公开(公告)号：US12195776B2

公开(公告)日：2025-01-14

申请号：US17547836

申请日：2021-12-10

Applicant: Novozymes, Inc.

Inventor： Jeffrey Shasky ,  Amanda Fischer ,  Suchindra Maiyuran

IPC: C12P7/06 ,  C07K14/38 ,  C12N9/24 ,  C12N9/38 ,  C12N9/42 ,  C12N15/52 ,  C12N15/80 ,  C12P7/16 ,  C12P19/02 ,  C12P19/14

Abstract: The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

公开(公告)号：US11805792B2

公开(公告)日：2023-11-07

申请号：US17550300

申请日：2021-12-14

Applicant: AGRIVIDA, INC.

Inventor： R. Michael Raab ,  Gabor Lazar ,  Binzhang Shen

IPC: A23K10/14 ,  C07K14/245 ,  C07K14/38 ,  C07K14/39 ,  C12N9/16 ,  C12N15/80 ,  A23K20/189 ,  C12N15/82 ,  C07K14/00

CPC classification number: A23K10/14 ,  A23K20/189 ,  C07K14/00 ,  C07K14/245 ,  C07K14/38 ,  C07K14/39 ,  C12N9/16 ,  C12N15/80 ,  C12N15/8257 ,  C12Y301/03

Abstract: Methods for enhancing phytase thermal stability by fusing binding elements to target phytases are provided. Engineered phytases that include binding elements fused to target phytases to cause cyclization of the engineered phytases and enhance thermal stability of the target phytases are described. Engineered nucleic acids encoding engineered phytases and hosts engineered to express engineered nucleic acids are also provided. Methods for incorporating engineered phytases in animal feed and animal feed including the same are described.

公开(公告)号：US20230135464A1

公开(公告)日：2023-05-04

申请号：US17642592

申请日：2020-09-11

Applicant: THE TRUSTEES OF DARTMOUTH COLLEGE

Inventor： Robert A. Cramer, Jr. ,  Caitlin Kowalski

IPC: C12N15/80 ,  C12N15/52 ,  C07K14/38

Abstract: Filamentous fungal host cells expressing hypoxia responsive morphology factor A (hrmA) and biofilm architecture factor (baf) proteins are provided. Methods of producing filamentous fungal host cells expressing hrmA and baf proteins are also provided. In one aspect, the disclosure provides a filamentous fungal host cell, comprising a nucleotide sequence encoding an Aspergillus fumigatus hypoxia responsive morphology factor A (hrmA) protein, or a homolog or ortholog thereof.

公开(公告)号：US11230723B2

公开(公告)日：2022-01-25

申请号：US16878267

申请日：2020-05-19

Applicant: Novozymes, Inc.

Inventor： Jeffrey Shasky ,  Suchindra Maiyuran ,  Amanda Fischer

IPC: C12P19/02 ,  C07K14/38 ,  C12P19/14 ,  C12N9/42

Abstract: The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

公开(公告)号：US20210388411A1

公开(公告)日：2021-12-16

申请号：US17263562

申请日：2019-07-13

Applicant: FEED RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

Inventor： Bin YAO ,  Xiaoyun SU ,  Fei GAO ,  Huiying LUO ,  Huoqing HUANG ,  Yingguo BAI ,  Yuan WANG ,  Tao TU ,  Yaru WANG ,  Kun MENG

IPC: C12Q1/40 ,  C12N15/80 ,  C07K14/38 ,  C07K14/435 ,  G01N15/14

Abstract: The present invention relates to the field of genetic engineering, particularly to a recombinant expression vector for rapidly screening the high expression strains and a method for rapidly screening high expression strains. In the invention, an exogenous red fluorescent protein and Aspergillus fumigatus cell surface protein localization signal are fused and expressed, and the fusion gene (DsRed-AfMP1) is integrated into the genome of Trichoderma reesei, so as to construct a strain displaying red fluorescent protein on the surface of Trichoderma reesei. By sorting Trichoderma reesei strains with red fluorescent protein on the surface by flow cytometry, genes beneficial to the improvement of cellulase activity can be quickly isolated.

公开(公告)号：US11085061B2

公开(公告)日：2021-08-10

申请号：US16395105

申请日：2019-04-25

Applicant: Novozymes, Inc. ,  Novozymes North America, Inc.

Inventor： Jason Quinlan ,  Feng Xu ,  Matt Sweeney ,  Don Higgins ,  Hui Xu

IPC: C12P19/14 ,  C12N9/42 ,  C12P7/10 ,  C12P7/28 ,  C12P7/40 ,  C07D495/04 ,  H01L51/00 ,  H01L51/42 ,  C12P13/04 ,  C12P19/02 ,  C12P19/12 ,  C12P5/00 ,  C12P5/02 ,  C12P7/26 ,  C12N9/96 ,  C07K14/37 ,  C07K14/38 ,  C07K14/385 ,  C07D333/52 ,  C07D333/72 ,  C07D333/76

Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a liquor. The present invention also relates to methods of using the compositions.

公开(公告)号：US10973242B2

公开(公告)日：2021-04-13

申请号：US16406801

申请日：2019-05-08

Applicant: WILD EARTH, INC.

Inventor： Ron Shigeta ,  Ryan Bethencourt ,  Abril Estrada

IPC: A23K20/147 ,  A23K10/16 ,  C07K14/37 ,  C07K14/395 ,  C07K14/38 ,  A23K20/158 ,  A23K10/18 ,  A23K40/00 ,  A23J1/00

Abstract: The present disclosure provides a composition comprising (i) at least about 20% protein by dry weight, wherein said protein is not animal-derived protein, (ii) at least 20% by dry weight intact yeast cells, (iii) 20% to 70% plant-derived flour, (iv) at least 15% plant-derived starch selected from amylose, amylopectin and a combination thereof, (v) a vegetable oil, and (vi) a nutritional supplement comprising a plurality of vitamins and a plurality of minerals, wherein said composition has a water content that is less than or equal to about 30% water by weight, and wherein the product is produced by heat expanded extrusion.

公开(公告)号：US20200325465A1

公开(公告)日：2020-10-15

申请号：US16864098

申请日：2020-04-30

Applicant: MARA Renewables Corporation

Inventor： Alexandra Merkx-Jacques ,  David Woodhall ,  Mark Scaife ,  Roberto E. Armenta ,  Denise Muise ,  Holly Rasmussen ,  Jeremy Benjamin

IPC: C12N9/92 ,  C12N9/12 ,  C07K14/40 ,  C12P7/64 ,  C07K14/38 ,  C12N1/12 ,  C12N1/10

Abstract: Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.

公开(公告)号：US10669550B2

公开(公告)日：2020-06-02

申请号：US16260032

申请日：2019-01-28

Applicant: Jiangnan University

Inventor： Long Liu ,  Jian Chen ,  Guocheng Du ,  Xian Yin ,  Jianghua Li

IPC: C12N15/80 ,  C07K14/38 ,  C12N15/63 ,  C12N1/14

Abstract: The invention discloses a promoter which can be induced to express in acidic conditions, and relates to the field of bioengineering technology. The promoters of the invention are separated from A. niger and can actuate and/or regulate the expression of the effectively connected nucleic acids in A. niger. In the invention the expression of the promoters is studied in A. niger, and it is indicated that some promoters show weak expression, and some show strong activity. The invention provides an effective method and new thought for organic acids production by fungi or other products produced by fermentation under acidic conditions.

公开(公告)号：US20200063166A1

公开(公告)日：2020-02-27

申请号：US16493017

申请日：2018-03-12

Applicant: DSM IP Assets B.V.

Inventor： Noeel Nicolaas Maria Elisabeth VAN PEIJ ,  Peter Jozef Ida VAN DE VONDERVOORT

IPC: C12P1/02 ,  C07K14/38 ,  C12N15/80

Abstract: The present disclosure relates to a mutant filamentous fungal host cell which is deficient in the zinc binuclear cluster transcriptional regulator oreR or in a functional homologue thereof if compared with a parent filamentous fungal host cell which has not been modified and measured under the same conditions. It has been surprisingly found that when the mutant filamentous fungal host cell according to the disclosure is used in a method to produce a compound of interest by microbial fermentation, for example an enzyme, substantially no oxalic acid is produced extracellularly by the cell during the fermentation as a by-product, which allows a more economical and efficient recovery of the compound of interest from the fermentation broth.