公开(公告)号：US20240336964A1

公开(公告)日：2024-10-10

申请号：US18739789

申请日：2024-06-11

申请人： Sarmal, Inc.

发明人： Inanc ORTAC

IPC分类号： C12Q1/686 ,  C12Q1/6818 ,  G01N21/66

CPC分类号： C12Q1/686 ,  C12Q1/6818 ,  G01N21/66 ,  C12Q2565/301

摘要： Provided herein are methods and systems for detecting the presence of absence of a target-nucleic acid sequence, including SARS-COV2.

公开(公告)号：US12059677B2

公开(公告)日：2024-08-13

申请号：US16971967

申请日：2019-04-12

申请人： ZHANGJIAGANG ONE-CHIP BIOTECHNOLOGY CO., LTD.

发明人： Yabao Liu ,  Yaofei Yue ,  Zhifeng Zhang

IPC分类号： C12Q1/6806 ,  B01L3/00 ,  C12N15/10 ,  C12Q1/6874

CPC分类号： B01L3/5027 ,  C12N15/1096 ,  C12Q1/6806 ,  C12Q1/6874 ,  B01L2300/0829 ,  C12Q1/6874 ,  C12Q2531/113 ,  C12Q2563/159 ,  C12Q2565/301 ,  C12Q2565/519 ,  C12Q1/6806 ,  C12Q2531/113 ,  C12Q2563/159 ,  C12Q2565/301 ,  C12Q2565/519

摘要： Provided is a method of gene sequencing on a microwell array chip, including: Step 1: adding a PCR amplification system containing DNA fragments to be sequenced into the microwell array chip, allowing microwells to each individually form reaction spaces and allowing one DNA fragment to be contained in one microwell; Steps 2 to 3: subjecting the microwell array chip to PCR amplification, and denaturing amplified double-stranded DNAs in individual microwells; and Steps 4 and 5: sequencing the DNA fragments with sequencing primer S2 molecules and dNTPs in each microwell with a sensor at the bottom of the microwell.

公开(公告)号：US11845981B2

公开(公告)日：2023-12-19

申请号：US17586596

申请日：2022-01-27

申请人： Shaofeng Ding ,  Qiang Liu

发明人： Shaofeng Ding ,  Qiang Liu

IPC分类号： C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6858

CPC分类号： C12Q1/6853 ,  C12Q1/686 ,  C12Q2525/186 ,  C12Q2565/301

摘要： A minimal-copy-ratio of templates is a problem in detecting early stage cancer where minimal copies of somatic cancer-specific mutations are targeted in the presence of large copies of wildtype genome DNA, commonly a 1/10,000 or even less minimal-copy-ratios between the mutant target and wildtype control templates. To overcome this problem, delayed pyrophosphorolysis activated polymerization (delayed-PAP) was developed which can delay product accumulation of the wildtype control to a much later time or cycle, such as by 15 cycles or by 30,000 folds. In the multiplex format, delayed-PAP is particularly useful to amplify not only the wildtype control but also mutant target templates accurately and consistently in the minimal-copy-ratio situation.

公开(公告)号：US20180334708A1

公开(公告)日：2018-11-22

申请号：US15949456

申请日：2018-04-10

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Jonathan ROTHBERG ,  Wolfgang HINZ ,  Kim JOHNSON ,  James BUSTILLO

IPC分类号： C12Q1/6869 ,  G01N27/414 ,  H01L27/088 ,  H01L23/00 ,  C12Q1/6874 ,  H01L21/306 ,  H01L29/78

CPC分类号： C12Q1/6869 ,  C12Q1/6874 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4148 ,  H01L21/306 ,  H01L24/18 ,  H01L24/20 ,  H01L24/82 ,  H01L27/088 ,  H01L29/78 ,  H01L2224/04105 ,  H01L2224/16 ,  H01L2224/76155 ,  H01L2224/82102 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01011 ,  H01L2924/01012 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01019 ,  H01L2924/0102 ,  H01L2924/01023 ,  H01L2924/01024 ,  H01L2924/01027 ,  H01L2924/01029 ,  H01L2924/0103 ,  H01L2924/01033 ,  H01L2924/01046 ,  H01L2924/01047 ,  H01L2924/01052 ,  H01L2924/01056 ,  H01L2924/01059 ,  H01L2924/01073 ,  H01L2924/01074 ,  H01L2924/01075 ,  H01L2924/01082 ,  H01L2924/1306 ,  H01L2924/13091 ,  H01L2924/14 ,  H01L2924/1433 ,  H01L2924/19041 ,  H01L2924/19042 ,  H01L2924/19043 ,  H01L2924/30105 ,  H01L2924/3011 ,  H01L2924/3025 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101 ,  H01L2924/00

摘要： Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US09909177B2

公开(公告)日：2018-03-06

申请号：US14850705

申请日：2015-09-10

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Jian Wu ,  Dae H. Kim

IPC分类号： C12Q1/6869 ,  C12Q1/68 ,  C12Q1/66 ,  G01N33/53 ,  G01N33/543

CPC分类号： C12Q1/6874 ,  C12Q1/66 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2334/20 ,  G01N33/5308 ,  G01N33/54353 ,  C12Q2525/113 ,  C12Q2565/301 ,  C12Q2525/186

摘要： This invention provides methods for pyrosequencing and compositions comprising 3′-O-modified deoxynucleoside triphosphates.

公开(公告)号：US20170321272A1

公开(公告)日：2017-11-09

申请号：US15594994

申请日：2017-05-15

申请人： DNAE Group Holdings Limited

发明人： Pantelis GEORGIOU ,  Samuel REED ,  Yan LIU ,  Christofer TOUMAZOU ,  Chung-Pei OU

IPC分类号： C12Q1/68 ,  G01N27/414 ,  G01N27/44

CPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6858 ,  G01N27/4145 ,  G01N27/44 ,  C12Q2533/101 ,  C12Q2565/301 ,  C12Q2565/607 ,  C12Q2565/501

摘要： An apparatus with a transducer having a first output signal and arranged to receive an electrical input. The transducer switches the first output signal between an ON and OFF state. The apparatus has a chemical sensing surface coupled to the transducer arranged to receive a chemical input. A signal generator oscillates one or more of said inputs to vary the switching point of the transducer. The oscillating input may be the chemical input and/or the electrical input. The output signal may be a pulse whose period ON or OFF is determined by the oscillating input modulated by the chemical input.

公开(公告)号：US09797005B2

公开(公告)日：2017-10-24

申请号：US12094952

申请日：2006-11-22

申请人： Mihaela Campan ,  Peter W. Laird ,  Allen S. Yang ,  Hui-Lee Wong

发明人： Mihaela Campan ,  Peter W. Laird ,  Allen S. Yang ,  Hui-Lee Wong

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6858 ,  C12Q2565/301 ,  C12Q2545/114 ,  C12Q2523/125

摘要： Particular aspects provide novel, high-throughput methods to quantify DNA methylation (e.g., at a single-base resolution) in an allele-specific manner. The methods comprise use of an allele-specific sequence polymorphism (e.g., allele-specific single nucleotide polymorphism; SNP) in sufficient proximity to a CpG methylation site to provide for distinguishing the methylation levels between two alleles. In particular aspects, after bisulfite modification, the genomic DNA region is PCR-amplified, and the product subjected to allele-specific pyrosequencing, and the percentage of methylation determined based on the percentage of cytosine to thymidine conversion. In further embodiments, MethyLight™ is used after bisulfite treatment. The inventive methodology has, for example, substantial utility for affording quantitative analyses in the regulation of analyses of X-inactivation, the allele-specific expression of genes (e.g., in the immune system) and junk DNA, etc., and in classifying an individual as to whether they have loss of imprinting (LOI).

公开(公告)号：US09777323B2

公开(公告)日：2017-10-03

申请号：US14404964

申请日：2013-06-13

申请人： Siemens Aktiengesellschaft

发明人： Walter Gumbrecht ,  Oliver Hayden

IPC分类号： C12Q1/68 ,  G01N27/414

CPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  G01N27/4145 ,  C12Q2565/301 ,  C12Q2565/607

摘要： An assembly and a method are disclosed for analyzing nucleic acid sequences by way of so-called sequencing-by-synthesis. According to an embodiment of the invention, a chemical substance group that is released when a nucleotide bonds to a nucleic acid sequence to be sequenced is detected. The reagents are applied by way of a spraying device to a sensor that detects the released substance group. This has the advantage that no lateral flow occurs. The rate of false-negative and false-positive results is significantly reduced. Furthermore, a small amount of the reagent is sufficient to completely wet the sensor. Filling of the supply and discharge lines as for a flow cell is not necessary.

公开(公告)号：US09702004B2

公开(公告)日：2017-07-11

申请号：US15366417

申请日：2016-12-01

申请人： Keygene N.V.

发明人： Michael Josephus Theresia Van Eijk ,  Anker Preben Sørensen ,  Marco Gerardus Maria Van Schriek

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04 ,  C40B50/16 ,  C40B50/18

CPC分类号： C12Q1/6874 ,  C12Q1/6827 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2565/301 ,  C12Q2535/138 ,  C12Q2535/122 ,  C12Q2527/107

摘要： The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.

公开(公告)号：US09695466B2

公开(公告)日：2017-07-04

申请号：US14274168

申请日：2014-05-09

申请人： Harry Cuppens

发明人： Harry Cuppens

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q2525/151 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2525/185 ,  C12Q2535/113 ,  C12Q2565/301

摘要： The invention relates to methods to reduce the length of a homonucleotide repeat sequence a target DNA sequence. This is done by using an oligonucleotide primer which extends in the unaltered repeat of identical nucleotides of the target polynucleotide, the oligonucleotide primer comprises at the at the 5′ side a sequence which hybridizes with the sequence preceding the targeted sequence repeat, but the oligonucleotide primer is not 100% complementary with the sequence repeat in the target sequence.