公开(公告)号：US11686014B2

公开(公告)日：2023-06-27

申请号：US16636523

申请日：2018-08-06

Applicant: CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Kwang-Hyun Baek ,  So-Ra Kim ,  Seul-Ki Kwon ,  Soo-Yeon Kim ,  Da-Hye Lee

IPC: C40B30/06 ,  C12N9/48 ,  C12N15/10 ,  C12N15/81 ,  C12Q1/37 ,  C40B40/02 ,  C40B40/08

CPC classification number: C40B30/06 ,  C12N9/48 ,  C12N15/1055 ,  C12N15/81 ,  C12Q1/37 ,  C40B40/02 ,  C40B40/08

Abstract: The present invention provides a vector library for yeast two-hybrid screening of a deubiquitinating enzyme that binds to a target protein and a method for identifying a deubiquitinating enzyme binding to a target protein using the same. Also, the present invention provides a method for screening an agent having anti-cancer activity targeting the deubiquitinating enzyme USP1, USP7, USP12, or USP49 identified by said identifying method.

公开(公告)号：US12098174B2

公开(公告)日：2024-09-24

申请号：US17310517

申请日：2020-02-06

Applicant: CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Kwang-Hyun Baek ,  Ji-Hye Choi

IPC: C07K14/47

CPC classification number: C07K14/4702

Abstract: The present invention provides an Oct-3/4 protein variant wherein lysine at position 156; lysine at position 286; or lysines at positions 156 and 286 in the Oct-3/4 protein is (are) substituted with arginine. The Oct-3/4 protein variant according to the present invention can inhibit a protein degradation mechanism through ubiquitination, and shows a higher half-life, compared to the wild-type Oct-3/4, thereby exhibiting high stability.