知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

194 条记录 (耗时 0.03 秒)

    Method of immobilising RNA onto a surface
    3.
    发明授权
    Method of immobilising RNA onto a surface 有权

    公开(公告)号：US09777268B2

    公开(公告)日：2017-10-03

    申请号：US14116390

    申请日：2012-05-14

    申请人： Anastasia Jane Callaghan

    发明人： Anastasia Jane Callaghan

    IPC分类号： C12Q1/68 ,  C12N15/10 ,  C12N15/113

    CPC分类号： C12N15/1093 ,  C12Q1/6837 ,  C12Q2525/205 ,  C12Q2563/131 ,  C12Q2565/515 ,  C12Q2521/119 ,  C12Q2525/143

    摘要： The invention relates to a method of immobilising at least one RNA molecule onto a surface of a support comprising: i) providing a first support having a surface on which at least one DNA molecule is immobilised, wherein the DNA molecule encodes an RNA molecule and the encoded RNA molecule comprises a binding molecule; ii) providing a second support having a surface on which at least one binding partner for interacting with the binding molecule is immobilised; iii) arranging the first and second supports such that the surfaces displaying the immobilised molecules are in close proximity and substantially face each other, and contacting the DNA molecule immobilised on the surface of the first support with transcription reagents; and iv) carrying out a transcription reaction to generate the encoded RNA molecule, wherein the RNA molecule is directly immobilised onto the surface of the second support via an interaction between the binding molecule of the RNA molecule and the binding partner on the surface of the second support.

    COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FROM COMPLEX MIXTURES USING HYBRIDIZATION
    5.
    发明申请
    COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FROM COMPLEX MIXTURES USING HYBRIDIZATION 有权

    公开(公告)号：US20150353998A1

    公开(公告)日：2015-12-10

    申请号：US14829152

    申请日：2015-08-18

    申请人： Illumina, Inc.

    发明人： John R. Stuelpnagel ,  David L. Barker ,  Jorge Velarde ,  Steven M. Barnard ,  Michael Graige

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1072 ,  C12Q1/6837 ,  C12Q2565/515

    摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.

    Method and arrays for detecting biological molecules
    6.
    发明授权
    Method and arrays for detecting biological molecules 有权
    用于检测生物分子的方法和阵列

    公开(公告)号：US09023766B2

    公开(公告)日：2015-05-05

    申请号：US12009290

    申请日：2008-03-07

    申请人： Yingjian Wang

    发明人： Yingjian Wang

    IPC分类号： C40B30/04 ,  G01N33/53 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68

    CPC分类号： C12Q1/6837 ,  C40B30/04 ,  G01N33/5005 ,  G01N33/543 ,  G01N33/6842 ,  G01N33/6845 ,  C12Q2565/515

    摘要： Arrays and methods for detecting one or more biological molecules, where the methods generally comprise the steps of: providing a first support structure fixed with one or more reagents; providing a second support structure fixed with a plurality of ligands; contacting the reagents fixed to the first support structure with the ligands fixed to the second support structure whereby a plurality of the reagents bind to one or more of the ligands; and separating the first support structure from the second support structure so that the plurality of the bound reagents remain bound to a plurality of ligands on the second support after separation. In one preferred method, proteins are immobilized on a support with adequate strength so that the proteins can be dissociated from the support under certain conditions, such as after binding with other proteins immobilized on another support. For example, antibody arrays produced according to the present invention may be used to detect protein expressions in a protein lysate and may be used in immunostaining to reveal the presence and location of protein in cells.

    摘要翻译： 用于检测一种或多种生物分子的阵列和方法，其中所述方法通常包括以下步骤：提供用一种或多种试剂固定的第一支持结构; 提供固定有多个配体的第二支持结构; 使固定到第一支撑结构上的试剂与固定在第二支撑结构上的配体接触，由此多个试剂与一种或多种配体结合; 以及将第一支撑结构与第二支撑结构分离，使得多个结合的试剂在分离后保持与第二支持体上的多个配体结合。 在一个优选的方法中，将蛋白质固定在具有足够强度的载体上，使得蛋白质可以在某些条件下，例如在与另一种载体上固定的其它蛋白质结合后，从支持物解离。 例如，根据本发明产生的抗体阵列可用于检测蛋白质裂解物中的蛋白质表达，并且可用于免疫染色以揭示蛋白质在细胞中的存在和位置。