公开(公告)号：US11787843B2

公开(公告)日：2023-10-17

申请号：US17178179

申请日：2021-02-17

Applicant: Synthetic Genomics, Inc.

Inventor： Eric R. Moellering ,  Nicholas Bauman ,  Randor R. Radakovits ,  Roberto Spreafico ,  Fedor Kuzminov ,  Imad Ajjawi ,  Saheed Imam ,  Andrew Schultz ,  Kathleen Kwok ,  Moena Aqui ,  Jennifer Nominati ,  John Verruto ,  Shaun Bailey

IPC: C12N1/12 ,  C07K14/405 ,  C12N1/36 ,  C12N15/82 ,  C12P21/00 ,  C12N15/01 ,  C12P7/6445 ,  A01H1/06 ,  C12P7/649 ,  C12P7/6454 ,  C12R1/89

CPC classification number: C07K14/405 ,  A01H1/06 ,  C12N1/12 ,  C12N1/125 ,  C12N1/36 ,  C12N15/01 ,  C12N15/8269 ,  C12P7/649 ,  C12P7/6445 ,  C12P7/6454 ,  C12P21/00 ,  C12R2001/89 ,  Y02E50/10

Abstract: Disclosed herein are mutant photosynthetic microorganisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.

公开(公告)号：US20210348183A1

公开(公告)日：2021-11-11

申请号：US17307824

申请日：2021-05-04

Applicant: Synthetic Genomics, Inc.

Inventor： John H. Verruto ,  Jessica N. Weir

IPC: C12N15/82 ,  C12Q1/6806 ,  C12N9/10 ,  C12N1/12

Abstract: The invention provides methods of transforming photosynthetic organisms, such as green algae. The methods involve methylating one or more DNA fragments of a DNA construct and transforming the one or more fragments into the photosynthetic organism. The DNA fragments can be the product of a DNA amplification procedure, such as PCR or a PCR-like procedure. In one embodiment the one or more fragments of DNA that comprise a DNA construct are dam methylated prior to being transformed into the photosynthetic organism.

公开(公告)号：US20210017530A1

公开(公告)日：2021-01-21

申请号：US17039839

申请日：2020-09-30

Applicant: Synthetic Genomics, Inc.

Inventor： John Verruto ,  Eric Moellering

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/85 ,  C12N15/90 ,  C12N9/12

Abstract: The present invention provides cell lines for high efficiency genome editing using cas/CRISPR systems, methods of generating such cells lines and methods of generating mutations in the genome of an organism using such cell lines.

公开(公告)号：US10683514B2

公开(公告)日：2020-06-16

申请号：US16509095

申请日：2019-07-11

Applicant: Synthetic Genomics, Inc.

Inventor： Nicholas Bauman ,  Imad Ajjawi

IPC: C12N15/82 ,  C07K14/405 ,  C12P7/64

Abstract: The application provides recombinant microorganisms with increased productivity with respect to control or wildtype microorganisms. The recombinant microorganisms can include a non-native gene encoding a SKP1 polypeptide or a CHORD-derived polypeptide. Increased productivity can be increased biomass or lipid productivity. These recombinant microorganisms can be used to produce products of interest.

公开(公告)号：US10563232B2

公开(公告)日：2020-02-18

申请号：US16267940

申请日：2019-02-05

Applicant: Synthetic Genomics, Inc.

Inventor： Imad Ajjawi ,  Eric R. Moellering ,  Leah Soriaga ,  Moena Aqui

IPC: C12N1/12 ,  C12P7/64 ,  C07K14/405

Abstract: The present invention provides mutant microorganism that have higher lipid productivity than the wild type microorganisms from which they are derived while biomass at levels that are within approximately 50% of wild type biomass productivities under nitrogen replete conditions. Particular mutants produce at least twice as much FAME lipid as wild type while producing at least 75% of the biomass produced by wild type cells under nitrogen replete conditions. Also provided are methods of producing lipid using the mutant strains.

公开(公告)号：US20200046848A1

公开(公告)日：2020-02-13

申请号：US16609420

申请日：2018-05-01

Applicant: LAWRENCE LIVERMORE NATIONAL SECURITY, LLC ,  SYNTHETIC GENOMICS, INC.

Inventor： Matthew A. COLEMAN ,  Nicholas O. FISCHER ,  Amy RASLEY ,  Craig D. BLANCHETTE ,  Todd PETERSON

IPC: A61K47/69 ,  A61K9/127 ,  A61K9/00 ,  A61K39/02 ,  A61P31/04 ,  A61K39/39 ,  B82Y5/00

Abstract: A telodendrimer-nanolipoprotein particle (t-NLP), comprising one or more membrane forming lipids, one or more telodendrimers, and a scaffold protein and a Chlamydia major outer membrane protein (MOMP) comprising a MOMP hydrophobic region, and related compositions methods and systems.

公开(公告)号：US20200024623A1

公开(公告)日：2020-01-23

申请号：US16518360

申请日：2019-07-22

Applicant: SYNTHETIC GENOMICS, INC.

Inventor： ERIC R. MOELLERING ,  TOM CARLSON

IPC: C12P7/64 ,  C12N9/04 ,  C12N9/88 ,  C12N9/02

Abstract: The present invention provides a mutant algal microorganism that has a mutation that causes attenuated expression of TrifuncB and/or TrifuncA and as a result produces more lipids than a control algal microorganism. The mutant algal microorganism can further include a mutation in a gene encoding a peroxisomal beta-oxidation pathway protein, such as an ACO1 or PXA1 gene, or a glyoxylate pathway protein, such as an ICL1 gene, that results in attenuated expression and further increased lipid production. Furthermore, provided herein are methods of producing lipids using the mutant algal microorganisms and methods of making the mutant microorganisms.

公开(公告)号：US20190241921A1

公开(公告)日：2019-08-08

申请号：US16388722

申请日：2019-04-18

Applicant: Synthetic Genomics, Inc.

Inventor： Daniel G. Gibson ,  Hamilton O. Smith ,  Clyde A. Hutchison ,  Lei Young ,  J. Craig Venter

IPC: C12P19/34 ,  C12N15/66 ,  C12N15/10 ,  C12N15/64

CPC classification number: C12P19/34 ,  C12N15/10 ,  C12N15/1027 ,  C12N15/64 ,  C12N15/66

Abstract: The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization.

公开(公告)号：US20190203221A1

公开(公告)日：2019-07-04

申请号：US16234209

申请日：2018-12-27

Applicant: Synthetic Genomics, Inc.

Inventor： Imad Ajjawi ,  Fedor I. Kuzminov ,  Randor R. Radakovits ,  John H. Verruto ,  Sarah Tacke ,  Roberto Spreafico ,  William F. Lambert ,  Jessica Nichole Greiner

IPC: C12N15/82 ,  C12N15/62 ,  A61K36/02 ,  C12N15/10

CPC classification number: C12N15/8269 ,  A61K36/02 ,  C12N15/102 ,  C12N15/62 ,  C12N15/8201 ,  C12N15/8222 ,  C12N15/8243

Abstract: Mutant photosynthetic organisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutant strains have mutated or attenuated: chloroplastic SRP54 gene and SGI1 gene; chloroplastic SRP54 gene and SGI2 gene; chloroplastic SRP54 gene, SGI1, and SGI2 genes are disclosed. The mutant photosynthetic organisms exhibit increased productivity with respect to wild-type strains. Also provided are mutant photosynthetic organisms having mutated or attenuated cytosolic SRP54 genes. Provided herein are methods of producing biomass and other products such as lipids using strains having mutations in an SRP54 gene, SGI1, SGI2 genes, a combination of SGI1/SRP54, and a combination of SGI2 and SRP54 genes. Also included are constructs and methods for attenuating or disrupting SRP54, SGI1, and SGI2 genes.

公开(公告)号：US20190177759A1

公开(公告)日：2019-06-13

申请号：US16212534

申请日：2018-12-06

Applicant: SYNTHETIC GENOMICS, INC.

Inventor： ZHIQING Qi ,  Jun Urano ,  Nicky C. Caiazza ,  Daniel G. Gibson

IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/6844

Abstract: The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a DNA polymerase, a mixture of dNTPs, and a crowding agent to form an assembly mixture. In one embodiment the crowding agent is polyethylene glycol (PEG). The presence of the crowding agent facilitates the nucleic acid assembly process of the invention. The assembly mixture is then subjected to multiple cycles, each cycle comprising an annealing phase, an extension phase, and a denaturation phase, and the desired nucleic acid molecule is thereby assembled. In some embodiments one or more of the phases are time varied.