公开(公告)号：US12129475B2

公开(公告)日：2024-10-29

申请号：US16484200

申请日：2018-02-08

申请人： YEDA RESEARCH AND DEVELOPMENT CO. LTD. ,  Yissum Research Development Company of The Hebrew University of Jerusalem Ltd.

发明人： Asaph Aharoni ,  Prashant Sonawane ,  Pablo D. Cardenas ,  Burdman Saul

IPC分类号： C12N15/82 ,  C12N9/02 ,  C12N9/04

CPC分类号： C12N15/8279 ,  C12N9/0006 ,  C12N9/0071 ,  C12N15/8218 ,  C12N15/8243

摘要： Disclosed herein are genetically modified plants having altered biological activity of 3-β-hydroxysteroid dehydrogenase/isomerase (GAME25), or 2-oxoglutarate-dependent dioxygenase (GAME31), or a combination thereof, wherein the genetically modified plants have an altered content of at least one cholesterol derived compound selected from the group including a steroidal alkaloid or a glycosylated derivative thereof and an unsaturated or saturated steroidal saponin or a glycoside derivative thereof. Further disclosed herein are genetically modified plants having altered expression of a gene encoding a 3-β-hydroxysteroid dehydrogenase/isomerase (GAME25), or a 2-oxoglutarate-dependent dioxygenase (GAME31), or a combination thereof, wherein the genetically modified plant has an altered content of at least one cholesterol derived compound selected from the group including a steroidal alkaloid or a glycosylated derivative thereof and an unsaturated or saturated steroidal saponin or a glycoside derivative thereof. Methods of producing these genetically modified plants are also disclosed.

公开(公告)号：US20240315192A1

公开(公告)日：2024-09-26

申请号：US18735258

申请日：2024-06-06

申请人： YEDA RESEARCH AND DEVELOPMENT CO., LTD.

发明人： Asaph AHARONI ,  Prashant SONAWANE ,  Maxim ITKIN ,  Adam JOZWIAK

IPC分类号： A01H6/82 ,  C12N9/10 ,  C12N15/82

CPC分类号： A01H6/82 ,  C12N9/1059 ,  C12N15/8218 ,  C12N15/8243 ,  C12N15/8247

摘要： Provided herein are genetically modified cells and genetically modified plants having increased or decreased expression of a cellulose synthase like G (CSLG) enzyme. These cells and plants may have an increased or decreased content a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin, compared to a corresponding unmodified cell or plant. Also provided herein are methods of producing a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a genetically modified cell, as well as methods of reducing the content of a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a cell of a plant or a plant part, and methods of increasing the content of a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a cell of a plant or a plant part.

公开(公告)号：US12077763B2

公开(公告)日：2024-09-03

申请号：US17689598

申请日：2022-03-08

申请人： Greenlight Biosciences, Inc. ,  United States of America, as represented by the Secretary of Agriculture

发明人： Adriana Puralewski ,  Christopher Lawrence ,  Upendra Kumar Devisetty ,  Sambit Kumar Mishra ,  Krishnakumar Sridharan ,  Lance E. Cadle Davidson ,  Anna N. Underhill ,  Yufeng Fang ,  Wimalanathan Kokulapalan

IPC分类号： C12N15/82 ,  C12N15/113

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8286

摘要： Disclosed herein are compositions and methods that involve recombinant polynucleotide molecules, such as single or double-stranded DNA or RNA molecules, also referred to “triggers”, that are useful for controlling or preventing E. necator infection, or recombinant DNA constructs for making such RNA molecules or for making transgenic grape plants resistant to E. necator infection. In some embodiments, polynucleotide triggers are provided as topically applied agents for controlling or preventing infection of a plant by E. necator.

公开(公告)号：US20240279675A1

公开(公告)日：2024-08-22

申请号：US17775822

申请日：2020-11-12

申请人： University of Maryland, College Park ,  The Regents of the University of California

发明人： Anne Elizabeth Simon ,  Jingyuan Liu ,  Georgios Vidalakis ,  Sohrab Bodaghi

IPC分类号： C12N15/82

CPC分类号： C12N15/8281 ,  C12N15/8218

摘要： The present disclosure relates to a single stranded RNA vector suitable for introducing a therapeutic agent, such as a peptide, a protein or a small RNA, into a host plant. The vector does not encode for any movement protein or coat protein, but is capable of systemic and phloem-limited movement and replication within the host plant.

公开(公告)号：US20240263253A1

公开(公告)日：2024-08-08

申请号：US18256472

申请日：2023-04-20

申请人： Nantong University

发明人： Baohua Wang ,  Kangtai Sun ,  Zhimin Zhuang ,  Hui Fang ,  Kai Wang ,  Jinlei Han ,  Meijun Ji ,  Wenxiang Feng

IPC分类号： C12Q1/6895 ,  C12N15/10 ,  C12N15/82 ,  C12Q1/6851

CPC分类号： C12Q1/6895 ,  C12N15/1096 ,  C12N15/8218 ,  C12N15/8273 ,  C12Q1/6851 ,  C12Q2600/13

摘要： The present disclosure relates to the technical field of plant genetics, particularly to a discovery method of cotton salt-tolerant genes based on combined transcriptome and proteome analysis and use thereof. In the present disclosure, upland cotton salt-tolerant variety Tongyan No. 1 serving as a material is subjected to transcriptome and proteome sequencing under salt stress and control; salt-tolerant candidate genes are preliminarily determined via the combined analysis and salt-tolerant differential genes are subjected to reverse transcription-quantitative polymerase chain reaction (RT-qPCR) expression analysis, and finally function validation is conducted in cotton. The two cotton salt-tolerate genes Gh_D10G0907 and Gh_D11G0978 obtained in the present disclosure are respectively located on cotton chromosomes D10 and D11; the molecular marker-assisted breeding of salt-tolerate cotton varieties can be achieved by utilizing the two genes.

公开(公告)号：US20240263187A1

公开(公告)日：2024-08-08

申请号：US18594315

申请日：2024-03-04

申请人： UNIVERSITY OF NORTH TEXAS

发明人： Richard A. Dixon ,  Fang Chen ,  Chunliu Zhuo

IPC分类号： C12N15/82

CPC分类号： C12N15/8255 ,  C12N15/8218

摘要： The disclosure provides recombinant LAC polynucleotides encoding a laccase (LAC) polypeptide capable of polymerizing caffeyl alcohol, vectors and cells including the recombinant LAC polynucleotide. The disclosure also provides transgenic plants including cells having the recombinant LAC polynucleotide of the present disclosure and methods of increasing production of C-lignin in a plant.

公开(公告)号：US20240247277A1

公开(公告)日：2024-07-25

申请号：US18630328

申请日：2024-04-09

申请人： Altria Client Services LLC

发明人： Chengalrayan KUDITHIPUDI ,  Alec J. HAYES

IPC分类号： C12N15/82 ,  A01H1/06 ,  A24B13/00

CPC分类号： C12N15/8243 ,  A01H1/06 ,  A24B13/00 ,  C12N15/8218

摘要： This disclosure provides tobacco plants having a mutation in PR50 and transgenic tobacco plants containing a PR50 RNAi, and methods of making and using such plants.

公开(公告)号：US12041907B2

公开(公告)日：2024-07-23

申请号：US17272685

申请日：2019-09-05

申请人： YEDA RESEARCH AND DEVELOPMENT CO. LTD.

发明人： Asaph Aharoni ,  Prashant Sonawane ,  Maxim Itkin ,  Adam Jozwiak

IPC分类号： A01H6/82 ,  C12N9/10 ,  C12N15/82

CPC分类号： A01H6/82 ,  C12N9/1059 ,  C12N15/8218 ,  C12N15/8243 ,  C12N15/8247

摘要： Provided herein are genetically modified cells and genetically modified plants having increased or decreased expression of a cellulose synthase like G (CSLG) enzyme. These cells and plants may have an increased or decreased content a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin, compared to a corresponding unmodified cell or plant. Also provided herein are methods of producing a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a genetically modified cell, as well as methods of reducing the content of a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a cell of a plant or a plant part, and methods of increasing the content of a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a cell of a plant or a plant part.

公开(公告)号：US12035666B2

公开(公告)日：2024-07-16

申请号：US17691549

申请日：2022-03-10

申请人： Syngenta Participations AG

发明人： Timothy Kelliher ,  Satya Chintamanani ,  Brent Delzer ,  Michael L Nuccio ,  Robert Arthur Dietrich ,  Suresh Babu Kadaru ,  Todd Lee Warner ,  William Paul Bullock

IPC分类号： A01H1/08 ,  A01H1/04 ,  C12N9/18 ,  C12N15/82 ,  C12Q1/6895

CPC分类号： A01H1/08 ,  A01H1/045 ,  C12N9/18 ,  C12N15/8218 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8287 ,  C12Q1/6895 ,  C12Y301/01002 ,  C12N2310/14 ,  C12N2310/20 ,  Y02A40/146

摘要： Provided here are methods of using a mutated patatin-like phospholipase IIα (“pPLAIIα,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAIIα to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAIIα. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

公开(公告)号：US20240099213A1

公开(公告)日：2024-03-28

申请号：US18524717

申请日：2023-11-30

申请人： Syngenta Participations AG

发明人： Timothy Kelliher ,  Satya Chintamanani ,  Brent Delzer ,  Michael L Nuccio ,  Robert Arthur Dietrich ,  Suresh Babu Kadaru ,  Todd Lee Warner ,  William Paul Bullock

IPC分类号： A01H1/08 ,  A01H1/04 ,  C12N9/18 ,  C12N15/82 ,  C12Q1/6895

CPC分类号： A01H1/08 ,  A01H1/045 ,  C12N9/18 ,  C12N15/8218 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8287 ,  C12Q1/6895 ,  C12Y301/01002 ,  C12N2310/14 ,  C12N2310/20 ,  Y02A40/146

摘要： Provided here are methods of using a mutated patatin-like phospholipase IIα (“pPLAIIα,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAIIα to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAIIα. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.