知搜-全球专利检索

  1. Login
  2. Sign Up

Search Results

12 records (took 0.021 seconds)

    REGULATORY ELEMENTS AND USES THEREOF
    3.
    发明申请
    REGULATORY ELEMENTS AND USES THEREOF 有权
    法规要素及其用途

    公开(公告)号：US20140363892A1

    公开(公告)日：2014-12-11

    申请号：US13915522

    申请日：2013-06-11

    Applicant: SYNTHETIC GENOMICS, INC.

    Inventor： Michele M. Champagne ,  Toby H. Richardson ,  Jun Urano

    IPC: C12N15/82

    CPC classification number: C12N15/8216 ,  C07K14/405 ,  C12N15/79

    Abstract: The present application provides novel regulatory elements including promoter sequences from marine microorganisms. The application further discloses DNA constructs containing these novel regulatory elements; transgenic cells, transgenic non-human organisms, and progeny containing these novel regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. The regulatory elements disclosed herein are particularly suited for use in Nannochloropsis and other microalgae.

    Abstract translation: 本申请提供了新的调控元件，包括来自海洋微生物的启动子序列。 本申请进一步公开了含有这些新型调节元件的DNA构建体; 转基因细胞，转基因非人生物和含有这些新型调节元件的后代。 还公开了修饰，制备和使用调节元件的方法。 本文公开的调节元件特别适用于南非氯氰菊酯和其他微藻类。

    PEG-MEDIATED ASSEMBLY OF NUCLEIC ACID MOLECULES
    4.
    发明申请
    PEG-MEDIATED ASSEMBLY OF NUCLEIC ACID MOLECULES 审中-公开

    公开(公告)号：US20190177759A1

    公开(公告)日：2019-06-13

    申请号：US16212534

    申请日：2018-12-06

    Applicant: SYNTHETIC GENOMICS, INC.

    Inventor： ZHIQING Qi ,  Jun Urano ,  Nicky C. Caiazza ,  Daniel G. Gibson

    IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/6844

    Abstract: The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a DNA polymerase, a mixture of dNTPs, and a crowding agent to form an assembly mixture. In one embodiment the crowding agent is polyethylene glycol (PEG). The presence of the crowding agent facilitates the nucleic acid assembly process of the invention. The assembly mixture is then subjected to multiple cycles, each cycle comprising an annealing phase, an extension phase, and a denaturation phase, and the desired nucleic acid molecule is thereby assembled. In some embodiments one or more of the phases are time varied.

    Regulatory elements from labyrinthulomycetes microorganisms
    5.
    发明授权
    Regulatory elements from labyrinthulomycetes microorganisms 有权

    公开(公告)号：US09932599B2

    公开(公告)日：2018-04-03

    申请号：US15056857

    申请日：2016-02-29

    Applicant: Synthetic Genomics, Inc.

    Inventor： Nicky C. Caiazza ,  Maung Nyan Win ,  Jun Urano

    IPC: C12N15/79 ,  C12N15/63 ,  C12N15/80

    CPC classification number: C12N15/79 ,  C12N15/63 ,  C12N15/80

    Abstract: The present disclosure generally relates to novel polynucleotide molecules for use in regulating gene expression in recombinant cells, such as labyrinthulomycetes cells. The disclosure further relates to nucleic acid constructs, such as vectors and expression cassettes, containing a regulatory element operably linked to a heterologous nucleotide sequence. The disclosure further relates to methods for stably transforming a host cell, such as a labyrinthulomycetes cell with transgenes. Stably transformed recombinant cells, progeny, biomaterials derived therefrom, and methods for preparing and using the same are also provided.

    PEG-MEDIATED ASSEMBLY OF NUCLEIC ACID MOLECULES
    6.
    发明申请
    PEG-MEDIATED ASSEMBLY OF NUCLEIC ACID MOLECULES 审中-公开
    PEG介导的核酸分子组装

    公开(公告)号：US20140308710A1

    公开(公告)日：2014-10-16

    申请号：US14103578

    申请日：2013-12-11

    Applicant: Synthetic Genomics, Inc.

    Inventor： Zhiqing Qi ,  Jun Urano ,  Nicky C. Caiazza ,  Daniel G. Gibson

    IPC: C12P19/34

    CPC classification number: C12P19/34 ,  C12N15/1027 ,  C12N15/1031 ,  C12Q1/6844 ,  C12Q2521/101 ,  C12Q2527/101 ,  C12Q2527/125 ,  C12Q2527/143 ,  C12Q2533/107

    Abstract: The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a DNA polymerase, a mixture of dNTPs, and a crowding agent to form an assembly mixture. In one embodiment the crowding agent is polyethylene glycol (PEG). The presence of the crowding agent facilitates the nucleic acid assembly process of the invention. The assembly mixture is then subjected to multiple cycles, each cycle comprising an annealing phase, an extension phase, and a denaturation phase, and the desired nucleic acid molecule is thereby assembled. In some embodiments one or more of the phases are time varied.

    Abstract translation: 本发明公开了从一组重叠寡核苷酸组装核酸分子的方法。 该方法包括将一组重叠的寡核苷酸与DNA聚合酶，dNTP的混合物和拥挤剂接触以形成组合混合物。 在一个实施方案中，拥挤剂是聚乙二醇（PEG）。 拥挤剂的存在促进了本发明的核酸组装过程。 然后将组合混合物进行多个循环，每个循环包含退火阶段，延伸相和变性阶段，从而组装所需的核酸分子。 在一些实施方案中，一个或多个相是时间变化的。

    REGULATORY ELEMENTS FROM LABYRINTHULOMYCETES MICROORGANISMS
    7.
    发明申请
    REGULATORY ELEMENTS FROM LABYRINTHULOMYCETES MICROORGANISMS 审中-公开

    公开(公告)号：US20180201941A1

    公开(公告)日：2018-07-19

    申请号：US15916171

    申请日：2018-03-08

    Applicant: Synthetic Genomics, Inc.

    Inventor： Nicky C. Caiazza ,  Maung Nyan Win ,  Jun Urano

    IPC: C12N15/79 ,  C12N15/80 ,  C12N15/63

    CPC classification number: C12N15/79 ,  C12N15/63 ,  C12N15/80

    Abstract: The present disclosure generally relates to novel polynucleotide molecules for use in regulating gene expression in recombinant cells, such as labyrinthulomycetes cells. The disclosure further relates to nucleic acid constructs, such as vectors and expression cassettes, containing a regulatory element operably linked to a heterologous nucleotide sequence. The disclosure further relates to methods for stably transforming a host cell, such as a labyrinthulomycetes cell with transgenes. Stably transformed recombinant cells, progeny, biomaterials derived therefrom, and methods for preparing and using the same are also provided.

    Regulatory elements and uses thereof
    8.
    发明授权
    Regulatory elements and uses thereof 有权
    调节元素及其用途

    公开(公告)号：US09546372B2

    公开(公告)日：2017-01-17

    申请号：US13915522

    申请日：2013-06-11

    Applicant: SYNTHETIC GENOMICS, INC.

    Inventor： Michele M. Champagne ,  Toby H. Richardson ,  Jun Urano

    IPC: C12N15/82 ,  C12N15/79

    CPC classification number: C12N15/8216 ,  C07K14/405 ,  C12N15/79

    Abstract: The present application provides novel regulatory elements including promoter sequences from marine microorganisms. The application further discloses DNA constructs containing these novel regulatory elements; transgenic cells, transgenic non-human organisms, and progeny containing these novel regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. The regulatory elements disclosed herein are particularly suited for use in Nannochloropsis and other microalgae.

    Abstract translation: 本申请提供了新的调控元件，包括来自海洋微生物的启动子序列。 本申请进一步公开了含有这些新型调节元件的DNA构建体; 转基因细胞，转基因非人生物和含有这些新型调节元件的后代。 还公开了修饰，制备和使用调节元件的方法。 本文公开的调节元件特别适用于南非氯氰菊酯和其他微藻类。

    Molecules Associated with Fatty Acid Biosynthetic Pathways and Uses Thereof
    9.
    发明申请
    Molecules Associated with Fatty Acid Biosynthetic Pathways and Uses Thereof 审中-公开
    与脂肪酸生物合成途径相关的分子及其用途

    公开(公告)号：US20160122741A1

    公开(公告)日：2016-05-05

    申请号：US14637356

    申请日：2015-03-03

    Applicant: Synthetic Genomics, Inc.

    Inventor： Gerardo V. Toledo ,  Karen D. Xu ,  Jun Urano

    IPC: C12N9/00 ,  C12N15/79 ,  C12P7/64

    CPC classification number: C12N9/93 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/6427 ,  C12Y604/00

    Abstract: The present disclosure relates in part to recombinant microorganisms that include non-native genes encoding PUFA-PKS polypeptides, and to methods of making and using such microorganisms for producing at least one PUFA. In particular, the disclosure further relates to methods and related materials useful for the production of at least one PUFA by heterologous expression of the nucleic acid sequences disclosed herein encoding PUFA-PKS polypeptides.

    Abstract translation: 本公开部分涉及包含编码PUFA-PKS多肽的非天然基因的重组微生物，以及制备和使用这些微生物以产生至少一种PUFA的方法。 具体地，本公开进一步涉及可用于通过异源表达本文公开的编码PUFA-PKS多肽的核酸序列来生产至少一种PUFA的方法和相关材料。

Patent Agency Ranking