公开(公告)号：US20170298363A1

公开(公告)日：2017-10-19

申请号：US15511833

申请日：2015-09-18

申请人： GENOMATICA, INC

发明人： Priti Pharkya ,  Anthony Burgard ,  Eric Roland Nunez Van Name

IPC分类号： C12N15/52 ,  C12N9/12 ,  C12P7/18 ,  C12P5/02 ,  C12N9/88 ,  C12P7/40 ,  C12P7/04

CPC分类号： C12N15/52 ,  C12N9/1205 ,  C12N9/88 ,  C12P5/026 ,  C12P7/04 ,  C12P7/18 ,  C12P7/40 ,  C12P7/42 ,  C12Y207/0104 ,  C12Y401/02009 ,  C12Y401/02022 ,  Y02E50/343

摘要： The invention provides non-natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA, or oxaloacetate and acetyl-CoA. Embodiments of the invention include microbial organisms having a pathway to acetyl-CoA and oxaloacetate that includes phosphoketolase (a PK pathway). The organisms also have either (i) a genetic modification that enhances the activity of the non-phosphotransferase system (non-PTS) for sugar uptake, and/or (ii) a genetic modification(s) to the organism's electron transport chain (ETC) that enhances efficiency of ATP production, that enhances availability of reducing equivalents or both. The microbial organisms can optionally include (iii) a genetic modification that maintains, attenuates, or eliminates the activity of a phosphotransferase system (PTS) for sugar uptake. The enhanced carbon flux through acetyl-CoA and oxaloacetate can be used for production of a bioderived compound, and the microbial organisms can further include a pathway capable of producing the bioderived compound.

公开(公告)号：US20150204874A1

公开(公告)日：2015-07-23

申请号：US14417023

申请日：2013-07-26

申请人： Joslin Diabetes Center, Inc.

发明人： George L. King ,  Hillary A. Keenan

IPC分类号： G01N33/573 ,  A61K38/45 ,  A61K45/06 ,  C12N15/113

CPC分类号： G01N33/573 ,  A61K38/45 ,  A61K45/06 ,  C12N15/1137 ,  C12N2310/122 ,  C12N2310/14 ,  C12Q1/6883 ,  C12Q2600/158 ,  C12Y207/0104 ,  G01N2333/91215 ,  G01N2800/042 ,  G01N2800/347 ,  G01N2800/52 ,  G01N2800/56

摘要： Compositions and methods for diagnosing, predicting risk of and/or treating diabetic nephropathy (DN).

摘要翻译： 用于诊断，预测和/或治疗糖尿病肾病（DN）风险的组合物和方法。

公开(公告)号：US20140193869A1

公开(公告)日：2014-07-10

申请号：US14137524

申请日：2013-12-20

申请人： GreenLight Biosciences, Inc.

发明人： William Jeremy Blake ,  James R. Swartz

IPC分类号： C12P7/16

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0073 ,  C12N9/1022 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/88 ,  C12N9/90 ,  C12N15/70 ,  C12P7/16 ,  C12P7/40 ,  C12P7/649 ,  C12Y101/01244 ,  C12Y102/01012 ,  C12Y114/13025 ,  C12Y202/01001 ,  C12Y207/01011 ,  C12Y207/0104 ,  C12Y207/02003 ,  C12Y401/02013 ,  C12Y401/02043 ,  C12Y402/01011 ,  C12Y501/03001 ,  C12Y503/01001 ,  C12Y503/01027 ,  Y02E50/10 ,  Y02E50/13

摘要： The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.

摘要翻译： 在一些方面，本公开涉及用于甲烷大规模转化为异丁醇的无细胞方法和系统，其包括在升高的压力下在生物反应器中组合甲烷，氧气和含有甲烷单加氧酶，甲醇脱氢酶， 以及催化甲醛转化为异丁醇的酶，形成无细胞的反应混合物，并在合适的条件下孵育将甲烷转化为异丁醇的无细胞反应。

公开(公告)号：US08426676B2

公开(公告)日：2013-04-23

申请号：US12598611

申请日：2008-04-29

申请人： Oliver Oswald ,  Heiko A. Härtel ,  Christoph Benning ,  Carl Andre

发明人： Oliver Oswald ,  Heiko A. Härtel ,  Christoph Benning ,  Carl Andre

IPC分类号： A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C07H21/04

CPC分类号： C12Y207/0104 ,  C12N9/1205 ,  C12N15/8247 ,  C12N15/8251

摘要： Described herein are inventions in the field of genetic engineering of plants, including combinations of nucleic acid molecules encoding pyruvate kinase subunits to improve agronomic, horticultural, and quality traits. This invention relates generally to the combination of nucleic acid sequences encoding pyruvate kinase proteins that are related to the presence of seed storage compounds in plants. More specifically, the present invention relates to the use of these combinations of these sequences, their order and direction in the combination, and the regulatory elements used to control expression and transcript termination in these combinations in transgenic plants. In particular, the invention is directed to methods for manipulating seed storage compounds in plants and seeds. The invention further relates to methods of using these novel combinations of polypeptides to stimulate plant growth and/or root growth and/or to increase yield and/or composition of seed storage compounds.

摘要翻译： 本文描述了植物遗传工程领域的发明，包括编码丙酮酸激酶亚基的核酸分子的组合，以改善农艺，园艺和品质性状。 本发明一般涉及编码丙酮酸激酶蛋白的核酸序列的组合，其与植物中种子储存化合物的存在有关。 更具体地，本发明涉及这些序列的这些组合的用途，它们在组合中的顺序和方向，以及用于控制转基因植物中这些组合中的表达和转录终止的调节元件。 特别地，本发明涉及在植物和种子中操作种子储存化合物的方法。 本发明还涉及使用这些多肽的这些新型组合来刺激植物生长和/或根生长和/或增加种子储存化合物的产量和/或组成的方法。

公开(公告)号：US20040038417A1

公开(公告)日：2004-02-26

申请号：US10203334

申请日：2003-07-07

发明人： Dolores J. Cahill ,  Eckhard Bordhoff ,  Joachim Klose ,  Holger Eickhoff ,  Frank Schmidt ,  Hans Lehrach

IPC分类号： G01N033/00

CPC分类号： C12N9/0008 ,  C12N9/1205 ,  C12Y102/01012 ,  C12Y207/0104 ,  Y10T436/24

摘要： The present invention relates to a method for identifying and/or characterizing a (poly)peptide comprising: (a) analyzing a peptide map of said (poly)peptide, comprising at least 1 peptide, and its peptide primary structure fingerprint by mass spectrometry; and (b) comparing data obtained in step (a) with a reference (poly)peptide database, said database comprising mass spectrometric data of peptide maps, comprising at least 1 peptide, and of its peptide primary structure fingerprint, of a (poly)peptide or of a variety of (poly)peptides.

摘要翻译： 本发明涉及一种用于鉴定和/或表征（多）肽的方法，包括：（a）通过质谱分析所述（多）肽的肽图，其包含至少1个肽及其肽一级结构指纹; （b）将步骤（a）中获得的数据与参考（多）肽数据库进行比较，所述数据库包括肽图谱的质谱数据，其包含至少1个肽及其肽一级结构指纹的（聚） 肽或多种（多）肽。

公开(公告)号：US20240352495A1

公开(公告)日：2024-10-24

申请号：US18685259

申请日：2022-09-13

申请人： Moderna Enzymatics Co., Ltd.

发明人： Masayuki Suetsugu ,  Seia Nara

IPC分类号： C12P19/34 ,  C12N9/12 ,  C12N15/11

CPC分类号： C12P19/34 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/1223 ,  C12N9/1229 ,  C12N15/11 ,  C12Y207/0104 ,  C12Y207/02001 ,  C12Y207/03002 ,  C12Y207/04001 ,  C12Y207/04006

摘要： Provided is a method for producing circular DNA in which a region that is sandwiched by a region Ha and a region Hb in circular double-stranded DNA is substituted with the entirety or a portion of a linear-DNA fragment, wherein the region Hb is located downstream of the region Ha in the circular double-stranded DNA; the linear-DNA fragment is single-stranded or double-stranded linear DNA that has a homologous region that corresponds to the region Ha and a homologous region that corresponds to the region Hb, the latter homologous region being positioned downstream of the former homologous region; and the method comprising: preparing a reaction solution that contains the circular double-stranded DNA, the linear-DNA fragment, and a protein that has RecA-family-recombinase activity, and performing homologous recombination reaction by incubating the reaction solution for a predetermined period of time, thereby producing circular DNA in which the region that is from the region Ha to the region Hb in the circular double-stranded DNA is substituted with the region that is from the homologous region that corresponds to the region Ha to the homologous region that corresponds to the region Hb in the linear-DNA fragment.

公开(公告)号：US11717542B2

公开(公告)日：2023-08-08

申请号：US17433225

申请日：2020-02-20

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： Wei Kong ,  Lingchen Fu ,  Yixin Shi ,  Bo Ning

IPC分类号： A61K35/74 ,  A61P35/00 ,  C07K14/255 ,  C12N1/20 ,  C12N9/12

CPC分类号： A61K35/74 ,  A61P35/00 ,  C07K14/255 ,  C12N1/20 ,  C12N9/1205 ,  C12Y207/0104

摘要： The present disclosure relates to genetically modified strains of Salmonella, engineered to be tumor navigating and to alter tumor metabolism in the tumor microenvironment. Also provided herein are methods of producing and methods of using such genetically modified Salmonella strains to treat cancer.

公开(公告)号：US09932590B2

公开(公告)日：2018-04-03

申请号：US14902505

申请日：2014-07-02

申请人： THE UNIVERSITY COURT OF THE UNIVERSITY OF ABERDEEN ,  THE SECRETARY OF STATE FOR ENVIRONMENT, FOOD AND RURAL AFFAIRS

发明人： Alan Stuart Bowman ,  Ewan McInnes Campbell ,  Giles Elliott Budge

IPC分类号： C12N15/113 ,  A01N57/10 ,  A23K20/153 ,  A23K50/90 ,  A01N57/16

CPC分类号： C12N15/1137 ,  A01N57/16 ,  A23K20/153 ,  A23K50/90 ,  C12N15/113 ,  C12N15/1136 ,  C12N15/1138 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/51 ,  C12N2320/30 ,  C12N2320/31 ,  C12Y104/03004 ,  C12Y204/01016 ,  C12Y205/01018 ,  C12Y207/0104 ,  C12Y301/01007 ,  C12Y306/01003 ,  C12Y306/03014

摘要： Nucleic acid agents for reducing or removing infestations of the Varroa destructor mite are described. Compositions comprising the nucleic acid agents and methods for controlling mite infestations using the nucleic acid agents and compositions are also disclosed.

公开(公告)号：US20170360840A1

公开(公告)日：2017-12-21

申请号：US15625961

申请日：2017-06-16

申请人： United Therapeutics Corporation

发明人： Sarah HOGAN ,  Roger Marquez ILAGAN ,  Maria Pia RODRIGUEZ ,  Carolina MEDRANO ,  John CHEADLE

IPC分类号： A61K35/28 ,  C12N15/113 ,  A61K38/46 ,  C12N9/12 ,  C12N5/0775 ,  C12N9/14 ,  A61K38/45

CPC分类号： A61K35/28 ,  A61K38/45 ,  A61K38/46 ,  C12N5/0662 ,  C12N9/1205 ,  C12N9/14 ,  C12N15/113 ,  C12N2310/141 ,  C12Y207/0104 ,  C12Y306/01003

摘要： Provided are methods for isolating potent extracellular vesicle or exosome populations from mesenchymal stromal cells, and the use of the isolated extracellular vesicles or exosomes in treating vasculopathy, including pulmonary hypertension, bronchopulmonary dysplasia, and disease and conditions associated with mitochondrial dysfunction.

公开(公告)号：US20040115631A1

公开(公告)日：2004-06-17

申请号：US10311527

申请日：2003-11-26

发明人： Ulf R. Rapp ,  Erich Eigenbrodt

IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12N009/12

CPC分类号： C12N9/1205 ,  A61K38/00 ,  C12Y207/0104

摘要： The invention relates to a nucleid acid coding for at least one partial sequence of a protein kinase of the mitogenic signalling cascade, whereby the partial sequence codes for a binding site for a glycolysis-catalysing enzyme. The invention further relates to a silent mutation of one such nucleic acid or a nucleic acid hybridising with one such nucleic acid or the silent mutation thereof.

摘要翻译： 本发明涉及编码有丝分裂信号级联蛋白激酶的至少一部分序列的核酸，由此部分序列编码糖酵解催化酶的结合位点。 本发明还涉及一种这样的核酸的沉默突变或与一种这样的核酸杂交的核酸或其沉默突变。