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公开(公告)号：US20240309425A1
公开(公告)日：2024-09-19
申请号：US18370325
申请日：2023-09-19
Applicant: Fluidigm Corporation
Inventor： Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
IPC: C12Q1/6806 , B01L3/00 , B01L7/00 , C12Q1/686
CPC classification number: C12Q1/6806 , B01L3/502738 , C12Q1/686 , B01L3/50273 , B01L7/52 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , B01L2400/0655
Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
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公开(公告)号：US20220413277A1
公开(公告)日：2022-12-29
申请号：US17777894
申请日：2020-11-17
Applicant: Fluidigm Corporation
Inventor： Daaf Sandkuijl , Alan Carpino
Abstract: A microscopy apparatus comprises a microscope comprising a stage configured to hold a tissue sample, a UV laser assembly configured to emit a UV laser beam to a viewing area of the tissue sample, and an IR laser assembly configured to emit an IR laser beam to the viewing area of the tissue sample. The UV and IR laser assemblies are oriented so as to emit the respective UV and IR laser beams in a same direction.
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公开(公告)号：US11078476B2
公开(公告)日：2021-08-03
申请号：US16105711
申请日：2018-08-20
Applicant: Fluidigm Corporation
Inventor： Jian Qin , Peilin Chen , Ramesh Ramakrishnan
IPC: C12N15/10
Abstract: This invention provides a technology for isolating nucleic acids from wax-embedded samples that is superior to the current state of the art. Standard protocols with this objective typically comprise dissolving the wax-embedded sample in an organic solvent, extracting nucleic acids from the organic solvent into an aqueous buffer, and isolating the nucleic acids from the aqueous buffer. The technology described here includes using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.
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公开(公告)号：US10954560B2
公开(公告)日：2021-03-23
申请号：US16215489
申请日：2018-12-10
Applicant: Fluidigm Corporation
Inventor： Carolyn G. Conant , Tze Howe Charn , Jason A. A. West , Xiaohui Wang
IPC: C12Q1/68 , B01L3/00 , C12Q1/6874 , C12Q1/6844
Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.
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公开(公告)号：US10578633B2
公开(公告)日：2020-03-03
申请号：US14775685
申请日：2014-03-14
Applicant: Fluidigm Corporation
Inventor： Jason A. A. West , Brian Fowler
Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment, A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.
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Patent Agency Ranking
公开(公告)号：US20190055540A1
公开(公告)日：2019-02-21
申请号：US16105711
申请日：2018-08-20
Applicant: Fluidigm Corporation
Inventor： Jian Qin , Peilin Chen , Ramesh Ramakrishnan
IPC: C12N15/10
Abstract: This invention provides a technology for isolating nucleic acids from wax-embedded samples that is superior to the current state of the art. Standard protocols with this objective typically comprise dissolving the wax-embedded sample in an organic solvent, extracting nucleic acids from the organic solvent into an aqueous buffer, and isolating the nucleic acids from the aqueous buffer. The technology described here includes using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.
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公开(公告)号：US09840732B2
公开(公告)日：2017-12-12
申请号：US13899397
申请日：2013-05-21
Applicant: Fluidigm Corporation
Inventor： Megan Anderson , Peilin Chen , Brian Fowler , Fiona Kaper , Ronald Lebofsky , Andrew May
CPC classification number: C12Q1/6813 , B01D15/08 , C12Q1/6806 , C12Q2563/159 , C12Q2563/179 , C12Q2565/629
Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out “in parallel,” i.e., essentially simultaneously in the separate reaction volumes.
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公开(公告)号：US20170349934A1
公开(公告)日：2017-12-07
申请号：US15590998
申请日：2017-05-09
Applicant: Fluidigm Corporation
Inventor： Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
CPC classification number: C12Q1/6806 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , B01L2400/0655 , C12Q1/686 , C12Q2525/155 , C12Q2525/161 , C12Q2527/143 , C12Q2535/122 , C12Q2549/119 , C12Q2563/179 , C12Q2565/629
Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
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公开(公告)号：US20170321249A1
公开(公告)日：2017-11-09
申请号：US15495695
申请日：2017-04-24
Applicant: Fluidigm Corporation
Inventor： Marc A. Unger , Geoffrey Richard Facer , Barry Clerkson , Christopher G. Cesar , Neil Switz
IPC: C12Q1/68 , G02B21/16 , G01N21/64 , G01N21/75 , G01N27/447 , G01N21/84 , G02B21/36 , B01L3/00 , B01L7/00
CPC classification number: C12Q1/686 , B01J2219/00576 , B01J2219/00704 , B01L3/5027 , B01L7/52 , G01N21/64 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/75 , G01N21/8483 , G01N27/44721 , G01N2021/6421 , G01N2201/061 , G02B21/16 , G02B21/36
Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
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公开(公告)号：US09663821B2
公开(公告)日：2017-05-30
申请号：US14960754
申请日：2015-12-07
Applicant: Fluidigm Corporation
Inventor： Marc A. Unger , Geoffrey Richard Facer , Barry Clerkson , Christopher G. Cesar , Neil Switz
IPC: G01N21/00 , G01N15/06 , C12Q1/68 , G01N21/64 , G01N27/447 , G02B21/16 , G01N21/84 , G01N21/75 , G02B21/36 , B01L3/00 , B01L7/00
CPC classification number: C12Q1/686 , B01J2219/00576 , B01J2219/00704 , B01L3/5027 , B01L7/52 , G01N21/64 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/75 , G01N21/8483 , G01N27/44721 , G01N2021/6421 , G01N2201/061 , G02B21/16 , G02B21/36
Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
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