公开(公告)号：US12128112B2

公开(公告)日：2024-10-29

申请号：US17272587

申请日：2019-08-14

Applicant: OBSCHESTVO S OGRANICHENNOI OTVETSTVENNOSTJU “PRORYVNYE INNOVATSIONNYE TEKHNOLOGII” ,  CELL AND GENE THERAPY LTD

Inventor： Natalia Savelieva

IPC: A61K48/00 ,  C12N1/20 ,  C12N15/10 ,  C12N15/70 ,  C12N15/85

CPC classification number: A61K48/005 ,  A61K48/0016 ,  C12N1/205 ,  C12N15/1003 ,  C12N15/70 ,  C12N15/85

Abstract: Produced the gene therapy DNA vectors based on the gene therapy DNA vector VTvaf17 for the treatment of diseases featuring disruption of mucociliary transport, mucolytic function and development of mucostasis. The gene therapy DNA vector contains the coding region of the SKI, TGFB3, TIMP2 or FMOD therapeutic genes. Methods of producing or use a gene therapy DNA vector based on gene therapy DNA vector VTvaf17 carrying SKI, TGFB3, TIMP2 or FMOD therapeutic genes. The methods of producing strain for production of gene therapy DNA vector for treatment of diseases featuring disruption of mucociliary transport and development of mucostasis. Escherichia coli strain SCS 110-AF/VTvaf17-SKI, SCS 110-AF/VTvaf17-TGFB3, SCS110-AF/VTvaf17-TIMP2 or SCSI 10-AF/VTvaf17-FMOD obtains by the method described above carrying gene therapy DNA vector VTvaf17-SKI, VTvaf17-TGFB3, VTvaf17-TIMP2 or VTvaf17-FMOD. The method of producing a gene therapy DNA vector carrying SKI, TGFB3, TIMP2 or FMOD therapeutic gene uses on an industrial scale.

公开(公告)号：US20240309427A1

公开(公告)日：2024-09-19

申请号：US18674513

申请日：2024-05-24

Applicant: Karius, Inc.

Inventor： Timothy A. Blauwkamp ,  Fred Christians ,  Michael Rosen ,  Igor D. Vilfan

IPC: C12Q1/6806 ,  C12N15/10 ,  C12Q1/6848 ,  C12Q1/6869 ,  C12Q1/6876

CPC classification number: C12Q1/6806 ,  C12N15/10 ,  C12N15/1003 ,  C12Q1/6848 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2523/313

Abstract: This disclosure provides methods that are useful for reducing or inactivating contaminant nucleic acids.

公开(公告)号：US20240301392A1

公开(公告)日：2024-09-12

申请号：US18655262

申请日：2024-05-05

Applicant: Alex Garvin

Inventor： Alex Garvin

IPC: C12N15/10

CPC classification number: C12N15/1003

Abstract: The present invention provides a method for the isolation of sperm DNA from swabs taken from rape victims without having to perform a change in buffers. Non-sperm cells from the victim are digested with an enzyme and solubilized, and then in the same buffer an enzyme capable of digesting soluble DNA is added and the victim's DNA is degraded, leaving only the rapist's DNA intact. Since no change of buffer is needed, no centrifugation or filtration steps are needed. The inventive method has utility particularly in the forensic science field.

公开(公告)号：US20240218350A1

公开(公告)日：2024-07-04

申请号：US18028746

申请日：2021-09-28

Applicant: TIANJIN SPRING TIDE GENE TECHNOLOGY CO., LTD.

Inventor： Xianglong YANG

IPC: C12N15/10

CPC classification number: C12N15/1003

Abstract: The present invention discloses a method for isolating and purifying solid nucleic acids from biomaterials comprising the steps of (1) mixing the liquid containing naked nucleic acids with a precipitant; centrifuging at room temperature, pouring the supernatant into a new centrifuge tube; (2) adding isopropanol to the new centrifuge tube, mixing well, standing at room temperature, centrifuging, producing a solid, discarding other than the solid; washing, obtaining solid RNAs or solid mixtures of RNAs and DNAs and storing. The invention uses a homogenization-dissociation reagent to homogenize biomaterials, and a liquid containing naked nucleic acids can be easily obtained without heating, which can be used to extract solid RNAs and solid DNAs. By adding a compound that removes secondary metabolites of cells to the homogenization-dissociation reagent, vascular plant RNA, woody plant RNA, and both solid RNAs and solid DNA can be extracted from human blood. The solid RNAs and solid DNAs obtained by the present invention can be stored at room temperature for one month or below −20° C. for one year.

公开(公告)号：US20240180130A1

公开(公告)日：2024-06-06

申请号：US18391184

申请日：2023-12-20

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： John McWhirter ,  Cagan Gurer ,  Karolina A. Meagher ,  Lynn Macdonald ,  Andrew J. Murphy

IPC: A01K67/0278 ,  C07K16/00 ,  C12N15/10

CPC classification number: A01K67/0278 ,  C07K16/00 ,  C12N15/1003 ,  A01K2217/052 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/01 ,  C07K2317/10 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/51 ,  C07K2317/56 ,  C07K2317/64

Abstract: Non-human animals, e.g., mammals, e.g., mice or rats, are provided comprising an immunoglobulin heavy chain locus that comprises a rearranged human immunoglobulin heavy chain variable region nucleotide sequence. The rearranged human immunoglobulin heavy chain variable region nucleotide sequence may be operably linked to a heavy or light chain constant region nucleic acid sequence. Also described are genetically modified non-human animals comprising an immunoglobulin light chain locus comprising one or more but less than the wild type number of human immunoglobulin light chain variable region gene segments, which may be operably linked to a light chain constant region nucleic acid sequence. Also provided are methods for obtaining nucleic acid sequences that encode immunoglobulin light chain variable domains capable of binding an antigen in the absence of a heavy chain.

公开(公告)号：US11981893B2

公开(公告)日：2024-05-14

申请号：US17493637

申请日：2021-10-04

Applicant: Nantomics

Inventor： Patrick Soon-Shiong ,  Shahrooz Rabizadeh ,  Kathleen Danenberg

IPC: C12N15/11 ,  C12N15/10 ,  C12Q1/6876

CPC classification number: C12N15/11 ,  C12N15/1003 ,  C12Q1/6876 ,  C12N2320/51 ,  C12Q2565/00

Abstract: RNA from a biological fluid is stabilized during isolation and/or storage using DNA. In especially preferred aspects, the RNA is cfRNA and/or ctRNA, and the biological fluid is blood.

公开(公告)号：US11976270B2

公开(公告)日：2024-05-07

申请号：US16874480

申请日：2020-05-14

Applicant: Massachusetts Institute of Technology

Inventor： Angela Belcher ,  Christopher A. Voigt ,  Uyanga Tsedev ,  Tae-Gon Cha

IPC: C12N15/70 ,  C12N15/10

CPC classification number: C12N15/1003 ,  C12N15/70 ,  C12N2310/532

Abstract: Provided herein, in some aspects, are methods and compositions for producing single-stranded DNA (ssDNA) having uniform length.

公开(公告)号：US20240094208A1

公开(公告)日：2024-03-21

申请号：US18461820

申请日：2023-09-06

Applicant: Dana-Farber Cancer Institute, Inc.

Inventor： Levi A. Garraway ,  Caroline Emery ,  Nikhil Wagle

IPC: G01N33/574 ,  A61K38/00 ,  A61P35/00 ,  C12N9/12 ,  C12N15/10 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6886

CPC classification number: G01N33/5743 ,  A61K38/005 ,  A61P35/00 ,  C12N9/1205 ,  C12N15/1003 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6886 ,  C12Y207/12002 ,  C12Q2600/106 ,  C12Q2600/136 ,  C12Q2600/156 ,  G01N2333/91205 ,  G01N2500/10 ,  G01N2500/20

Abstract: An isolated nucleic acid molecule encoding a mutant MEK1 protein having MEK1 activity is disclosed. The mutant MEK1 protein comprises an amino acid substitution at position 121 of wild-type MEK1 shown in SEQ ID NO: 2. The amino acid substitution confers resistance to one or more RAF or MEK inhibitors on a cell expressing the mutant MEK1 protein.

公开(公告)号：US20240026339A1

公开(公告)日：2024-01-25

申请号：US18349394

申请日：2023-07-10

Applicant: Vilnius University

Inventor： Linas Mazutis ,  Karolis Goda

IPC: C12N15/10

CPC classification number: C12N15/1003

Abstract: The present invention relates to amphiphiles and specifically the amphiphiles that can be used as cell lysis reagents. The invention reveals the amphiphiles as well as their combinations for efficiently lysing the mammalian cells under relatively mild conditions that are compatible with a reverse transcription and polymerase chain reaction. The invention reveals the use of mixture of amphiphiles to improve the cell lysis and obtain increased yields of copy DNA during the reverse transcription reaction. The invention also provides a method for increasing the diversity of gene and transcript capture during single-cell RNA-sequencing using droplet microfluidics.

公开(公告)号：US20240026335A1

公开(公告)日：2024-01-25

申请号：US17869074

申请日：2022-07-20

Applicant: Imam Abdulrahman Bin Faisal University

Inventor： Khaled Fikry SALAMA ,  Amal Abdullah ALODAINI

IPC: C12N15/10 ,  G01N1/30

CPC classification number: C12N15/1003 ,  G01N1/30 ,  G01N2001/302 ,  G01N2001/305

Abstract: A method of fixating a tissue sample including: treating the tissue sample with a nano-tissue fixative solution to form a fixated tissue sample. A composition of nucleic acids in the fixated tissue sample is not altered. The nano-tissue fixative solution includes acetic acid, at least one alcohol, chloroform, titanium dioxide nanoparticles, zinc oxide nanoparticles, and silver nanoparticles.