公开(公告)号：US20200017907A1

公开(公告)日：2020-01-16

申请号：US16417023

申请日：2019-05-20

Applicant: Twist Bioscience Corporation

Inventor： Ramsey Ibrahim ZEITOUN ,  Siyuan CHEN ,  Richard GANTT ,  Kristin D. BUTCHER ,  E. Hutson CHILTON

IPC: C12Q1/6869 ,  G16B40/10 ,  C40B30/10 ,  C40B70/00

Abstract: Provided herein are compositions, methods and systems relating to libraries of polynucleotides such that the libraries allow for accurate and efficient hybridization after binding to target sequences. Further provided herein are probes, blockers, additives, buffers, and methods that result in improved hybridization. Such compositions and methods are useful for improvement of Next Generation Sequencing applications, such as reducing off-target binding or reducing workflow times.

公开(公告)号：US20160310927A1

公开(公告)日：2016-10-27

申请号：US15135434

申请日：2016-04-21

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William BANYAI ,  Bill James PECK ,  Andres FERNANDEZ ,  Siyuan CHEN ,  Pierre INDERMUHLE ,  Eugene P. MARSH

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00497 ,  B01J2219/00511 ,  B01J2219/00522 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01L3/50857 ,  C12N15/1093 ,  C12Q2563/159 ,  C12Q2565/513 ,  C12Q2565/629

Abstract: Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.

Abstract translation: 本文提供了用于从头合成大量和高精度的寡核酸文库的装置和方法。 器件包括具有主通道和微通道的结构，其中微通道具有高表面积与体积比。 本文公开的装置提供具有低错误率的寡核酸的从头合成。

公开(公告)号：US20160251651A1

公开(公告)日：2016-09-01

申请号：US15156134

申请日：2016-05-16

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William BANYAI ,  Bill James PECK ,  Andres FERNANDEZ ,  Siyuan CHEN ,  Pierre INDERMUHLE

IPC: C12N15/10

CPC classification number: C12N15/1093 ,  C12Q1/6806 ,  C12Q2521/501 ,  C12Q2525/179 ,  C12Q2525/191 ,  C12Q2525/204 ,  C12Q2525/307 ,  C12Q2527/143 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/626 ,  C12Q2531/125

Abstract: Methods and devices for cell-free sorting and cloning of nucleic acid libraries are provided herein.

Abstract translation: 本文提供了用于无细胞分选和克隆核酸文库的方法和装置。

公开(公告)号：US20180355351A1

公开(公告)日：2018-12-13

申请号：US16006581

申请日：2018-06-12

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Rebecca NUGENT ,  Siyuan CHEN ,  Elian LEE ,  Nathan RAYNARD

IPC: C12N15/10 ,  C12N9/00 ,  C12N9/12 ,  C12P19/34 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.

公开(公告)号：US20170159044A1

公开(公告)日：2017-06-08

申请号：US15433909

申请日：2017-02-15

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Esteban Toro ,  Sebastian TREUSCH ,  Siyuan CHEN ,  Cheng-Hsien WU

IPC: C12N15/10 ,  C12P19/34

CPC classification number: C12N15/1031 ,  C12N15/1027 ,  C12N15/1093 ,  C12N15/66 ,  C12P19/34

Abstract: Methods and compositions are provided for assembly of large nucleic acids where the assembled large nucleic acids lack internal sequence modifications made during the assembly process.

公开(公告)号：US20160354752A1

公开(公告)日：2016-12-08

申请号：US15245054

申请日：2016-08-23

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William BANYAI ,  Bill James PECK ,  Andres FERNANDEZ ,  Siyuan CHEN ,  Pierre INDERMUHLE

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00623 ,  B01J2219/00637 ,  B01J2219/00709 ,  B01J2219/00722 ,  C12N15/09 ,  C12N15/1093 ,  C12N15/1096 ,  C12N15/66 ,  C12N15/74 ,  C40B40/06 ,  C40B50/00 ,  C40B50/14 ,  C40B50/18

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

公开(公告)号：US20160096160A1

公开(公告)日：2016-04-07

申请号：US14885963

申请日：2015-10-16

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William BANYAI ,  Bill James PECK ,  Andres FERNANDEZ ,  Siyuan CHEN ,  Pierre INDERMUHLE

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00623 ,  B01J2219/00637 ,  B01J2219/00709 ,  B01J2219/00722 ,  C12N15/09 ,  C12N15/1093 ,  C12N15/1096 ,  C12N15/66 ,  C12N15/74 ,  C40B40/06 ,  C40B50/00 ,  C40B50/14 ,  C40B50/18

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

公开(公告)号：US20240158955A1

公开(公告)日：2024-05-16

申请号：US18356787

申请日：2023-07-21

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Anthony COX ,  Sebastian TREUSCH ,  Siyuan CHEN

IPC: C40B50/06 ,  C12N15/10 ,  C12N15/113 ,  C12Q1/6876 ,  C40B50/08 ,  C40B50/14 ,  G01N33/50

CPC classification number: C40B50/06 ,  C12N15/1079 ,  C12N15/1093 ,  C12N15/113 ,  C12Q1/6876 ,  C40B50/08 ,  C40B50/14 ,  G01N33/502 ,  G01N33/5029 ,  G01N33/5041 ,  C12N2320/30 ,  C12N2330/31 ,  C12Q1/6886

Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.

公开(公告)号：US20240043920A1

公开(公告)日：2024-02-08

申请号：US18341544

申请日：2023-06-26

Applicant: Twist Bioscience Corporation

Inventor： Ramsey Ibrahim ZEITOUN ,  Siyuan CHEN ,  Richard GANTT ,  Kristin D. BUTCHER ,  E. Hutson CHILTON

IPC: C12Q1/6869 ,  G16B40/10 ,  C40B30/10 ,  C40B70/00

CPC classification number: C12Q1/6869 ,  G16B40/10 ,  C40B30/10 ,  C40B70/00

Abstract: Provided herein are compositions, methods and systems relating to libraries of polynucleotides such that the libraries allow for accurate and efficient hybridization after binding to target sequences. Further provided herein are probes, blockers, additives, buffers, and methods that result in improved hybridization. Such compositions and methods are useful for improvement of Next Generation Sequencing applications, such as reducing off-target binding or reducing workflow times.

公开(公告)号：US20230338913A1

公开(公告)日：2023-10-26

申请号：US18319356

申请日：2023-05-17

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William BANYAI ,  Bill James PECK ,  Andres FERNANDEZ ,  Siyuan CHEN ,  Pierre INDERMUHLE ,  Eugene P. MARSH

IPC: B01J19/00 ,  C12N15/10

CPC classification number: B01J19/0046 ,  C12N15/1093 ,  B01J2219/00596 ,  B01J2219/00722 ,  B01J2219/00497 ,  B01J2219/00585 ,  B01J2219/00317 ,  B01J2219/00511 ,  B01J2219/00522 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00662 ,  B01L3/50857

Abstract: Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.