公开(公告)号：US20180237851A1

公开(公告)日：2018-08-23

申请号：US15953822

申请日：2018-04-16

Applicant: Karius, Inc.

Inventor： Fred C. Christians ,  Igor D. Vilfan ,  Michael Kertesz ,  Timothy A. Blauwkamp ,  Shivkumar Venkatasubrahmanyam ,  Michael Rosen ,  Rene Sit

IPC: C12Q1/6874 ,  C12Q1/689 ,  C12N15/10 ,  G06F19/22

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6869 ,  C12Q1/689 ,  C12Q2600/166 ,  G16B30/00 ,  C12Q2545/101

Abstract: This disclosure provides methods for determining relative abundance of one or more non-host species in a sample from a host. Also provided are methods involving addition of known concentrations of synthetic nucleic acids to a sample and performing sequencing assays to identify non-host species such as pathogens. Also provided are methods of tracking samples, tracking reagents, and tracking diversity loss in sequencing assays.

公开(公告)号：US20170016048A1

公开(公告)日：2017-01-19

申请号：US15157374

申请日：2016-05-17

Applicant: Karius, Inc.

Inventor： Timothy A. Blauwkamp ,  Fred Christians ,  Igor D. Vilfan ,  Scott Smith ,  Michael Kertesz

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6888 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2537/159

Abstract: This disclosure provides methods and compositions that are useful for enriching a particular population of nucleic acids (a “population of interest”) within a complex mixture of nucleic acids. The population of interest may make up a minor portion of a complex mixture of nucleic acids. The methods and compositions provided herein are useful for detecting, predicting, diagnosing, or monitoring a disease or disorder, particularly a disease or disorder caused by a foreign microbe or pathogen.

Abstract translation: 本公开提供了可用于在核酸的复杂混合物中富集特定的核酸群体（“感兴趣群体”）的方法和组合物。 感兴趣的群体可能构成复杂的核酸混合物的一小部分。 本文提供的方法和组合物可用于检测，预测，诊断或监测疾病或病症，特别是由外来微生物或病原体引起的疾病或病症。

公开(公告)号：US20240294979A1

公开(公告)日：2024-09-05

申请号：US18486005

申请日：2023-10-12

Applicant: Karius, Inc.

Inventor： Timothy A. Blauwkamp ,  Rene Sit ,  Igor D. Vilfan

IPC: C12Q1/6869 ,  C12N9/00 ,  C12N9/12 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6876

CPC classification number: C12Q1/6869 ,  C12N9/1247 ,  C12N9/93 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q2521/107 ,  C12Q2533/101 ,  C12Y207/07006 ,  C12Y207/07049 ,  C12Y605/01001 ,  C12Y605/01003

Abstract: The disclosure provides methods, compositions, systems, and kits for the concurrent detection and analysis of different structural and chemical forms of nucleic acids in a sample.

公开(公告)号：US11180800B2

公开(公告)日：2021-11-23

申请号：US15930488

申请日：2020-05-13

Applicant: Karius, Inc.

Inventor： Timothy A. Blauwkamp ,  Rene Sit ,  Igor D. Vilfan

IPC: C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12N9/12 ,  C12N9/00 ,  C12Q1/6876

Abstract: The disclosure provides methods, compositions, systems, and kits for the concurrent detection and analysis of different structural and chemical forms of nucleic acids in a sample.

公开(公告)号：US20170275691A1

公开(公告)日：2017-09-28

申请号：US15469474

申请日：2017-03-24

Applicant: Karius, Inc.

Inventor： Fred C. Christians ,  Igor D. Vilfan ,  Michael Kertesz ,  Timothy A. Blauwkamp ,  Shivkumar Venkatasubrahmanyam ,  Michael Rosen ,  Rene Sit

IPC: C12Q1/68 ,  G06F19/22 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6869 ,  C12Q1/689 ,  C12Q2600/166 ,  G06F19/22 ,  C12Q2545/101

Abstract: This disclosure provides methods for determining relative abundance of one or more non-host species in a sample from a host. Also provided are methods involving addition of known concentrations of synthetic nucleic acids to a sample and performing sequencing assays to identify non-host species such as pathogens. Also provided are methods of tracking samples, tracking reagents, and tracking diversity loss in sequencing assays.

公开(公告)号：US20240309427A1

公开(公告)日：2024-09-19

申请号：US18674513

申请日：2024-05-24

Applicant: Karius, Inc.

Inventor： Timothy A. Blauwkamp ,  Fred Christians ,  Michael Rosen ,  Igor D. Vilfan

IPC: C12Q1/6806 ,  C12N15/10 ,  C12Q1/6848 ,  C12Q1/6869 ,  C12Q1/6876

CPC classification number: C12Q1/6806 ,  C12N15/10 ,  C12N15/1003 ,  C12Q1/6848 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2523/313

Abstract: This disclosure provides methods that are useful for reducing or inactivating contaminant nucleic acids.

公开(公告)号：US20210324467A1

公开(公告)日：2021-10-21

申请号：US17355882

申请日：2021-06-23

Applicant: Karius, Inc.

Inventor： Fred C. Christians ,  Igor D. Vilfan ,  Michael Kertesz ,  Timothy A. Blauwkamp ,  Shivkumar Venkatasubrahmanyam ,  Michael Rosen ,  Rene Sit

IPC: C12Q1/6874 ,  G16B30/00 ,  C12Q1/6869 ,  G16B30/10 ,  C12N15/10 ,  C12Q1/689

Abstract: This disclosure provides methods for determining relative abundance of one or more non-host species in a sample from a host. Also provided are methods involving addition of known concentrations of synthetic nucleic acids to a sample and performing sequencing assays to identify non-host species such as pathogens. Also provided are methods of tracking samples, tracking reagents, and tracking diversity loss in sequencing assays.

公开(公告)号：US10697008B2

公开(公告)日：2020-06-30

申请号：US15952203

申请日：2018-04-12

Applicant: Karius, Inc.

Inventor： Timothy A. Blauwkamp ,  Rene Sit ,  Igor D. Vilfan

IPC: C12Q1/6869 ,  C12Q1/6806 ,  C12N9/12 ,  C12N9/00 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6876 ,  C12N1/00

Abstract: The disclosure provides methods, compositions, systems, and kits for the concurrent detection and analysis of different structural and chemical forms of nucleic acids in a sample.

公开(公告)号：US20180371538A1

公开(公告)日：2018-12-27

申请号：US15952203

申请日：2018-04-12

Applicant: Karius, Inc.

Inventor： Timothy A. Blauwkamp ,  Rene Sit ,  Igor D. Vilfan

IPC: C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12N9/12 ,  C12N9/00 ,  C12Q1/686

Abstract: The disclosure provides methods, compositions, systems, and kits for the concurrent detection and analysis of different structural and chemical forms of nucleic acids in a sample.

公开(公告)号：US20250034639A1

公开(公告)日：2025-01-30

申请号：US18916558

申请日：2024-10-15

Applicant: Karius, Inc.

Inventor： Fred C. Christians ,  Igor D. Vilfan ,  Michael Kertesz ,  Timothy A. Blauwkamp ,  Shivkumar Venkatasubrahmanyam ,  Michael Rosen ,  Rene Sit

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/6869 ,  C12Q1/689 ,  G16B30/00 ,  G16B30/10

Abstract: This disclosure provides methods for determining relative abundance of one or more non-host species in a sample from a host. Also provided are methods involving addition of known concentrations of synthetic nucleic acids to a sample and performing sequencing assays to identify non-host species such as pathogens. Also provided are methods of tracking samples, tracking reagents, and tracking diversity loss in sequencing assays.