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    NUCLEIC ACID AMPLIFICATION
    1.
    发明申请
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20190338258A1

    公开(公告)日：2019-11-07

    申请号：US16442341

    申请日：2019-06-14

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Bin LI ,  Kai Qin LAO

    IPC分类号： C12N9/12 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6855

    摘要： The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

    NUCLEIC ACID AMPLIFICATION
    3.
    发明申请
    NUCLEIC ACID AMPLIFICATION 有权

    公开(公告)号：US20140147852A1

    公开(公告)日：2014-05-29

    申请号：US14085727

    申请日：2013-11-20

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Chieh-Yuan LI ,  David RUFF ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Shiaw-Min CHEN ,  Jonathan ROTHBERG

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

    摘要： In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    METHODS AND APPARATUS FOR DETECTING MOLECULAR INTERACTIONS USING FET ARRAYS
    4.
    发明申请
    METHODS AND APPARATUS FOR DETECTING MOLECULAR INTERACTIONS USING FET ARRAYS 审中-公开
    使用FET阵列检测分子相互作用的方法和装置

    公开(公告)号：US20130280813A1

    公开(公告)日：2013-10-24

    申请号：US13922484

    申请日：2013-06-20

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Jonathan ROTHBERG ,  Wolfgang HINZ

    IPC分类号： G01N27/26

    CPC分类号： C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/4145 ,  G01N27/4148 ,  G01N33/54373 ,  G01N33/6818 ,  H01L29/78 ,  H01L29/788 ,  H01L2924/01006

    摘要： Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

    摘要翻译： 提供了与用于分析物检测和测量的大规模FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。

    APPARATUS AND METHODS FOR PERFORMING ELECTROCHEMICAL REACTIONS
    8.
    发明申请
    APPARATUS AND METHODS FOR PERFORMING ELECTROCHEMICAL REACTIONS 审中-公开
    用于执行电化学反应的装置和方法

    公开(公告)号：US20140202883A1

    公开(公告)日：2014-07-24

    申请号：US14162612

    申请日：2014-01-23

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： John NOBILE ,  George T. ROTH ,  Todd REARICK ,  Jonathan M. SCHULTZ ,  Jonathan ROTHBERG ,  David MARRAN

    IPC分类号： G01N27/30

    CPC分类号： G01N27/301 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/005 ,  B01J2219/00653 ,  B01J2219/00722 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  G01N27/4145

    摘要： The invention is directed to apparatus and methods for delivering multiple reagents to, and monitoring, a plurality of analytical reactions carried out on a large-scale array of electronic sensors under minimal noise conditions. In one aspect, the invention provides method of improving signal-to-noise ratios of output signals from the electronic sensors sensing analytes or reaction byproducts by subtracting an average of output signals measured from neighboring sensors where analyte or reaction byproducts are absent. In other aspects, the invention provides an array of electronic sensors integrated with a microwell array for confining analytes and/or particles for analytical reactions and a method for identifying microwells containing analytes and/or particles by passing a sensor-active reagent over the array and correlating sensor response times to the presence or absence of analytes or particles. Such detection of analyte- or particle-containing microwells may be used as a step in additional noise reduction methods.

    摘要翻译： 本发明涉及用于在最小噪声条件下向多个电子传感器阵列上进行的多个分析反应的多个试剂的输送和监测的装置和方法。 一方面，本发明提供了通过减去从相邻传感器测得的输出信号的平均值来提高来自电子传感器感测分析物或反应副产物的输出信号的信噪比的方法，其中分析物或反应副产物不存在。 在其它方面，本发明提供了与微孔阵列集成的电子传感器阵列，用于限制用于分析反应的分析物和/或颗粒，以及通过使传感器活性试剂通过阵列而识别含有分析物和/或颗粒的微孔的方法，以及 将传感器响应时间与分析物或颗粒的存在或不存在相关联。 含有分析物或含微粒微孔的这种检测可以用作额外的降噪方法中的步骤。

    NUCLEIC ACID AMPLIFICATION
    9.
    发明申请
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20140148345A1

    公开(公告)日：2014-05-29

    申请号：US14085718

    申请日：2013-11-20

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Chieh-Yuan LI ,  David RUFF ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Shiaw-Min CHEN ,  Jonathan ROTHBERG

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6846 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537 ,  C12Q2563/159 ,  C12Q2565/513 ,  C12Q2565/519 ,  C12Q2565/607

    摘要： In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    METHODS AND APPARATUS FOR DETECTING MOLECULAR INTERACTIONS USING FET ARRAYS
    10.
    发明申请
    METHODS AND APPARATUS FOR DETECTING MOLECULAR INTERACTIONS USING FET ARRAYS 有权
    使用FET阵列检测分子相互作用的方法和装置

    公开(公告)号：US20140080719A1

    公开(公告)日：2014-03-20

    申请号：US14033934

    申请日：2013-09-23

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Jonathan ROTHBERG ,  Wolfgang Hinz

    IPC分类号： G01N33/68

    CPC分类号： C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/4145 ,  G01N27/4148 ,  G01N33/54373 ,  G01N33/6818 ,  H01L29/78 ,  H01L29/788 ,  H01L2924/01006

    摘要： Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

    摘要翻译： 提供了与用于分析物检测和测量的大规模FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。