12Y101/05005 ,  C12R2001/865

摘要： The present disclosure discloses a method for improving production of L-lactic acid (L-LA) by Saccharomyces cerevisiae based on regulation and control of ethanol metabolic flux, and belongs to the technical field of microorganisms. According to the present disclosure, acid-resistant Saccharomyces cerevisiae TJG16 is used as a production strain, an ethanol dehydrogenase gene adhA derived from Bacillus subtilis is introduced to promote conversion of ethanol into acetaldehyde, and a lactate aldolase gene BAL derived from Brucella sp. is introduced to promote synthesis of lactic acid from the acetaldehyde. Moreover, an acetaldehyde dehydrogenase gene ALD6 is knocked out to prevent synthesis of acetic acid from the acetaldehyde, a transcriptional regulatory factor encoding gene GAL80 for regulating and controlling galactose is knocked out, and lactate dehydrogenase LDH is integrated, so that the L-LA is finally increased.

公开(公告)号：US20240067996A1

公开(公告)日：2024-02-29

申请号：US18499560

申请日：2023-11-01

申请人： Jiangnan University ,  Inner Mongolia Mengniu Dairy (Group) Company Limited

发明人： Long LIU ,  Jian Chen ,  Xueqin LV ,  Wenyang Wu ,  Guocheng Du ,  Jianghua Li ,  Guolin Zhou ,  Yanfeng Liu ,  Chenyang Zhang

IPC分类号： C12P7/6409 ,  C12N9/10 ,  C12N9/20 ,  C12N15/81

CPC分类号： C12P7/6409 ,  C12N9/1029 ,  C12N9/20 ,  C12N15/81 ,  C12Y203/01051 ,  C12Y301/01003 ,  C12N2800/102

摘要： The invention provides a Saccharomyces cerevisiae strain for producing a human milk lipid substitute. By integrating a heterologous lysophosphatidic acid acyltransferase into Saccharomyces cerevisiae and knocking out its own natural lysophosphatidic acid acyltransferase, the content of palmitic acid (C16:0) at Sn-2 position of triacylglycerol produced by Saccharomyces cerevisiae is increased, to synthesize a human milk lipid substitute. On this basis, a metabolic pathway related gene is knocked out, to further increase the content of human milk lipid substitute in the product. In the present invention, a human milk lipid substitute is de novo synthesized by Saccharomyces cerevisiae for the first time, in which the total fatty acid is 15% or more, and the relative content of C16:0 at Sn-2 position reaches about 60%.

公开(公告)号：US10975377B2

公开(公告)日：2021-04-13

申请号：US16255054

申请日：2019-01-23

申请人： Jiangnan University

发明人： Yanfeng Liu ,  Guocheng Du ,  Rongzhen Tian ,  Jian Chen

IPC分类号： C12N15/75 ,  C07K14/435

摘要： The present disclosure relates to a method for regulating expression of protein of interest in Bacillus subtilis, and belongs to the technical field of genetic engineering. The method comprises: using Bacillus subtilis as an expression host, adding the N-terminal nucleotide sequence coding the first 15 amino acids of the endogenous protein before the coding gene of the protein of interest or modifying the original N-terminal nucleotide sequence coding the first 15 amino acids, and performing free expression in plasmid, thereby regulating expression of the protein of interest in Bacillus subtilis, and even regulating the expression difference in different growth phases and the expression level.

公开(公告)号：US11618902B2

公开(公告)日：2023-04-04

申请号：US17176313

申请日：2021-02-16

申请人： Jiangnan University

发明人： Yanfeng Liu ,  Long Liu ,  Xiaolong Zhang ,  Guocheng Du ,  Jianghua Li ,  Jian Chen

IPC分类号： C12N1/20 ,  C12N15/75 ,  C12P19/26 ,  C12R1/25

摘要： The disclosure discloses Bacillus subtilis for producing N-acetylneuraminic acid and application thereof, and belongs to the field of genetic engineering. The disclosure optimizes the expression levels of key enzymes in N-acetylneuraminic acid synthesis pathways on genome through promoters of different strength, reduces the protein synthesis pressure caused by the expression of enzymes on cells, and further integrates the three N-acetylneuraminic acids in a same Bacillus subtilis engineering strain. Bacillus subtilis with improved N-acetylneuraminic acid production is obtained, and the production reaches 10.4 g/L at the shake flask level, laying a foundation for further improving the NeuAc production from Bacillus subtilis.