公开(公告)号：US20250019864A1

公开(公告)日：2025-01-16

申请号：US18606205

申请日：2024-03-15

Applicant: Life Technologies Corporation

Inventor： Timothy Looney ,  Geoffrey Lowman ,  Lifeng Lin

IPC: C40B50/06 ,  C07K14/725 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US10920273B2

公开(公告)日：2021-02-16

申请号：US15873862

申请日：2018-01-17

Applicant: Life Technologies Corporation

Inventor： Timothy Looney ,  Geoffrey Lowman ,  Lifeng Lin

IPC: C40B50/06 ,  C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12Q1/6811 ,  C12Q1/6881 ,  C12Q1/6883 ,  C07K14/725

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20230416810A1

公开(公告)日：2023-12-28

申请号：US18317484

申请日：2023-05-15

Applicant: Life Technologies Corporation

Inventor： Geoffrey Lowman ,  Chenchen Yang ,  Timothy Looney

IPC: C12Q1/6844 ,  C12Q1/6883

CPC classification number: C12Q1/6844 ,  C12Q1/6883 ,  C12Q2600/16

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of B cell receptor heavy and light chains in a single assay, with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20230340602A1

公开(公告)日：2023-10-26

申请号：US18318402

申请日：2023-05-16

Applicant: Life Technologies Corporation

Inventor： Geoffrey Lowman ,  Chenchen Yang ,  Timothy Looney

IPC: C12Q1/6883 ,  C12Q1/6869 ,  G16B50/30 ,  G16B40/10

CPC classification number: C12Q1/6883 ,  C12Q1/6869 ,  G16B50/30 ,  G16B40/10 ,  C12Q2600/112

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, methods provide for determining convergence of T cell receptor beta and T cell receptor gamma repertoires in samples prior to a treatment and predicting a subject's response to the treatment. In another aspect, methods provide predicting a subject's potential or predisposition to be protected from or vulnerable to an adverse event following a treatment.

公开(公告)号：US11008609B2

公开(公告)日：2021-05-18

申请号：US16120045

申请日：2018-08-31

Applicant: Life Technologies Corporation

Inventor： Geoffrey Lowman ,  Timothy Looney ,  Lifeng Lin ,  Elizabeth Linch ,  Lauren Miller

IPC: C40B50/06 ,  C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6869 ,  C40B50/00

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire using genomic DNA from a biological sample. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Nucleic acid sequences of variable regions associated with the immune cell receptor are determined to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US12227878B2

公开(公告)日：2025-02-18

申请号：US17302638

申请日：2021-05-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Geoffrey Lowman ,  Timothy Looney ,  Lifeng Lin ,  Elizabeth Linch ,  Lauren Miller

IPC: C40B40/08 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6869 ,  C40B50/00 ,  C40B50/06

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire using genomic DNA from a biological sample. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Nucleic acid sequences of variable regions associated with the immune cell receptor are determined to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US11970787B2

公开(公告)日：2024-04-30

申请号：US17248722

申请日：2021-02-04

Applicant: Life Technologies Corporation

Inventor： Timothy Looney ,  Geoffrey Lowman ,  Lifeng Lin

IPC: C40B50/06 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883 ,  C07K14/725

CPC classification number: C40B50/06 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883 ,  C07K14/7051 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/166

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.