公开(公告)号：US11987797B2

公开(公告)日：2024-05-21

申请号：US18057588

申请日：2022-11-21

申请人： LANZHOU VETERINARY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Haixue Zheng ,  Keshan Zhang ,  Ting Zhang ,  Tao Feng ,  Bo Yang ,  Xing Yang ,  Xijuan Shi ,  Hong Tian ,  Yi Ru ,  Fan Yang ,  Zixiang Zhu ,  Jianhong Guo ,  Jijun He ,  Xiangtao Liu

IPC分类号： C12N15/65 ,  C12N7/00 ,  C12N15/63

CPC分类号： C12N15/65 ,  C12N7/00 ,  C12N15/63 ,  C12N2710/12021 ,  C12N2710/12022

摘要： The present disclosure belongs to the technical field of biology, and in particular relates to an attenuated strain of African swine fever virus (ASFV) with IPTG-induced deletion of a D1133L gene and use thereof. In the present disclosure, it is firstly found that the D1133L protein of ASFV can inhibit production of IFN-β and downstream cytokines ISG-15 and ISG-56, and can be used as an immunosuppressant with a relatively strong immunosuppressive effect. In addition, the attenuated strain of ASFV with IPTG-induced deletion of the D1133L gene is constructed. Specifically, a screening expression cassette is inserted into a position before the non-structural protein gene D1133L of the ASFV using an Escherichia coli lac operator-repressor system, to obtain a recombinant virus. In the presence of the IPTG, the recombinant virus has similar characteristics to a wild-type virus; and in the absence of the IPTG, the expression of the D1133L protein is inhibited.

公开(公告)号：US20230365982A1

公开(公告)日：2023-11-16

申请号：US18246564

申请日：2021-09-24

申请人： BONDI BIO PTY LTD

发明人： James BROWN ,  Cameron O'BRIEN ,  Artur WLODARCZYK ,  Dominik KOPP

IPC分类号： C12N15/74 ,  C12N15/11 ,  C12N15/65

CPC分类号： C12N15/74 ,  C12N15/11 ,  C12N15/65

摘要： There is provided nucleic acid constructs comprising two portions of a neutral site and an intervening heterologous nucleic acid comprising landing zones and a selectable marker for producing standardised cyanobacteria cell strains for stain engineering. There is also provided methods for standardised strain engineering using nucleic acid cassettes comprising combinations of landing zones.

公开(公告)号：US20230340499A1

公开(公告)日：2023-10-26

申请号：US18149913

申请日：2023-01-04

申请人： INPAMAC BIOTECH CANADA INC.

发明人： Laszlo Robert KATONA

IPC分类号： C12N15/65 ,  C12N15/85

CPC分类号： C12N15/65 ,  C12N15/85 ,  C12N2800/24 ,  C12N2830/008

摘要： Described are various embodiments of specific sequences for recombinant gene insertion, amplification and expression in the genomes of a variety of mammalian cells are disclosed. A DNA vector containing one or more such sequence enables high levels of recombinant gene expression following transfection, stable integration and amplification. The selection and cloning of the specific loci and the expression of recombinant genes is disclosed, as are the DNA vectors and the host cells.

公开(公告)号：US20230295641A1

公开(公告)日：2023-09-21

申请号：US18057588

申请日：2022-11-21

申请人： LANZHOU VETERINARY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Haixue ZHENG ,  Keshan ZHANG ,  Ting ZHANG ,  Tao FENG ,  Bo YANG ,  Xing YANG ,  Xijuan SHI ,  Hong TIAN ,  Yi RU ,  Fan YANG ,  Zixiang ZHU ,  Jianhong GUO ,  Jijun HE ,  Xiangtao LIU

IPC分类号： C12N15/65 ,  C12N15/63 ,  C12N7/00

CPC分类号： C12N15/65 ,  C12N15/63 ,  C12N7/00 ,  C12N2710/12021 ,  C12N2710/12022

摘要： The present disclosure belongs to the technical field of biology, and in particular relates to an attenuated strain of African swine fever virus (ASFV) with IPTG-induced deletion of a D1133L gene and use thereof. In the present disclosure, it is firstly found that the D1133L protein of ASFV can inhibit production of IFN-β and downstream cytokines ISG-15 and ISG-56, and can be used as an immunosuppressant with a relatively strong immunosuppressive effect. In addition, the attenuated strain of ASFV with IPTG-induced deletion of the D1133L gene is constructed. Specifically, a screening expression cassette is inserted into a position before the non-structural protein gene D1133L of the ASFV using an Escherichia coli lac operator-repressor system, to obtain a recombinant virus. In the presence of the IPTG, the recombinant virus has similar characteristics to a wild-type virus; and in the absence of the IPTG, the expression of the D1133L protein is inhibited.

公开(公告)号：US11624077B2

公开(公告)日：2023-04-11

申请号：US16637591

申请日：2017-08-08

申请人： PEKING UNIVERSITY ,  EDIGENE BIOTECHNOLOGY INC.

发明人： Wensheng Wei ,  Yiou Chen ,  Yuexin Zhou ,  Hongmin Zhang ,  Pengfei Yuan ,  Yuan Liu

IPC分类号： C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N15/65 ,  G01N21/64

摘要： A donor construct and a gene knockout method, as well as a system and kit for the gene knockout are provided. The donor construct is a linear donor DNA or can be cleaved in a cell to produce the linear donor DNA. The gene knockout method uses a marker gene contained in the donor construct to enrich cells in which a gene is knocked out, thereby improving the efficiency of generating the gene knockout by a sequence-specific nuclease.

公开(公告)号：US11530414B2

公开(公告)日：2022-12-20

申请号：US16471872

申请日：2018-10-16

申请人： CELL BIOTECH CO., LTD.

发明人： Myung Jun Chung

IPC分类号： A61K35/747 ,  A61K35/744 ,  A61K35/745 ,  A61K35/00 ,  C12N15/74 ,  C12N15/65 ,  C12R1/23 ,  C12R1/24 ,  C12R1/245

摘要： The present invention relates to a gene expression cassette including a strong promoter derived from lactic acid bacteria, and a gene expression vector including the same. According to the present invention, a large amount of a human protein, the physiological activity of which has been verified, may be stably produced with high efficiency by introducing a useful foreign gene into an expression vector and transforming probiotics with the expression vector. Through the production of this protein, it is possible to provide a basis for developing functional probiotics and making products using them.

公开(公告)号：US11525139B2

公开(公告)日：2022-12-13

申请号：US17378606

申请日：2021-07-16

申请人： Akouos, Inc.

发明人： Emmanuel John Simons ,  Ellen Reisinger ,  Sebastian Kügler ,  Hanan Al-Moyed

IPC分类号： C12N15/52 ,  A01K67/027 ,  A61K38/17 ,  A61K48/00 ,  A61P27/16 ,  C07K14/47 ,  C12N15/65 ,  C12N15/86 ,  C12N15/90 ,  C12N5/0793 ,  C12N9/16

摘要： Provided herein are compositions that include at least two different nucleic acid vectors, where each of the at least two different vectors includes a coding sequence that encodes a different portion of an otoferlin protein, and the use of these compositions to treat hearing loss in a subject.

公开(公告)号：US20220228165A1

公开(公告)日：2022-07-21

申请号：US17610086

申请日：2020-05-11

申请人： CNCURE BIOTECH INC.

发明人： Jung Joon Min ,  Hyon El Choy ,  Yeong Jin Hong ,  Sung Hwan You ,  Mi Ryung Song

IPC分类号： C12N15/85 ,  C12N15/74 ,  C12N15/65 ,  A61P35/00 ,  A61K48/00

摘要： The present disclosure relates to a DNA construct, and a strain into which a recombinant vector comprising the DNA construct has been introduced. The DNA construct according to the present disclosure allows the expression levels of genes, operably linked downstream of first and second promoters, in a host strain or cell, to be balanced, so that cancer diagnosis and treatment may be performed simultaneously. In addition, the DNA construct of the present disclosure completely does not allow the anticancer protein and the reporter protein to be expressed at all in the absence of doxycycline, and thus it allows the anticancer protein to be expressed at an appropriate dose for cancer treatment by controlling whether or not treatment with doxycycline is performed, and at the same time, enables the size of the cancer to be monitored in real time depending on the expression level of the reporter protein.

公开(公告)号：US20220119874A1

公开(公告)日：2022-04-21

申请号：US17480067

申请日：2021-09-20

申请人： Fluidigm Corporation

发明人： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

IPC分类号： C12Q1/6855 ,  C12N15/65 ,  C12N15/66 ,  C12N15/10 ,  C12Q1/686 ,  C12Q1/6874

摘要： Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

公开(公告)号：US11193168B2

公开(公告)日：2021-12-07

申请号：US16789279

申请日：2020-02-12

申请人： EXACT SCIENCES DEVELOPMENT COMPANY, LLC

发明人： Hatim T. Allawi ,  William G. Weisburg ,  Graham P. Lidgard ,  Michael W. Kaiser ,  Abram M. Vaccaro ,  Gracie Shea

IPC分类号： C12Q1/686 ,  C12Q1/6827 ,  C12N15/65 ,  C01B17/48

摘要： Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.