公开(公告)号：US11732279B2

公开(公告)日：2023-08-22

申请号：US17744782

申请日：2022-05-16

申请人： Nanjing Haohe Biotechnology Co., Ltd.

发明人： Hao Liu ,  Qing Xu

IPC分类号： C12P7/46 ,  C12N1/14 ,  C12N15/80 ,  C12R1/685

CPC分类号： C12P7/46 ,  C12N1/145 ,  C12N15/80 ,  C12N2800/10 ,  C12R2001/685

摘要： The disclosure discloses an Aspergillus niger engineered strain for reducing byproduct fumaric acid in a fermentation process of L-malic acid. The Aspergillus niger engineered strain is an Aspergillus niger engineered strain in which a fumarate hydratase gene fum is knocked out. The disclosure overcomes the defects in the prior art, in the current process of producing malic acid through fermentation of Aspergillus niger, byproduct fumaric acid can be accumulated with the generation of malic acid so as to cause the improved cost of the subsequent malic acid purification process. The disclosure provides an Aspergillus niger engineered strain in which a fum gene is knocked out and a method for greatly reducing byproduct fumaric acid in the fermentation production of Aspergillus niger.

公开(公告)号：US20240174722A1

公开(公告)日：2024-05-30

申请号：US18281117

申请日：2022-03-11

申请人： TENZA, INC.

发明人： Anik Debnath ,  Ameet Shetty

IPC分类号： C07K14/395 ,  A61K36/064 ,  A61K38/00 ,  C12N1/18 ,  C12R1/685 ,  C12R1/84 ,  C12R1/87 ,  C12R1/885

CPC分类号： C07K14/395 ,  A61K36/064 ,  C12N1/185 ,  A61K38/00 ,  C07K2319/02 ,  C12R2001/685 ,  C12R2001/84 ,  C12R2001/87 ,  C12R2001/885

摘要： Provided herein are signal peptides that direct secretion of expressed payload proteins in yeast. Methods of using the signal peptides for therapeutic and non-therapeutic utilities are also provided. Compositions comprising yeast comprising the signal peptides and methods of using said yeast comprising the signal peptides for therapeutic and non-therapeutic utilities are also provided. Methods to design and generate the disclosed signal peptides are also provided.

公开(公告)号：US20240271175A1

公开(公告)日：2024-08-15

申请号：US18251533

申请日：2021-11-02

申请人： Novozymes A/S

发明人： Aki Tomiki-Hashizume ,  Hiroshi Teramoto

IPC分类号： C12P21/02 ,  C07K7/06 ,  C12N1/14 ,  C12N9/34 ,  C12N15/62 ,  C12N15/80 ,  C12R1/685

CPC分类号： C12P21/02 ,  C07K7/06 ,  C12N1/14 ,  C12N9/2428 ,  C12N15/625 ,  C12N15/80 ,  C07K2319/02 ,  C12R2001/685

摘要： The present invention relates to leader peptides, leader peptide fusion proteins, signal peptides, polynucleotides encoding the leader peptides and signal peptides, and to nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing a polypeptide of interest in host cells expressing the leader peptides in translational fusion with the polypeptide of interest.

公开(公告)号：US11718854B2

公开(公告)日：2023-08-08

申请号：US17161502

申请日：2021-01-28

申请人： Battelle Memorial Institute ,  National Technology & Engineering Solutions of Sandia, LLC

发明人： Scott E. Baker ,  Jon K. Magnuson ,  Morgann C. Reilly ,  Joonhoon Kim ,  John Gladden ,  Jed J. Lynn

IPC分类号： C12N1/15 ,  C12N9/34 ,  C12N15/80 ,  C12N15/52 ,  C12P21/00 ,  C12P19/14 ,  C12N9/42 ,  C12N9/20 ,  C12N9/50 ,  C07K14/38 ,  C12N9/24 ,  C12P21/02 ,  C07K14/37 ,  C12N1/14 ,  C12R1/685

CPC分类号： C12N15/52 ,  C07K14/37 ,  C07K14/38 ,  C12N1/145 ,  C12N9/20 ,  C12N9/2402 ,  C12N9/2428 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/50 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/0102 ,  C12R2001/685 ,  C12Y301/01001 ,  C12Y301/01003 ,  C12Y302/01003 ,  C12Y302/01021 ,  C12Y302/01176 ,  C12Y304/21

摘要： Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.

公开(公告)号：US11981888B2

公开(公告)日：2024-05-14

申请号：US17527142

申请日：2021-11-15

申请人： BP Corporation North America Inc.

发明人： Joseph B. Binder

IPC分类号： C12N1/14 ,  C07D307/44 ,  C07D307/48 ,  C07D307/56 ,  C07H19/01 ,  C12N9/24 ,  C12R1/685

CPC分类号： C12N1/145 ,  C07D307/44 ,  C07D307/48 ,  C07D307/56 ,  C07H19/01 ,  C12N9/24 ,  C12R2001/685

摘要： The present invention provides a method of preparing a furan derivative comprising the steps of (a) converting a monosaccharide to provide a keto-intermediate product; and (b) dehydrating the keto-intermediate product to provide a furan derivative; wherein the keto-intermediate product is pre-disposed to forming keto-furanose tautomers in solution. The method may further comprise a step of oxidizing the furan derivative to provide a furandicarboxylic acid or a furandicarboxylic acid derivative.

公开(公告)号：US20230241147A1

公开(公告)日：2023-08-03

申请号：US18063846

申请日：2022-12-09

申请人： NATURE BIO PHARMA CO

发明人： Seung Jin LEE ,  Joong Hyeok LEE

IPC分类号： A61K36/258 ,  C12N1/14 ,  C12P33/00 ,  A61P31/14 ,  A61K31/704

CPC分类号： A61K36/258 ,  C12N1/14 ,  C12P33/00 ,  A61P31/14 ,  A61K31/704 ,  C12R2001/685

摘要： The present invention relates to a method for improving the preparation of fermented black color ginseng containing functional ingredients at high contents based on safety, specifically to a novel Aspergillus niger KHNT-1 strain deposited with accession number KCTC14739BP; an improved process for preparing fermented black color ginseng through treatment with the strain, steaming utilizing pottery, and drying utilizing an LED dryer; fermented black color ginseng containing functional ingredients, ginsenosides Rg5, Rg3, and Rk1, and CK at high contents; and a composition for preventing, improving, or treating coronavirus infectious disease, the composition containing the fermented black color ginseng as an active ingredient.
As the fermented black color ginseng is prepared through fermentation using the novel Aspergillus niger KHNT-1 strain according to the present invention, steaming utilizing pottery as a steamer, and drying (fermentation) utilizing a medical LED as a dryer/fermenter, the method for improving the preparation of fermented black color ginseng is economical and safe since the preparation process takes 7 to 15 days and benzopyrene is not detected in the fermented black color ginseng, and the fermented black color ginseng contains ginsenosides Rg3, Rg5, and Rk1, and CK at high contents, and can be usefully utilized as a high value-added new drug raw material that is required to be contained at a high content per unit and in the field of high-functional food development.

公开(公告)号：US11932860B2

公开(公告)日：2024-03-19

申请号：US16652805

申请日：2018-10-03

申请人： Kikkoman Corporation

发明人： Junya Ishikawa

IPC分类号： C12N15/80 ,  C12N1/14 ,  C12R1/665 ,  C12R1/685 ,  C12R1/69

CPC分类号： C12N15/80 ,  C12N1/145 ,  C12R2001/665 ,  C12R2001/685 ,  C12R2001/69 ,  C12Y301/03001

摘要： The present invention provides a method for producing an alkaline phosphatase (ALP), the method including a step of: culturing an Aspergillus transformant capable of producing the ALP.

公开(公告)号：US20230167471A1

公开(公告)日：2023-06-01

申请号：US17744782

申请日：2022-05-16

申请人： Nanjing Haohe Biotechnology Co., Ltd.

发明人： Hao LIU ,  Qing XU

IPC分类号： C12P7/46 ,  C12N1/14 ,  C12N15/80

CPC分类号： C12P7/46 ,  C12N1/145 ,  C12N15/80 ,  C12N2800/10 ,  C12R2001/685

摘要： The disclosure discloses an Aspergillus niger engineered strain for reducing byproduct fumaric acid in a fermentation process of L-malic acid. The Aspergillus niger engineered strain is an Aspergillus niger engineered strain in which a fumarate hydratase gene fum is knocked out. The disclosure overcomes the defects in the prior art, in the current process of producing malic acid through fermentation of Aspergillus niger, byproduct fumaric acid can be accumulated with the generation of malic acid so as to cause the improved cost of the subsequent malic acid purification process. The disclosure provides an Aspergillus niger engineered strain in which a fum gene is knocked out and a method for greatly reducing byproduct fumaric acid in the fermentation production of Aspergillus niger.