公开(公告)号：US20220389417A1

公开(公告)日：2022-12-08

申请号：US17829316

申请日：2022-05-31

申请人： Inscripta, Inc.

发明人： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC分类号： C12N15/11 ,  C12N15/90 ,  C12N9/22

摘要： Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US11408012B2

公开(公告)日：2022-08-09

申请号：US17588256

申请日：2022-01-29

申请人： Inscripta, Inc.

发明人： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC分类号： C12N15/90 ,  C12N9/22 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/11 ,  C12N15/10 ,  C12N15/63 ,  C12N15/113

摘要： Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20220162646A1

公开(公告)日：2022-05-26

申请号：US17588256

申请日：2022-01-29

申请人： Inscripta, Inc.

发明人： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC分类号： C12N15/90 ,  C12N9/22 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/11 ,  C12N15/10 ,  C12N15/63 ,  C12N15/113

摘要： Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US11220697B2

公开(公告)日：2022-01-11

申请号：US17387860

申请日：2021-07-28

申请人： Inscripta, Inc.

发明人： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC分类号： C12N15/90 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N9/22 ,  C12N15/11

摘要： Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US10017760B2

公开(公告)日：2018-07-10

申请号：US15632222

申请日：2017-06-23

申请人： The Regents of the University of Colorado, a body corporate ,  Inscripta, Inc.

发明人： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC分类号： C12N15/09 ,  C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12N15/64 ,  C12N15/70 ,  C12N15/74 ,  C12N15/79 ,  C12N15/81 ,  C12N15/82 ,  C12N15/85

CPC分类号： C12N15/1065 ,  C12N15/102 ,  C12N15/1079 ,  C12N15/1082 ,  C12N15/11 ,  C12N2310/20 ,  C12Q2563/179

摘要： Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US20230227810A1

公开(公告)日：2023-07-20

申请号：US18157740

申请日：2023-01-20

申请人： Inscripta, Inc.

发明人： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC分类号： C12N15/10 ,  C12N15/11

CPC分类号： C12N15/1065 ,  C12N15/11 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/1079 ,  C12N2310/20

摘要： Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US11130970B2

公开(公告)日：2021-09-28

申请号：US17179193

申请日：2021-02-18

申请人： Inscripta, Inc.

发明人： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC分类号： C12N15/90 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N9/22 ,  C12N15/11

摘要： Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20190194650A1

公开(公告)日：2019-06-27

申请号：US16295393

申请日：2019-03-07

申请人： The Regents of the University of Colorado, a body corporate ,  Inscripta, Inc.

发明人： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC分类号： C12N15/10 ,  C12N15/11

CPC分类号： C12N15/1065 ,  C12N15/102 ,  C12N15/1079 ,  C12N15/1082 ,  C12N15/11 ,  C12N2310/20 ,  C12Q2563/179

摘要： Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US10287575B2

公开(公告)日：2019-05-14

申请号：US15948798

申请日：2018-04-09

申请人： The Regents of the University of Colorado ,  Inscripta, Inc.

发明人： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC分类号： C12N15/10 ,  C12N15/11

摘要： Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US09982279B1

公开(公告)日：2018-05-29

申请号：US15632001

申请日：2017-06-23

申请人： Inscripta, Inc.

发明人： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb

IPC分类号： C12N15/90 ,  C12N15/11 ,  C12N15/10 ,  C12N15/85 ,  C12N9/22 ,  C12N5/10 ,  C12N5/14 ,  C12N5/16 ,  C12N5/22 ,  C12N15/09 ,  C12N15/52 ,  C12N15/63 ,  C12N15/79 ,  C12N15/70 ,  C12N15/81

CPC分类号： C12N15/902 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/905 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/22

摘要： Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.