公开(公告)号：US12031122B2

公开(公告)日：2024-07-09

申请号：US18319442

申请日：2023-05-17

Applicant: GENECIS BIOINDUSTRIES INC.

Inventor： Adam William Westbrook

IPC: C12N1/20 ,  C07K14/21 ,  C12N1/21 ,  C12N9/04 ,  C12N9/10 ,  C12N9/90 ,  C12N15/52 ,  C12P7/52 ,  C12P7/625 ,  C12R1/19

CPC classification number: C12N1/20 ,  C07K14/21 ,  C12N1/205 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/90 ,  C12N15/52 ,  C12P7/52 ,  C12P7/625 ,  C12Y101/01036 ,  C12Y203/01009 ,  C12Y203/01016 ,  C12Y208/03001 ,  C12Y504/99002 ,  C12R2001/19

Abstract: The disclosure provides recombinant bacterial host cells that metabolize and convert glycerol or volatile fatty acids (VFAs) to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) or PHBV. The disclosure further provides methods of producing PHBV using the recombinant bacteria disclosed herein.

公开(公告)号：US12031123B2

公开(公告)日：2024-07-09

申请号：US18319444

申请日：2023-05-17

Applicant: GENECIS BIOINDUSTRIES INC.

Inventor： Adam William Westbrook

IPC: C12P7/625 ,  C07K14/21 ,  C12N1/20 ,  C12N1/21 ,  C12N9/04 ,  C12N9/10 ,  C12N9/90 ,  C12N15/52 ,  C12P7/52 ,  C12R1/19

CPC classification number: C12N1/20 ,  C07K14/21 ,  C12N1/205 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/90 ,  C12N15/52 ,  C12P7/52 ,  C12P7/625 ,  C12Y101/01036 ,  C12Y203/01009 ,  C12Y203/01016 ,  C12Y208/03001 ,  C12Y504/99002 ,  C12R2001/19

Abstract: The disclosure provides recombinant bacterial host cells that metabolize and convert glycerol or volatile fatty acids (VFAs) to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) or PHBV. The disclosure further provides methods of producing PHBV using the recombinant bacteria disclosed herein.

公开(公告)号：US20230374557A1

公开(公告)日：2023-11-23

申请号：US18319444

申请日：2023-05-17

Applicant: GENECIS BIOINDUSTRIES INC.

Inventor： Adam William WESTBROOK

IPC: C12P7/625 ,  C12N15/52 ,  C12N9/10 ,  C12N9/90 ,  C12N9/04 ,  C07K14/21 ,  C12N1/20

CPC classification number: C12P7/625 ,  C12N15/52 ,  C12N9/13 ,  C12Y208/03001 ,  C12N9/90 ,  C12Y504/99002 ,  C12N9/1029 ,  C12Y203/01009 ,  C12N9/0006 ,  C12Y101/01036 ,  C12Y203/01016 ,  C07K14/21 ,  C12N1/20

Abstract: The disclosure provides recombinant bacterial host cells that metabolize and convert glycerol or volatile fatty acids (VFAs) to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) or PHBV. The disclosure further provides methods of producing PHBV using the recombinant bacteria disclosed herein.

公开(公告)号：US11549129B2

公开(公告)日：2023-01-10

申请号：US16230660

申请日：2018-12-21

Applicant: GENECIS BIOINDUSTRIES INC.

Inventor： Luna Yu ,  Hasitha de Alwis Weerasekera ,  Marcos Forattini Lemos Igreja ,  Vani Sankar ,  Michael James Williamson ,  Sudhanshu Sanjay Soman ,  Kaitlyn Chow

IPC: C12P7/62 ,  C12M1/00 ,  C12M1/26 ,  C12P7/625 ,  C12M1/33

Abstract: According to one broad aspect of this disclosure, a method is provided for producing polyhydroxyalkanoates (PHA) from organic waste. The method comprises homogenizing organic waste to obtain a feedstock that has 1:1 to 3:1 (w/w) water to organic waste ratio. The feedstock is inoculated with an inoculum of acidogenic fermentative bacteria in order to obtain an inoculated feedstock. The inoculated feedstock is incubated for 5 to 10 days, 3 to 10 days, optionally 7 days, optionally 3 days, to obtain a fermentation broth. The fermentation broth comprises volatile fatty acids (VFAs) and undigested organic waste. The fermentation broth is filtered with a filter with a pore size ranging from 0.2 μm to 500,000 NMWC to remove the acidogenic fermentative bacteria and undigested organic waste, to obtain a clarified broth comprising concentrated VFAs. The clarified broth and high-PHA producing bacteria are incubated to produce intracellular PHA granules in the high-PHA producing bacteria. PHA polymers are extracted from the intracellular PHA granules.

公开(公告)号：US20230374445A1

公开(公告)日：2023-11-23

申请号：US18319442

申请日：2023-05-17

Applicant: GENECIS BIOINDUSTRIES INC.

Inventor： Adam William WESTBROOK

IPC: C12N1/20 ,  C12P7/52

CPC classification number: C12N1/205 ,  C12P7/52 ,  C12R2001/19

Abstract: The disclosure provides recombinant bacterial host cells that metabolize and convert glycerol or volatile fatty acids (VFAs) to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) or PHBV. The disclosure further provides methods of producing PHBV using the recombinant bacteria disclosed herein.

公开(公告)号：US20190203237A1

公开(公告)日：2019-07-04

申请号：US16230660

申请日：2018-12-21

Applicant: GENECIS BIOINDUSTRIES INC.

Inventor： Luna Yu ,  Hasitha de Alwis Weerasekera ,  Marcos Forattini Lemos Igreja ,  Vani Sankar ,  Michael James Williamson ,  Sudhanshu Sanjay Soman ,  Kaitlyn Chow

IPC: C12P7/62 ,  C12M1/26 ,  C12M1/00

CPC classification number: C12P7/62 ,  C12M29/00 ,  C12M33/14

Abstract: According to one broad aspect of this disclosure, a method is provided for producing polyhydroxyalkanoates (PHA) from organic waste. The method comprises homogenizing organic waste to obtain a feedstock that has 1:1 to 3:1 (w/w) water to organic waste ratio. The feedstock is inoculated with an inoculum of acidogenic fermentative bacteria in order to obtain an inoculated feedstock. The inoculated feedstock is incubated for 5 to 10 days, 3 to 10 days, optionally 7 days, optionally 3 days, to obtain a fermentation broth. The fermentation broth comprises volatile fatty acids (VFAs) and undigested organic waste. The fermentation broth is filtered with a filter with a pore size ranging from 0.2 μm to 500,000 NMWC to remove the acidogenic fermentative bacteria and undigested organic waste, to obtain a clarified broth comprising concentrated VFAs. The clarified broth and high-PHA producing bacteria are incubated to produce intracellular PHA granules in the high-PHA producing bacteria. PHA polymers are extracted from the intracellular PHA granules.