公开(公告)号：US12110494B2

公开(公告)日：2024-10-08

申请号：US17273621

申请日：2019-09-05

Applicant: Korea University Research and Business Foundation

Inventor： Kyoung Heon Kim ,  Yong Su Jin ,  Dong-Hyun Kim ,  Eun-Ju Yun

IPC: C12N15/81 ,  C07K14/195 ,  C12N9/24 ,  C12N9/38 ,  C12P7/18

CPC classification number: C12N15/81 ,  C07K14/195 ,  C12N9/2471 ,  C12P7/18 ,  C12Y101/01021 ,  C12Y302/01023

Abstract: The present invention relates to a method for biologically producing, from seaweeds, 3,6-anhydro-L-galactitol (L-AHGol) which is a novel sugar alcohol, and agarobititol (ABol) which is in a disaccharide form having 3,6-anhydro-L-galactitol (L-AHGol) as a reducing end, by using a genetic engineering technique in GRAS strains.

公开(公告)号：US20240294892A1

公开(公告)日：2024-09-05

申请号：US18007162

申请日：2020-07-28

Applicant: QUANTUM HI-TECH (GUANGDONG) BIOLOGICAL CO., LTD.

Inventor： Yuan'an Wei ,  Xianwei Zeng ,  Zhilong Xie ,  Xinqiu Yang ,  Zijian Chen ,  Jingang Huan

IPC: C12N9/38 ,  C12N1/20 ,  C12P21/02 ,  C12R1/09

CPC classification number: C12N9/2471 ,  C12N1/205 ,  C12P21/02 ,  C12R2001/09 ,  C12Y302/01023

Abstract: The present disclosure belongs to the technical field of biochemical engineering, and relates to a preparation method of β-galactosidase. The preparation method includes the following steps: step 1, seeding Bacillus circulans into a Luria-Bertani (LB) medium for culture and activation and then into a seed tank for culture to obtain a seed culture broth; step 2, introducing the seed culture broth into a fermentor containing a fermentation medium for fermentation to obtain a fermentation broth of B. circulans; and step 3, filtering or centrifuging the fermentation broth of B. circulans to remove cells to obtain a β-galactosidase liquid; the fermentation medium includes lactose, galactose, phytone, corn meal, yeast extract, a phosphate salt, a carbonate salt, and water. According to the method, fermentation time is short, production period is shortened, and specific activity of the resulting enzyme liquid is high. The present disclosure further provides a preparation method of an immobilized β-galactosidase without using a crosslinking agent. The resulting immobilized enzyme has excellent stability and can be continuously used for 264 hours or more, and costs for preparing galactose by using the immobilized enzyme is substantially reduced.

公开(公告)号：US11999675B2

公开(公告)日：2024-06-04

申请号：US16983121

申请日：2020-08-03

Applicant: Translate Bio, Inc.

Inventor： Frank DeRosa ,  Braydon Charles Guild ,  Michael Heartlein

IPC: A61K47/00 ,  A61K9/127 ,  A61K9/50 ,  A61K38/18 ,  A61K38/47 ,  A61K48/00 ,  C07C211/21 ,  C12N15/85

CPC classification number: C07C211/21 ,  A61K9/1271 ,  A61K9/1272 ,  A61K9/5015 ,  A61K38/1816 ,  A61K38/47 ,  C12N15/85 ,  C12Y302/01023

Abstract: Disclosed herein are novel compounds, pharmaceutical compositions comprising such compounds and related methods of their use. The compounds described herein are useful, e.g., as liposomal delivery vehicles to facilitate the delivery of encapsulated polynucleotides to target cells and subsequent transfection of said target cells, and in certain embodiments are characterized as having one or more properties that afford such compounds advantages relative to other similarly classified lipids.

公开(公告)号：US11981947B2

公开(公告)日：2024-05-14

申请号：US17814333

申请日：2022-07-22

Applicant: Chr. Hansen HMO GmbH

Inventor： Stefan Jennewein

IPC: C12P19/12 ,  C07H1/00 ,  C07H1/06 ,  C12N1/20 ,  C12N15/72 ,  C12P19/00 ,  C12P19/14 ,  C12P19/26 ,  C12R1/19

CPC classification number: C12P19/26 ,  C07H1/00 ,  C07H1/06 ,  C12N1/205 ,  C12N15/72 ,  C12P19/00 ,  C12P19/12 ,  C12P19/14 ,  C12Y302/01 ,  C12Y302/01023 ,  C12N2800/101 ,  C12R2001/19

Abstract: The present invention relates to the use of one or more glycosidases in the process for the production and/or purification of a produced desired oligosaccharide. The process is preferably a microbial fermentation process using a host microorganism, which may also comprise nucleic acids expressing sugar catabolic pathway proteins suitable for the degradation of saccharides otherwise hindering the purification of the desired oligosaccharide.

公开(公告)号：US20240130949A1

公开(公告)日：2024-04-25

申请号：US18545611

申请日：2023-12-19

Applicant: Dennis C. Mynarcik

Inventor： Dennis C. Mynarcik

IPC: A61K8/66 ,  A61Q11/00 ,  C12N9/22 ,  C12N9/38 ,  C12N9/76

CPC classification number: A61K8/66 ,  A61Q11/00 ,  C12N9/22 ,  C12N9/2471 ,  C12N9/6427 ,  C12Y302/01023 ,  C12Y304/21001 ,  C12Y304/21004 ,  C12Y304/21064

Abstract: Disclosed are compositions and formulations comprising enzymes or other biocatalyst that cleave surface-accessible DNA polymers and/or glycoprotein carbohydrate chains at galactose residues in dental calculus, and optionally further include one or more proteolytic enzymes, thereby destroying the structural integrity of the calculus, and allowing it to be readily removed without requiring special treatment by a trained dental professional. Also disclosed are methods for removing dental calculus using the disclosed compositions and formulations.

公开(公告)号：US11896024B2

公开(公告)日：2024-02-13

申请号：US17687786

申请日：2022-03-07

Applicant: Novozymes A/S

Inventor： Jeppe Wegener Tams

IPC: A23C9/12 ,  A23C9/13 ,  A23C9/15 ,  C12P19/18 ,  C12P19/04

CPC classification number: A23C9/1206 ,  A23C9/1307 ,  A23C9/1512 ,  C12P19/04 ,  C12P19/18 ,  C12Y302/01023 ,  C12Y302/01108

Abstract: The present invention relates to compositions, particularly liquid compositions, comprising enzymes, methods of making the compositions, and uses of the same for making, e.g., dairy products.

公开(公告)号：US20230225346A1

公开(公告)日：2023-07-20

申请号：US18149923

申请日：2023-01-04

Applicant: DSM IP ASSETS B.V.

Inventor： Petrus Jacobus Theodorus DEKKER ,  Luppo EDENS ,  Maximiliaan Peter Marie DE SWAAF ,  Albertus Alard VAN DIJK

IPC: A23C9/127 ,  C12N9/16 ,  C12N9/38 ,  A23C9/12 ,  C12N9/62 ,  A23C19/032 ,  C12N9/24

CPC classification number: A23C9/127 ,  C12N9/16 ,  C12N9/2471 ,  C12Y301/06001 ,  A23C9/1206 ,  C12N9/62 ,  C12Y302/01023 ,  C12N9/2468 ,  A23C19/0328 ,  C12N9/2402 ,  C12Y302/01108

Abstract: The present invention describes a intracellular produced lactase, which comprises less than 40 units arylsulfatase activity per NLU of lactase activity. The invention also provides a process comprising treating a substrate with an enzyme preparation, wherein the enzyme preparation is substantially free from arylsulfatase.

公开(公告)号：US20230225345A1

公开(公告)日：2023-07-20

申请号：US18151319

申请日：2023-01-06

Applicant: Novozymes A/S

Inventor： Cedric Hobel ,  Karen Jensen

IPC: A23C9/12 ,  C12N9/38 ,  A23C9/13

CPC classification number: A23C9/1206 ,  C12N9/2471 ,  A23C9/1322 ,  C12Y302/01023 ,  C12Y302/01108

Abstract: The present invention relates to a sterile-filtered liquid lactase preparation and to a method of sterile filtering a liquid lactase preparation.

公开(公告)号：US20180251771A1

公开(公告)日：2018-09-06

申请号：US15980349

申请日：2018-05-15

Applicant: Glycosyn LLC

Inventor： Massimo Merighi ,  John M. McCoy ,  Matthew Ian Heidtman

IPC: C12N15/70 ,  C12P19/26 ,  C12P19/00 ,  C07H13/04 ,  C12N9/00 ,  C12P19/18 ,  C12N9/38 ,  C12N9/10 ,  C07H3/06

CPC classification number: C12P19/00 ,  C07H3/06 ,  C07H13/04 ,  C12N9/00 ,  C12N9/1051 ,  C12N9/14 ,  C12N9/2471 ,  C12N15/70 ,  C12P19/18 ,  C12P19/26 ,  C12Y204/01 ,  C12Y204/01065 ,  C12Y302/01023 ,  C12Y306/03018 ,  Y02P20/52

Abstract: The invention provides compositions and methods for engineering bacteria to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.

公开(公告)号：US09977016B2

公开(公告)日：2018-05-22

申请号：US15344150

申请日：2016-11-04

Applicant: Kansas State University Research Foundation

Inventor： Robert DeLong ,  Miranda Hurst

IPC: G01N33/543 ,  G01N21/64 ,  C12Q1/68

CPC classification number: G01N33/54346 ,  C12Q1/68 ,  C12Y113/12007 ,  C12Y302/01023 ,  G01N21/6428 ,  G01N2021/6419 ,  G01N2021/6421 ,  C12Q2563/107 ,  C12Q2563/155

Abstract: Methods of characterizing nanoparticles, nanoparticle complexes, and their biomolecular interactions are provided. Concurrent excitation and emission wavelength scans are performed and the optimal fluorescence intensity for the intersect of these wavelengths is determined. The intersect is dependent upon the physical characteristics of the particle and can be used to verify, for example, attachment of biomolecules, amount of biomolecules present, and structure of the attached biomolecule.