公开(公告)号：US20240076659A1

公开(公告)日：2024-03-07

申请号：US18261083

申请日：2022-07-29

Applicant: 4BASEBIO, S.L.U. ,  4BASEBIO UK LTD

Inventor： Ángel Picher ,  Heikki Lanckriet ,  Amy Walker

IPC: C12N15/11 ,  C12N15/86 ,  C12Q1/6848

CPC classification number: C12N15/11 ,  C12N15/86 ,  C12Q1/6848 ,  C12N2310/122 ,  C12N2310/315

Abstract: Methods for producing a linear deoxyribonucleic acid (DNA) product with enhanced resistance to nuclease digestion are provided. The methods comprise: (a) contacting a double-stranded DNA molecule with an endonuclease, a ligase and first and second adaptor molecules to form a single contiguous aqueous volume; and (b) incubating the single contiguous aqueous volume to generate a linear DNA product. There are also provided linear deoxyribonucleic acid (DNA) products with enhanced resistance to nuclease digestion and uses thereof.

公开(公告)号：US20240360488A1

公开(公告)日：2024-10-31

申请号：US18429780

申请日：2024-02-01

Applicant: 4BASEBIO, S.L.U. ,  4BASEBIO UK LTD

Inventor： Heikki Lanckriet ,  Amy Walker ,  Ángel Picher

IPC: C12P19/34 ,  C12N9/22 ,  C12N15/10

CPC classification number: C12P19/34 ,  C12N9/22 ,  C12N15/10 ,  C12N2310/122 ,  C12N2310/315 ,  C12Y207/07 ,  C12Y301/11001 ,  C12Y301/11002

Abstract: Methods for producing a linear deoxyribonucleic acid (DNA) product with enhanced resistance to nuclease digestion are provided. The methods comprise, (a) digesting a double-stranded DNA molecule with an endonuclease that cleaves an endonuclease target sequence to generate a digested double-stranded DNA molecule, wherein the digested double-stranded DNA molecule comprises a linear double-stranded region, and a truncated protelomerase sequence at a first end, wherein the truncated protelomerase target sequence is non-functional; (b) appending a first adaptor molecule to the first end of the digested double-stranded DNA molecule and appending a second adaptor molecule to the second end of the digested double-stranded DNA molecule to generate a precursor double-stranded DNA molecule, wherein the first adaptor molecule comprises a truncated protelomerase target sequence that forms a first functional protelomerase target sequence with the truncated protelomerase sequence at the first end of the digested double-stranded DNA molecule; and (c) incubating the precursor double-stranded DNA molecule with a protelomerase to generate the linear DNA product, wherein the protelomerase closes the first end of the precursor double-stranded DNA molecule at the first functional protelomerase target sequence.

公开(公告)号：US20240358846A1

公开(公告)日：2024-10-31

申请号：US18291825

申请日：2022-07-29

Applicant: 4BASEBIO, S.L.U. ,  4BASEBIO UK LTD

Inventor： Amy Walker ,  Heikki Lanckriet ,  Ángel Picher ,  Elena Dyankova

IPC: A61K47/66 ,  A61K9/51 ,  A61K47/69 ,  C07K7/06 ,  C07K7/08 ,  C12N15/88

CPC classification number: A61K47/66 ,  A61K9/5123 ,  A61K9/5169 ,  A61K47/6911 ,  C07K7/06 ,  C07K7/08 ,  C12N15/88

Abstract: Nanoparticles suitable for delivery of a cargo to a muscle cell, and targeting peptides comprising a muscle cell targeting sequence, are provided. Further provided are uses of the nanoparticles and targeting peptides, for example, in treating a muscle disease or disorder such as a skeletal muscle disease or disorder or a heart disease or disorder.

公开(公告)号：US20240209362A1

公开(公告)日：2024-06-27

申请号：US18288545

申请日：2022-04-29

Applicant: 4BASEBIO, S.L.U. ,  4BASEBIO UK LTD

Inventor： Heikki Lanckriet ,  Ángel Picher ,  Amy Walker

IPC: C12N15/113 ,  C12N15/10

CPC classification number: C12N15/113 ,  C12N15/102 ,  C12N15/1082 ,  C12N2310/11 ,  C12N2310/315

Abstract: The present invention relates to linear double-stranded DNA products comprising nuclease-resistant nucleotides at internal positions in each strand. In addition, the present invention relates to complex molecules, nanoparticles, compositions and libraries comprising the linear double-stranded DNA products. Also provided are methods for producing and using the linear double-stranded DNA products.