公开(公告)号：US20240263253A1

公开(公告)日：2024-08-08

申请号：US18256472

申请日：2023-04-20

申请人： Nantong University

发明人： Baohua Wang ,  Kangtai Sun ,  Zhimin Zhuang ,  Hui Fang ,  Kai Wang ,  Jinlei Han ,  Meijun Ji ,  Wenxiang Feng

IPC分类号： C12Q1/6895 ,  C12N15/10 ,  C12N15/82 ,  C12Q1/6851

CPC分类号： C12Q1/6895 ,  C12N15/1096 ,  C12N15/8218 ,  C12N15/8273 ,  C12Q1/6851 ,  C12Q2600/13

摘要： The present disclosure relates to the technical field of plant genetics, particularly to a discovery method of cotton salt-tolerant genes based on combined transcriptome and proteome analysis and use thereof. In the present disclosure, upland cotton salt-tolerant variety Tongyan No. 1 serving as a material is subjected to transcriptome and proteome sequencing under salt stress and control; salt-tolerant candidate genes are preliminarily determined via the combined analysis and salt-tolerant differential genes are subjected to reverse transcription-quantitative polymerase chain reaction (RT-qPCR) expression analysis, and finally function validation is conducted in cotton. The two cotton salt-tolerate genes Gh_D10G0907 and Gh_D11G0978 obtained in the present disclosure are respectively located on cotton chromosomes D10 and D11; the molecular marker-assisted breeding of salt-tolerate cotton varieties can be achieved by utilizing the two genes.