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    ENCAPSULATION OF EUKARYOTIC CELLS FOR CELLULAR SCREENING OF EXPRESSED SEQUENCES
    3.
    发明申请
    ENCAPSULATION OF EUKARYOTIC CELLS FOR CELLULAR SCREENING OF EXPRESSED SEQUENCES 有权

    公开(公告)号：US20210054530A1

    公开(公告)日：2021-02-25

    申请号：US16612132

    申请日：2018-05-11

    申请人： The Florey Institute of Neuroscience and Mental Health

    发明人： Daniel James Scott ,  Ross Bathgate

    IPC分类号： C40B30/04 ,  C40B20/00 ,  C40B40/02 ,  G01N33/68

    摘要： Provided herein are methods for selecting polypeptides or proteins having one or more desired properties from a library of sequences expressed in eukaryotic cells, comprising: encapsulating said cells by photopolymerization; solubilizing said encapsulated cells to produce semipermeable microcapsules; optionally contacting said cells and/or said microcapsules with one or more agents to facilitate detection of activity or function of polypeptides or proteins of interest; and selecting polypeptides or proteins of interest having one or more desired properties. Also provided are methods for encapsulating eukaryotic cells for use in the selection of polypeptides and proteins as described above.

    Methods and kits for theranostic applications
    4.
    发明授权
    Methods and kits for theranostic applications 有权

    公开(公告)号：US10704084B2

    公开(公告)日：2020-07-07

    申请号：US15529807

    申请日：2015-12-02

    申请人： TriBiotica LLC

    发明人： Ian Dunn ,  Matthew Lawler

    IPC分类号： C12Q1/68 ,  C12Q1/6816 ,  C12N15/10 ,  C40B20/00 ,  C40B40/00

    摘要： The present disclosure is directed to methods and kits for identifying, enriching, and evaluating templated assembly reactants. Some embodiments disclose methods for identifying templated assembly targets by synthesizing templated assembly reactants, hybridizing the templated assembly reactants to target nucleic acids, performing a templated assembly reaction, and identifying the target nucleic acids that hybridized to the templated assembly reactants. Libraries of templated assembly reactants, a kit for identifying templated assembly targets, and a pair of templated assembly targets enriched from a library of chemically-ligated oligonucleotides spatially elicited (CLOSE) products are also disclosed.

    Methods and materials using signaling probes
    9.
    发明授权
    Methods and materials using signaling probes 有权
    使用信号探针的方法和材料

    公开(公告)号：US08916503B2

    公开(公告)日：2014-12-23

    申请号：US10589052

    申请日：2005-02-17

    申请人： Kambiz Shekdar ,  Dennis J. Sawchuk ,  Jason M. Montez

    发明人： Kambiz Shekdar ,  Dennis J. Sawchuk ,  Jason M. Montez

    IPC分类号： C40B20/00 ,  C12Q1/68 ,  C12N15/00

    CPC分类号： C12Q1/6841 ,  C12Q2525/207 ,  C12Q2525/301 ,  C12Q2563/137 ,  C12Q2563/155 ,  C12Q2565/1015 ,  C12Q2565/102

    摘要： Methods of isolating cells or generating cell lines comprising the step of exposing the cells to signaling probes that produce a signal upon hybridization to a target sequence, as well as methods of quantifying the level of expression of an RNA of interest, methods for identifying genetic recombinational events in living cells and methods of generating a transgenic animal using the isolated cells. Methods for isolating a plurality of cells encoding a plurality of different RNAs associated with a same nucleic acid tag sequence, comprising the step of exposing the cells to a same signaling probe that produces a detectable signal upon hybridization to the same nucleic acid tag sequence, are also provided. Signaling probes and protease probes that form stem-loop structures, three-arm junction structures, and dumbbell structures may be used in the above methods.

    摘要翻译： 分离细胞或产生细胞系的方法包括将细胞暴露于与靶序列杂交时产生信号的信号传导探针的步骤，以及定量目的RNA表达水平的方法，用于鉴定遗传重组的方法 活细胞中的事件和使用分离的细胞产生转基因动物的方法。 用于分离编码与相同核酸标签序列相关联的多个不同RNA的多个细胞的方法包括将细胞暴露于与同一核酸标签序列杂交时产生可检测信号的相同信号探针的步骤是 也提供。 在上述方法中可以使用形成茎环结构，三臂连接结构和哑铃结构的信号探针和蛋白酶探针。