Variants of Glycoside Hydrolases
    4.
    发明申请

    公开(公告)号:US20130130353A1

    公开(公告)日:2013-05-23

    申请号:US13748328

    申请日:2013-01-23

    申请人: Novozymes, Inc.

    IPC分类号: C12N9/24

    摘要: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    Variants of glycoside hydrolases
    5.
    发明授权
    Variants of glycoside hydrolases 有权
    糖苷水解酶的变体

    公开(公告)号:US07932073B2

    公开(公告)日:2011-04-26

    申请号:US11891249

    申请日:2007-08-08

    IPC分类号: C12N9/42 C12S3/00 C11D3/00

    摘要: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    摘要翻译: 本发明涉及母体糖苷水解酶的变体,其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代,和 486,SEQ ID NO:2的氨基酸1至513,并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代, SEQ ID NO:2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113, SEQ ID NO:2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462,其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体,载体和宿主细胞。