知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

30 条记录 (耗时 0.047 秒)

    Emulsion chemistry for encapsulated droplets
    3.
    发明授权
    Emulsion chemistry for encapsulated droplets 有权

    公开(公告)号：US10378048B2

    公开(公告)日：2019-08-13

    申请号：US15452582

    申请日：2017-03-07

    申请人： Bio-Rad Laboratories, Inc.

    发明人： Amy L. Hiddessen ,  Benjamin J. Hindson

    IPC分类号： C12Q1/68 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/686

    摘要： System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.

    SILICONE SURFACTANTS FOR EMULSION ASSAYS
    4.
    发明申请
    SILICONE SURFACTANTS FOR EMULSION ASSAYS 有权
    用于乳化剂测定的硅氧烷表面活性剂

    公开(公告)号：US20140272995A1

    公开(公告)日：2014-09-18

    申请号：US14216006

    申请日：2014-03-17

    申请人： Bio-Rad Laboratories, Inc.

    发明人： Erin R. Chia ,  Amy L. Hiddessen ,  Benjamin J. Hindson ,  Adam Lowe ,  Chunxiao Han

    IPC分类号： C12Q1/68 ,  C07F7/18

    CPC分类号： C07F7/1804 ,  C08G77/46 ,  C08G77/50 ,  C08L83/12 ,  C08L83/14 ,  G01N33/5436

    摘要： System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.

    摘要翻译： 用于制备和使用包括硅油和硅氧烷表面活性剂的乳液的方法和组合物的系统。 乳液可以包括设置在包括硅油和硅氧烷表面活性剂的连续相中的水性液滴。 水滴可以含有任选地部分占据的分析物和/或发光（例如光致发光）报告物。 分析物的测定可以用液滴进行。 在一些情况下，可以从液滴中检测信号，并且可以基于该信号来确定分析物的特征，例如分析物水平或活性。

    Stabilized droplets for calibration and testing
    5.
    发明授权
    Stabilized droplets for calibration and testing 有权

    公开(公告)号：US10240187B2

    公开(公告)日：2019-03-26

    申请号：US15389116

    申请日：2016-12-22

    申请人： Bio-Rad Laboratories, Inc.

    发明人： Amy L. Hiddessen ,  Erin R. Chia

    IPC分类号： C12Q1/68 ,  B01F13/00 ,  B01F5/06 ,  G01N1/38 ,  G01N21/64 ,  C12Q1/6816 ,  B01F5/00 ,  B01F9/00 ,  B01F11/00 ,  C12Q1/6806 ,  B01L3/00 ,  G01N1/28

    摘要： Method of detecting a signal from droplets for instrument calibration. In the method, a calibration standard for a droplet detection instrument may be received. The calibration standard may have been shipped at least one kilometer when received. The calibration standard may include a mixture of first droplets and second droplets. Each of the first and second droplets may be encapsulated by an immiscible carrier liquid and may have a stabilizing droplet skin that is formed by heating and that includes a skin-forming protein. Each the first droplets may contain a fluorescent dye, and each of the second droplets may contain a fluorescent dye. A fluorescence signal may be detected from a plurality of the first and second droplets with a droplet detection instrument. The fluorescence signal may be stronger for the first droplets than the second droplets.

    Systems and methods for stabilizing droplets
    10.
    发明授权
    Systems and methods for stabilizing droplets 有权
    用于稳定液滴的系统和方法

    公开(公告)号：US09328376B2

    公开(公告)日：2016-05-03

    申请号：US14018205

    申请日：2013-09-04

    申请人： BIO-RAD LABORATORIES, INC.

    发明人： Amy L. Hiddessen ,  Erin R. Chia

    IPC分类号： C12Q1/68 ,  B01L3/00 ,  B01L7/00

    CPC分类号： C12Q1/6806 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0487

    摘要： This disclosure provides systems, devices, and methods for sample preparation and/or analysis comprising a droplet generator having a first channel in fluid communication with a carrier fluid reservoir and a second channel in fluid communication with a sample reservoir. The first channel and second channel may meet at an intersection that receives a sample from the sample reservoir and a carrier fluid from the carrier fluid reservoir and generates one or more droplets that flow along a droplet channel to a droplet reservoir. An energy application member in thermal communication with the intersection, at least a portion of the droplet channel, the sample reservoir and/or the carrier fluid reservoir may provide energy to an individual droplet of the one or more droplets, such as at the intersection and/or as the droplet moves along the droplet channel to the droplet reservoir.

    摘要翻译： 本公开提供了用于样品制备和/或分析的系统，装置和方法，其包括具有与载体流体储存器流体连通的第一通道和与样品储存器流体连通的第二通道的液滴发生器。 第一通道和第二通道可以在从样品储存器接收样品和来自载体流体储存器的载体流体的交叉点处相遇，并且产生沿着液滴通道流动到液滴储存器的一个或多个液滴。 与交叉点热连通的能量施加构件，液滴通道，样品储存器和/或载体流体储存器的至少一部分可以向一个或多个液滴的单个液滴（例如在交叉点处）提供能量，并且 /或当液滴沿着液滴通道移动到液滴储存器时。