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1.发明授权Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose 有权酵母表达纤维素酶用于同时糖化和发酵使用纤维素公开(公告)号：US09102955B2
公开(公告)日：2015-08-11
申请号：US13130549
申请日：2009-11-23
申请人： John McBride , Elena Brevnova , Mark Mellon , Allan Froehlich , Kristen Deleault , Vineet Rajgarhia , Riaan Den Haan , Merja Penttila , Marja Ilmen , Matti Siika-Aho , Jaana Uusitalo , Emily A. Stonehouse , Alan Gilbert , Haowen Xu , Deidre Willes , John Bardsley , Anu Koivula , Sanni Voutilainen
发明人： John McBride , Elena Brevnova , Mark Mellon , Allan Froehlich , Kristen Deleault , Vineet Rajgarhia , Riaan Den Haan , Merja Penttila , Marja Ilmen , Matti Siika-Aho , Jaana Uusitalo , Emily A. Stonehouse , Alan Gilbert , Haowen Xu , Deidre Willes , John Bardsley , Anu Koivula , Sanni Voutilainen
CPC分类号： C12P7/10 , C12N9/2437 , C12N9/2445 , C12N15/80 , C12P7/14 , C12P39/00 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.
摘要翻译： 包含表达异源纤维素酶的克氏杆菌的宿主细胞从纤维素产生乙醇此外，可以将表达不同杂种纤维素酶的多个宿主细胞共同共培养并用于从纤维素产生乙醇可以使用酵母菌株的重组酵母菌株和共培养物 自己生产乙醇，也可以与外加纤维素酶组合使用，以提高发酵和发酵过程的效率。
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公开(公告)号：US08765428B2
公开(公告)日：2014-07-01
申请号：US13054750
申请日：2009-07-17
CPC分类号： C12P7/10 , C08B37/0003 , C08B37/0057 , C08H8/00 , C12M21/12 , C12M27/02 , C12M29/04 , C12M45/09 , C12P19/14 , C13K1/02 , D21C3/22 , Y02E50/16 , Y02E50/17
摘要： The present invention is directed to a process for biologically converting carbohydrates from lignocellulosic biomass comprising the steps of: suspending lignocellulosic biomass in a flow-through reactor, passing a reaction solution into the reactor, wherein the solution is absorbed into the biomass substrate and at least a portion of the solution migrates through said biomass substrate to a liquid reservoir, recirculating the reaction solution in the liquid reservoir at least once to be absorbed into and migrate through the biomass substrate again. The biological converting of the may involve hydrolyzing cellulose, hemicellulose, or a combination thereof to form oligosaccharides, monomelic sugars, or a combination thereof; fermenting oligosaccharides, monomelic sugars, or a combination thereof to produce ethanol, or a combination thereof. The process can further comprise removing the reaction solution and processing the solution to separate the ethanol produced from non-fermented solids.
摘要翻译： 本发明涉及一种用于从木质纤维素生物质生物转化碳水化合物的方法，包括以下步骤：将木质纤维素生物质悬浮在流通反应器中，使反应溶液通入反应器，其中溶液被吸收到生物质底物中，并且至少 溶液的一部分通过所述生物质基质移动到液体储存器，使液体储存器中的反应溶液至少再循环一次，以再次吸收进入生物质基质并通过生物质基质迁移。 生物转化可能包括水解纤维素，半纤维素或其组合以形成寡糖，单糖或其组合; 发酵寡糖，单体糖或其组合以产生乙醇，或其组合。 该方法还可以包括除去反应溶液并处理溶液以分离由非发酵固体产生的乙醇。
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公开(公告)号：US20120028325A1
公开(公告)日：2012-02-02
申请号：US13054750
申请日：2009-07-17
IPC分类号： C12P7/10
CPC分类号： C12P7/10 , C08B37/0003 , C08B37/0057 , C08H8/00 , C12M21/12 , C12M27/02 , C12M29/04 , C12M45/09 , C12P19/14 , C13K1/02 , D21C3/22 , Y02E50/16 , Y02E50/17
摘要： The present invention is directed to a process for biologically converting carbohydrates from lignocellulosic biomass comprising the steps of: suspending lignocellulosic biomass in a flow-through reactor, passing a reaction solution into the reactor, wherein the solution is absorbed into the biomass substrate and at least a portion of the solution migrates through said biomass substrate to a liquid reservoir, recirculating the reaction solution in the liquid reservoir at least once to be absorbed into and migrate through the biomass substrate again. The biological converting of the may involve hydrolyzing cellulose, hemicellulose, or a combination thereof to form oligosaccharides, monomelic sugars, or a combination thereof; fermenting oligosaccharides, monomelic sugars, or a combination thereof to produce ethanol, or a combination thereof. The process can further comprise removing the reaction solution and processing the solution to separate the ethanol produced from non-fermented solids.
摘要翻译： 本发明涉及一种用于从木质纤维素生物质生物转化碳水化合物的方法，包括以下步骤：将木质纤维素生物质悬浮在流通反应器中，使反应溶液通入反应器，其中溶液被吸收到生物质底物中，并且至少 溶液的一部分通过所述生物质基质移动到液体储存器，使液体储存器中的反应溶液至少再循环一次，以再次吸收进入生物质基质并通过生物质基质迁移。 生物转化可能包括水解纤维素，半纤维素或其组合以形成寡糖，单糖或其组合; 发酵寡糖，单体糖或其组合以产生乙醇，或其组合。 该方法还可以包括除去反应溶液并处理溶液以分离由非发酵固体产生的乙醇。
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4.发明申请Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol 审中-公开用于将木质纤维素生物质转化为乙醇的嗜热生物体公开(公告)号：US20090221049A1
公开(公告)日：2009-09-03
申请号：US12299070
申请日：2007-05-01
申请人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin , Kara Podkaminer , John Bardsley , David Anthony Hogsett
发明人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin , Kara Podkaminer , John Bardsley , David Anthony Hogsett
CPC分类号： C12N9/1029 , C12N9/0006 , C12N9/1217 , C12P7/10 , C12R1/145 , C12Y203/01008 , C12Y207/02001 , Y02E50/16 , Y02E50/17
摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.
摘要翻译： 本文公开了消耗多种生物质衍生底物的突变嗜热生物。 本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。 此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。 涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。 生物可以用于例如在对于纤维素酶活性最佳以在不表达丙酮酸脱羧酶的情况下产生接近理论乙醇产生的温度下进行的嗜热SSF和SSCF反应。
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公开(公告)号：US5761072A
公开(公告)日：1998-06-02
申请号：US555572
申请日：1995-11-08
CPC分类号： G01R31/3613 , G01R31/3651
摘要： A microprocessor samples the net effective current from a battery several times a second. A fast average current I.sub.f is calculated using an IIR filter on the net average current. A slow average current I is calculated by integrating the net average current over time with respect to time. An Ampere Hour Capacity is calculated by the use of the Peukert equation, which is split into two terms. A first capacity term is calculated using the fast average current I.sub.f and a slow capacity term is calculated using the slow average current I. The slow capacity term is then multiplied by a weighting constant C.sub.2 and summed with the fast capacity term which is multiplied by (1-C.sub.2).
摘要翻译： 微处理器每秒将电池的净有效电流多次采样一次。 使用IIR滤波器对净平均电流计算快速平均电流If。 通过将净平均电流随时间相对于时间积分来计算慢平均电流+ E，ovs I + EE。 安培小时容量是通过使用Peukert方程式计算的，分为两个术语。 使用快速平均电流If计算第一容量项，并使用慢平均电流+ E，ovs I + EE计算慢容量项。 然后将慢容量项乘以加权常数C2，并与乘以（1-C2）的快速容量项相加。
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公开(公告)号：US20110171709A1
公开(公告)日：2011-07-14
申请号：US12740839
申请日：2008-10-29
申请人： John Bardsley
发明人： John Bardsley
IPC分类号： C12P7/10
摘要： The present invention is directed to a process of producing ethanol from lignocellulosic biomass, which comprises pre-treating a lignocellulosic feedstock to produce a reactive carbohydrate mixture; adding activated carbon in free form; converting said reactive carbohydrate mixture to form a beer; separating solids from said carbohydrate mixture or said beer or both, wherein said activated carbon is separated along with the solids in said mixture, said beer or both; and drying said solids. The invention is also directed to the production of a dried solid fuel to be combusted during said process.
摘要翻译： 本发明涉及从木质纤维素生物质生产乙醇的方法，其包括预处理木质纤维素原料以产生反应性碳水化合物混合物; 添加游离形式的活性炭; 转化所述活性碳水化合物混合物以形成啤酒; 从所述碳水化合物混合物或所述啤酒或两者分离固体，其中所述活性炭与所述混合物中的固体，所述啤酒或两者分离; 并干燥所述固体。 本发明还涉及在所述方法中生产待燃烧的干燥固体燃料。
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公开(公告)号：US10066217B2
公开(公告)日：2018-09-04
申请号：US12299070
申请日：2007-05-01
申请人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Kara Podkaminer , John Bardsley , David Anthony Hogsett
发明人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Kara Podkaminer , John Bardsley , David Anthony Hogsett
摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.
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8.发明申请Yeast Expressing Cellulases for Simultaneous Saccharification and Fermentation Using Cellulose 有权酵母表达纤维素酶用于同时糖化和发酵使用纤维素公开(公告)号：US20120129229A1
公开(公告)日：2012-05-24
申请号：US13130549
申请日：2009-11-23
申请人： John McBride , Elena Brevnova , Mark Mellon , Allan Froehlich , Kristen Deleault , Vineet Rajgarhia , Riaan Den Haan , Merja Penttila , Marja Ilmen , Matti Siika-Aho , Jaana Uusitalo , Emily A. Stonehouse , Alan Gilbert , Haowen Xu , Deidre Willes , John Bardsley , Anu Koivula , Sanni Voutilainen
发明人： John McBride , Elena Brevnova , Mark Mellon , Allan Froehlich , Kristen Deleault , Vineet Rajgarhia , Riaan Den Haan , Merja Penttila , Marja Ilmen , Matti Siika-Aho , Jaana Uusitalo , Emily A. Stonehouse , Alan Gilbert , Haowen Xu , Deidre Willes , John Bardsley , Anu Koivula , Sanni Voutilainen
CPC分类号： C12P7/10 , C12N9/2437 , C12N9/2445 , C12N15/80 , C12P7/14 , C12P39/00 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes
摘要翻译： 包含表达异源纤维素酶的克氏杆菌的宿主细胞从纤维素产生乙醇此外，可以将表达不同杂种纤维素酶的多个宿主细胞共同共培养并用于从纤维素产生乙醇可以使用酵母菌株的重组酵母菌株和共培养物 自己生产乙醇，也可以与外加的纤维素酶组合使用，以提高发酵和发酵过程的效率
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9.发明申请Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol 审中-公开用于将木质纤维素生物质转化为乙醇的嗜热生物体公开(公告)号：US20120077239A9
公开(公告)日：2012-03-29
申请号：US12299070
申请日：2007-05-01
申请人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin , Kara Podkaminer , John Bardsley , David Anthony Hogsett
发明人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin , Kara Podkaminer , John Bardsley , David Anthony Hogsett
CPC分类号： C12N9/1029 , C12N9/0006 , C12N9/1217 , C12P7/10 , C12R1/145 , C12Y203/01008 , C12Y207/02001 , Y02E50/16 , Y02E50/17
摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.
摘要翻译： 本文公开了消耗多种生物质衍生底物的突变嗜热生物。 本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。 此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。 涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。 生物可以用于例如在对于纤维素酶活性最佳以在不表达丙酮酸脱羧酶的情况下产生接近理论乙醇产生的温度下进行的嗜热SSF和SSCF反应。
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